The Effect of Inhibition of Dopamine D2 Receptors on Some of the Peripheral Blood Mononuclear Cells of the Rat under Food restriction
Background & Objective: In previous studies, the effects of food restriction on the changes in immune responses and brain dopamine content have been determined. On the other hand, it has been shown that immune cells, in addition to dopamine production, also have dopamine receptors. The purpose o...
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| Format: | Article |
| Language: | English |
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Fasa University of Medical Sciences
2018-12-01
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| Series: | Journal of Advanced Biomedical Sciences |
| Subjects: | |
| Online Access: | http://jabs.fums.ac.ir/article-1-1626-en.pdf |
| _version_ | 1797679646304632832 |
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| author | khatereh Noormohammadi Farrin Babaei Balderlou Seyyed Meysam Abtahi Foroushani |
| author_facet | khatereh Noormohammadi Farrin Babaei Balderlou Seyyed Meysam Abtahi Foroushani |
| author_sort | khatereh Noormohammadi |
| collection | DOAJ |
| description | Background & Objective: In previous studies, the effects of food restriction on the changes in immune responses and brain dopamine content have been determined. On the other hand, it has been shown that immune cells, in addition to dopamine production, also have dopamine receptors. The purpose of this study was to evaluate the effect of inhibition of D2 dopamine receptors on several functions of monocytes of peripheral blood in rat under food restriction
Materials & methods: In this experimental study, 36 male Wistar rats (weighing 200-250 gr) were allocated into six groups (n=6), including control groups, food restriction (25%), food restriction (50%), food restriction (75%), food restriction 75% and Sulpiride and rats treated with Sulpiride. Sulpiride was injected Intracerebroventricular at a concentration of 50 μg / rat on day 21 after the study initiation. At the end, the Rats were bled and peripheral blood mononuclear cells were isolated by ficoll gradient method.
Results: Food restriction caused a significant decrease in the activity of monocyte cells of gradient of peripheral blood mononuclear cells like neutral red uptake test and respiratory burst (NBT reduction test) simultaneously with decreasing lymphocytes proliferation after stimulation with phytohemagglutinin. Administration of Sulpiride with a 75% Food restriction resulted in the improvement of these functions of monocyte cells of gradient of peripheral blood mononuclear cells as well as lymphocyte proliferation.
Conclusion: Intracerebroventricular administration of dopamine D2 receptor antagonists (sulpiride) effectively inhibited the effects of a severe dietary restriction on the suppression of immunity system. |
| first_indexed | 2024-03-11T23:17:50Z |
| format | Article |
| id | doaj.art-b10c36f2ccb241fd9547e82439689e9a |
| institution | Directory Open Access Journal |
| issn | 2228-5105 2783-1523 |
| language | English |
| last_indexed | 2024-03-11T23:17:50Z |
| publishDate | 2018-12-01 |
| publisher | Fasa University of Medical Sciences |
| record_format | Article |
| series | Journal of Advanced Biomedical Sciences |
| spelling | doaj.art-b10c36f2ccb241fd9547e82439689e9a2023-09-20T21:25:43ZengFasa University of Medical SciencesJournal of Advanced Biomedical Sciences2228-51052783-15232018-12-018411461156The Effect of Inhibition of Dopamine D2 Receptors on Some of the Peripheral Blood Mononuclear Cells of the Rat under Food restrictionkhatereh Noormohammadi0Farrin Babaei Balderlou1Seyyed Meysam Abtahi Foroushani2 Department of Biology, Faculty of Sciences, Urmia University, Urmia, Iran Department of Biology, Faculty of Sciences, Urmia University, Urmia, Iran Department of Microbiology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran Background & Objective: In previous studies, the effects of food restriction on the changes in immune responses and brain dopamine content have been determined. On the other hand, it has been shown that immune cells, in addition to dopamine production, also have dopamine receptors. The purpose of this study was to evaluate the effect of inhibition of D2 dopamine receptors on several functions of monocytes of peripheral blood in rat under food restriction Materials & methods: In this experimental study, 36 male Wistar rats (weighing 200-250 gr) were allocated into six groups (n=6), including control groups, food restriction (25%), food restriction (50%), food restriction (75%), food restriction 75% and Sulpiride and rats treated with Sulpiride. Sulpiride was injected Intracerebroventricular at a concentration of 50 μg / rat on day 21 after the study initiation. At the end, the Rats were bled and peripheral blood mononuclear cells were isolated by ficoll gradient method. Results: Food restriction caused a significant decrease in the activity of monocyte cells of gradient of peripheral blood mononuclear cells like neutral red uptake test and respiratory burst (NBT reduction test) simultaneously with decreasing lymphocytes proliferation after stimulation with phytohemagglutinin. Administration of Sulpiride with a 75% Food restriction resulted in the improvement of these functions of monocyte cells of gradient of peripheral blood mononuclear cells as well as lymphocyte proliferation. Conclusion: Intracerebroventricular administration of dopamine D2 receptor antagonists (sulpiride) effectively inhibited the effects of a severe dietary restriction on the suppression of immunity system.http://jabs.fums.ac.ir/article-1-1626-en.pdfdopamineperipheral blood mononuclear cellsfood restrictionsulpiriderat |
| spellingShingle | khatereh Noormohammadi Farrin Babaei Balderlou Seyyed Meysam Abtahi Foroushani The Effect of Inhibition of Dopamine D2 Receptors on Some of the Peripheral Blood Mononuclear Cells of the Rat under Food restriction Journal of Advanced Biomedical Sciences dopamine peripheral blood mononuclear cells food restriction sulpiride rat |
| title | The Effect of Inhibition of Dopamine D2 Receptors on Some of the Peripheral Blood Mononuclear Cells of the Rat under Food restriction |
| title_full | The Effect of Inhibition of Dopamine D2 Receptors on Some of the Peripheral Blood Mononuclear Cells of the Rat under Food restriction |
| title_fullStr | The Effect of Inhibition of Dopamine D2 Receptors on Some of the Peripheral Blood Mononuclear Cells of the Rat under Food restriction |
| title_full_unstemmed | The Effect of Inhibition of Dopamine D2 Receptors on Some of the Peripheral Blood Mononuclear Cells of the Rat under Food restriction |
| title_short | The Effect of Inhibition of Dopamine D2 Receptors on Some of the Peripheral Blood Mononuclear Cells of the Rat under Food restriction |
| title_sort | effect of inhibition of dopamine d2 receptors on some of the peripheral blood mononuclear cells of the rat under food restriction |
| topic | dopamine peripheral blood mononuclear cells food restriction sulpiride rat |
| url | http://jabs.fums.ac.ir/article-1-1626-en.pdf |
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