Quantitative Acetylome Analysis of Differentially Modified Proteins in Virulence-Differentiated <i>Fusarium oxysporum</i> f. sp. <i>cucumerinum</i> Isolates during Cucumber Colonization
<i>Fusarium oxysporum</i> f. sp. <i>cucumerinum</i> (<i>Foc</i>) is a prominent pathogen that adversely affects cucumber (<i>Cucumis sativus</i>) production. In the pathogen’s parasitic lifestyle, the pathogenesis and virulence evolution may be regulat...
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MDPI AG
2023-09-01
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| Series: | Journal of Fungi |
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| Online Access: | https://www.mdpi.com/2309-608X/9/9/920 |
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| author | Ying Zhou Xiaohong Lu Jianjun Hao Shidong Li |
| author_facet | Ying Zhou Xiaohong Lu Jianjun Hao Shidong Li |
| author_sort | Ying Zhou |
| collection | DOAJ |
| description | <i>Fusarium oxysporum</i> f. sp. <i>cucumerinum</i> (<i>Foc</i>) is a prominent pathogen that adversely affects cucumber (<i>Cucumis sativus</i>) production. In the pathogen’s parasitic lifestyle, the pathogenesis and virulence evolution may be regulated by lysine acetylation, as demonstrated in many living organisms. However, its specific function in <i>Foc</i> remains poorly understood. In this study, the acetylome profiles of a mild virulence strain (foc-3b) and its derived virulence-enhanced strain (Ra-4) were analyzed before and post-inoculation on cucumber plants. In total, 10,664 acetylation sites were identified corresponding to 3874 proteins, and 45 conserved acetylation motifs were detected. Through comparison of the acetylomes, numerous differentially lysine-acetylated proteins were enriched in energy metabolism and protein processing processes, indicating the critical role of lysine acetylation during the transition from the saprotrophic lifestyle to the parasitic lifestyle. Comparative acetylome analyses on the two virulence-differentiated strains revealed that several differentially lysine-acetylated proteins were involved in pathways of defense response and energy metabolism. Ra-4 showed enhanced energy metabolism compared to foc-3b. This indicates that robust metabolic activity is required to achieve high virulence and facilitating adaptive evolution. Additionally, faster host responses are supported by an ample energy supply enhancing virulence. Thus, lysine acetylation plays a crucial role in the pathogenesis and virulence evolution of <i>Foc</i>. |
| first_indexed | 2024-03-10T22:35:25Z |
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| language | English |
| last_indexed | 2024-03-10T22:35:25Z |
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| spelling | doaj.art-b113ec43747d4ddbb5248575a29b4cd62023-11-19T11:29:08ZengMDPI AGJournal of Fungi2309-608X2023-09-019992010.3390/jof9090920Quantitative Acetylome Analysis of Differentially Modified Proteins in Virulence-Differentiated <i>Fusarium oxysporum</i> f. sp. <i>cucumerinum</i> Isolates during Cucumber ColonizationYing Zhou0Xiaohong Lu1Jianjun Hao2Shidong Li3State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, ChinaState Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, ChinaSchool of Food and Agriculture, University of Maine, Orono, ME 04469, USAState Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China<i>Fusarium oxysporum</i> f. sp. <i>cucumerinum</i> (<i>Foc</i>) is a prominent pathogen that adversely affects cucumber (<i>Cucumis sativus</i>) production. In the pathogen’s parasitic lifestyle, the pathogenesis and virulence evolution may be regulated by lysine acetylation, as demonstrated in many living organisms. However, its specific function in <i>Foc</i> remains poorly understood. In this study, the acetylome profiles of a mild virulence strain (foc-3b) and its derived virulence-enhanced strain (Ra-4) were analyzed before and post-inoculation on cucumber plants. In total, 10,664 acetylation sites were identified corresponding to 3874 proteins, and 45 conserved acetylation motifs were detected. Through comparison of the acetylomes, numerous differentially lysine-acetylated proteins were enriched in energy metabolism and protein processing processes, indicating the critical role of lysine acetylation during the transition from the saprotrophic lifestyle to the parasitic lifestyle. Comparative acetylome analyses on the two virulence-differentiated strains revealed that several differentially lysine-acetylated proteins were involved in pathways of defense response and energy metabolism. Ra-4 showed enhanced energy metabolism compared to foc-3b. This indicates that robust metabolic activity is required to achieve high virulence and facilitating adaptive evolution. Additionally, faster host responses are supported by an ample energy supply enhancing virulence. Thus, lysine acetylation plays a crucial role in the pathogenesis and virulence evolution of <i>Foc</i>.https://www.mdpi.com/2309-608X/9/9/920<i>Cucumis sativus</i>lysine acetylationproteomepathogenesisvirulence evolution |
| spellingShingle | Ying Zhou Xiaohong Lu Jianjun Hao Shidong Li Quantitative Acetylome Analysis of Differentially Modified Proteins in Virulence-Differentiated <i>Fusarium oxysporum</i> f. sp. <i>cucumerinum</i> Isolates during Cucumber Colonization Journal of Fungi <i>Cucumis sativus</i> lysine acetylation proteome pathogenesis virulence evolution |
| title | Quantitative Acetylome Analysis of Differentially Modified Proteins in Virulence-Differentiated <i>Fusarium oxysporum</i> f. sp. <i>cucumerinum</i> Isolates during Cucumber Colonization |
| title_full | Quantitative Acetylome Analysis of Differentially Modified Proteins in Virulence-Differentiated <i>Fusarium oxysporum</i> f. sp. <i>cucumerinum</i> Isolates during Cucumber Colonization |
| title_fullStr | Quantitative Acetylome Analysis of Differentially Modified Proteins in Virulence-Differentiated <i>Fusarium oxysporum</i> f. sp. <i>cucumerinum</i> Isolates during Cucumber Colonization |
| title_full_unstemmed | Quantitative Acetylome Analysis of Differentially Modified Proteins in Virulence-Differentiated <i>Fusarium oxysporum</i> f. sp. <i>cucumerinum</i> Isolates during Cucumber Colonization |
| title_short | Quantitative Acetylome Analysis of Differentially Modified Proteins in Virulence-Differentiated <i>Fusarium oxysporum</i> f. sp. <i>cucumerinum</i> Isolates during Cucumber Colonization |
| title_sort | quantitative acetylome analysis of differentially modified proteins in virulence differentiated i fusarium oxysporum i f sp i cucumerinum i isolates during cucumber colonization |
| topic | <i>Cucumis sativus</i> lysine acetylation proteome pathogenesis virulence evolution |
| url | https://www.mdpi.com/2309-608X/9/9/920 |
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