Fluorescence Quenching of Graphene Quantum Dots by Chloride Ions: A Potential Optical Biosensor for Cystic Fibrosis
Cystic fibrosis is a genetic disorder caused by a mutation in the cystic fibrosis transmembrane conductance regulator (CFTR) gene that encodes an ABC transporter-class ion channel protein, resulting in sticky and thick mucus secretion which clogs the airways and leads to respiratory failure and othe...
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| Format: | Article |
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Frontiers Media S.A.
2022-04-01
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| Series: | Frontiers in Materials |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmats.2022.857432/full |
| _version_ | 1830132268079775744 |
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| author | Zahid Ifrah Abbas Shah Rukh Sharif Muhammad Nauman Shahid Maryam Ullah Rahat |
| author_facet | Zahid Ifrah Abbas Shah Rukh Sharif Muhammad Nauman Shahid Maryam Ullah Rahat |
| author_sort | Zahid Ifrah |
| collection | DOAJ |
| description | Cystic fibrosis is a genetic disorder caused by a mutation in the cystic fibrosis transmembrane conductance regulator (CFTR) gene that encodes an ABC transporter-class ion channel protein, resulting in sticky and thick mucus secretion which clogs the airways and leads to respiratory failure and other complications. It also results in higher chloride ions (Clˉ) in body fluids. Cystic fibrosis is generally detected using the sweat chloride test and ion exchange chromatography, which are lab restricted. Therefore, there is a dire need to develop portable assays to monitor circulatory changes (Clˉ ion detection) to detect CF at the point of care. In this work, fluorescence quenching of graphene quantum dots (GQDs) was used as a property of the optical sensor for chloride ion detection. GQDs were synthesized by varying the carbonizing temperature and time, and then their optical and fluorescence (FL) quenching was investigated upon exposure to chloride ions in comparison with different ionic species. GQDs synthesized at 160°C for 50 min were chosen as they displayed the highest fluorescence. The morphological and optical characterization confirmed the preparation of 12–15 nm GQDs, which were amorphous in nature with the peak emission observed at 462 nm when excited at 370 nm. The fluorescence quenching response of GQDs with Clˉ ions displayed linearity up to 100 mM with a correlation coefficient of 0.98 and the lowest detection limit of approximately 10 mM Clˉ ions. |
| first_indexed | 2024-12-17T06:31:39Z |
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| id | doaj.art-b12327a5f6c54761aabc9f0b4eb3faf6 |
| institution | Directory Open Access Journal |
| issn | 2296-8016 |
| language | English |
| last_indexed | 2024-12-17T06:31:39Z |
| publishDate | 2022-04-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Materials |
| spelling | doaj.art-b12327a5f6c54761aabc9f0b4eb3faf62022-12-21T22:00:08ZengFrontiers Media S.A.Frontiers in Materials2296-80162022-04-01910.3389/fmats.2022.857432857432Fluorescence Quenching of Graphene Quantum Dots by Chloride Ions: A Potential Optical Biosensor for Cystic FibrosisZahid Ifrah0Abbas Shah Rukh1Sharif Muhammad Nauman2Shahid Maryam3Ullah Rahat4Department of Industrial Biotechnology, Atta Ur Rahman School of Applied Biosciences(ASAB), National University of Sciences and Technology (NUST), Islamabad, PakistanDepartment of Industrial Biotechnology, Atta Ur Rahman School of Applied Biosciences(ASAB), National University of Sciences and Technology (NUST), Islamabad, PakistanDepartment of Industrial Biotechnology, Atta Ur Rahman School of Applied Biosciences(ASAB), National University of Sciences and Technology (NUST), Islamabad, PakistanDepartment of Industrial Biotechnology, Atta Ur Rahman School of Applied Biosciences(ASAB), National University of Sciences and Technology (NUST), Islamabad, PakistanNational Institute of Lasers and Optronics College, Pakistan Institute of Engineering and Applied Sciences, Nilore, PakistanCystic fibrosis is a genetic disorder caused by a mutation in the cystic fibrosis transmembrane conductance regulator (CFTR) gene that encodes an ABC transporter-class ion channel protein, resulting in sticky and thick mucus secretion which clogs the airways and leads to respiratory failure and other complications. It also results in higher chloride ions (Clˉ) in body fluids. Cystic fibrosis is generally detected using the sweat chloride test and ion exchange chromatography, which are lab restricted. Therefore, there is a dire need to develop portable assays to monitor circulatory changes (Clˉ ion detection) to detect CF at the point of care. In this work, fluorescence quenching of graphene quantum dots (GQDs) was used as a property of the optical sensor for chloride ion detection. GQDs were synthesized by varying the carbonizing temperature and time, and then their optical and fluorescence (FL) quenching was investigated upon exposure to chloride ions in comparison with different ionic species. GQDs synthesized at 160°C for 50 min were chosen as they displayed the highest fluorescence. The morphological and optical characterization confirmed the preparation of 12–15 nm GQDs, which were amorphous in nature with the peak emission observed at 462 nm when excited at 370 nm. The fluorescence quenching response of GQDs with Clˉ ions displayed linearity up to 100 mM with a correlation coefficient of 0.98 and the lowest detection limit of approximately 10 mM Clˉ ions.https://www.frontiersin.org/articles/10.3389/fmats.2022.857432/fullgraphene quantum dot (GQD)cystic fbrosischloride ions (Cl-)fluorescence quenchingcitric acid |
| spellingShingle | Zahid Ifrah Abbas Shah Rukh Sharif Muhammad Nauman Shahid Maryam Ullah Rahat Fluorescence Quenching of Graphene Quantum Dots by Chloride Ions: A Potential Optical Biosensor for Cystic Fibrosis Frontiers in Materials graphene quantum dot (GQD) cystic fbrosis chloride ions (Cl-) fluorescence quenching citric acid |
| title | Fluorescence Quenching of Graphene Quantum Dots by Chloride Ions: A Potential Optical Biosensor for Cystic Fibrosis |
| title_full | Fluorescence Quenching of Graphene Quantum Dots by Chloride Ions: A Potential Optical Biosensor for Cystic Fibrosis |
| title_fullStr | Fluorescence Quenching of Graphene Quantum Dots by Chloride Ions: A Potential Optical Biosensor for Cystic Fibrosis |
| title_full_unstemmed | Fluorescence Quenching of Graphene Quantum Dots by Chloride Ions: A Potential Optical Biosensor for Cystic Fibrosis |
| title_short | Fluorescence Quenching of Graphene Quantum Dots by Chloride Ions: A Potential Optical Biosensor for Cystic Fibrosis |
| title_sort | fluorescence quenching of graphene quantum dots by chloride ions a potential optical biosensor for cystic fibrosis |
| topic | graphene quantum dot (GQD) cystic fbrosis chloride ions (Cl-) fluorescence quenching citric acid |
| url | https://www.frontiersin.org/articles/10.3389/fmats.2022.857432/full |
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