MECANISMOS MOLECULARES NO HIRSUTISMO
Androgens are the main hormonal regulators of human hair growth and they are related to clinical conditions such as hirsutism. The aim of this study was to analyze the gene expression of androgen receptor (AR), type 1 and type 2 5a-reductase isoenzymes (5a R1 and 2) and type 2 17b hydroxysteroid de...
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| Format: | Article |
| Language: | English |
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Hospital de Clinicas de Porto Alegre ; Universidade Federal do Rio Grande do Sul (UFRGS)
2022-07-01
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| Series: | Clinical and Biomedical Research |
| Online Access: | https://seer.ufrgs.br/index.php/hcpa/article/view/126200 |
| _version_ | 1828149905364877312 |
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| author | Isabel Oliveira Angela D’Avila Ilma S Brum Poli Mara Spritzer |
| author_facet | Isabel Oliveira Angela D’Avila Ilma S Brum Poli Mara Spritzer |
| author_sort | Isabel Oliveira |
| collection | DOAJ |
| description |
Androgens are the main hormonal regulators of human hair growth and they are related to clinical conditions such as hirsutism. The aim of this study was to analyze the gene expression of androgen receptor (AR), type 1 and type 2 5a-reductase isoenzymes (5a R1 and 2) and type 2 17b hydroxysteroid dehydrogenase (17b-HSD 2) in plucked scalp hairs from hirsute patients and normal subjects. We studied 33 women with hirsutism [20 with polycystic ovary syndrome (PCOS), 13 with idiopathic hirsutism (IH)]; 15 control women; and 10 control men. Hirsutism was assessed by a modified Ferriman-Gallwey method. Hormonal status was assessed between days 2 and 10 of the menstrual cycle or on any day when the patients were amenorrheic. AR and enzymes mRNA levels were estimated by reverse transcriptionpolymerase chain reaction (RT-PCR). AR expression was similar in all groups. The gene expression of 5a R2 was not detected in any hair samples analyzed in this study. No differences were found on 5a R1 mRNA levels between men and normal women (0.78 ± 0.05 vs. 0.74 ± 0.06, respectively). 5a R1 gene expression in the plucked hair cells from scalp of normal women (0.85 ± 0.04), PCOS (0.78 ± 0.05) and IH (0.80 ± 0.06) was also similar. 17b-HSD2 gene expression in hirsute patients was lower (2.2±0.13 and 2.0±0.15, for PCOS and IH, respectively) than in normal women (3.1±0.17, p<0.05), and similar to men (1.8±0.22). In conclusion, these results indicate that there are no changes on 5a R1 gene expression in the plucked hair cells from scalp, related to gender or hirsutism. The lower expression of 17b-HSD2 mRNA in scalp hairs of hirsute patients suggests androgen metabolism disturbances with predominance of more potent androgens, as occurs in men.
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| first_indexed | 2024-04-11T21:36:54Z |
| format | Article |
| id | doaj.art-b123e1e617fb4187baafa97f2e1b5a46 |
| institution | Directory Open Access Journal |
| issn | 2357-9730 |
| language | English |
| last_indexed | 2024-04-11T21:36:54Z |
| publishDate | 2022-07-01 |
| publisher | Hospital de Clinicas de Porto Alegre ; Universidade Federal do Rio Grande do Sul (UFRGS) |
| record_format | Article |
| series | Clinical and Biomedical Research |
| spelling | doaj.art-b123e1e617fb4187baafa97f2e1b5a462022-12-22T04:01:43ZengHospital de Clinicas de Porto Alegre ; Universidade Federal do Rio Grande do Sul (UFRGS)Clinical and Biomedical Research2357-97302022-07-01231 - 2MECANISMOS MOLECULARES NO HIRSUTISMOIsabel Oliveira Angela D’AvilaIlma S BrumPoli Mara Spritzer Androgens are the main hormonal regulators of human hair growth and they are related to clinical conditions such as hirsutism. The aim of this study was to analyze the gene expression of androgen receptor (AR), type 1 and type 2 5a-reductase isoenzymes (5a R1 and 2) and type 2 17b hydroxysteroid dehydrogenase (17b-HSD 2) in plucked scalp hairs from hirsute patients and normal subjects. We studied 33 women with hirsutism [20 with polycystic ovary syndrome (PCOS), 13 with idiopathic hirsutism (IH)]; 15 control women; and 10 control men. Hirsutism was assessed by a modified Ferriman-Gallwey method. Hormonal status was assessed between days 2 and 10 of the menstrual cycle or on any day when the patients were amenorrheic. AR and enzymes mRNA levels were estimated by reverse transcriptionpolymerase chain reaction (RT-PCR). AR expression was similar in all groups. The gene expression of 5a R2 was not detected in any hair samples analyzed in this study. No differences were found on 5a R1 mRNA levels between men and normal women (0.78 ± 0.05 vs. 0.74 ± 0.06, respectively). 5a R1 gene expression in the plucked hair cells from scalp of normal women (0.85 ± 0.04), PCOS (0.78 ± 0.05) and IH (0.80 ± 0.06) was also similar. 17b-HSD2 gene expression in hirsute patients was lower (2.2±0.13 and 2.0±0.15, for PCOS and IH, respectively) than in normal women (3.1±0.17, p<0.05), and similar to men (1.8±0.22). In conclusion, these results indicate that there are no changes on 5a R1 gene expression in the plucked hair cells from scalp, related to gender or hirsutism. The lower expression of 17b-HSD2 mRNA in scalp hairs of hirsute patients suggests androgen metabolism disturbances with predominance of more potent androgens, as occurs in men. https://seer.ufrgs.br/index.php/hcpa/article/view/126200 |
| spellingShingle | Isabel Oliveira Angela D’Avila Ilma S Brum Poli Mara Spritzer MECANISMOS MOLECULARES NO HIRSUTISMO Clinical and Biomedical Research |
| title | MECANISMOS MOLECULARES NO HIRSUTISMO |
| title_full | MECANISMOS MOLECULARES NO HIRSUTISMO |
| title_fullStr | MECANISMOS MOLECULARES NO HIRSUTISMO |
| title_full_unstemmed | MECANISMOS MOLECULARES NO HIRSUTISMO |
| title_short | MECANISMOS MOLECULARES NO HIRSUTISMO |
| title_sort | mecanismos moleculares no hirsutismo |
| url | https://seer.ufrgs.br/index.php/hcpa/article/view/126200 |
| work_keys_str_mv | AT isabeloliveira mecanismosmolecularesnohirsutismo AT angeladavila mecanismosmolecularesnohirsutismo AT ilmasbrum mecanismosmolecularesnohirsutismo AT polimaraspritzer mecanismosmolecularesnohirsutismo |