The effect of antimicrobials on verocytotoxin bacteriophage transduction under bovine rumen fluid and broth conditions
The verocytotoxin genes in verocytotoxigenic Escherichia coli (VTEC) are carried by bacteriophages, incorporated into the bacterial genome (prophage). Antibiotics may promote phage replication and release to infect other cells (transduction), thus leading to the emergence of new VTEC strains. This s...
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Compuscript Ltd
2017-11-01
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| Series: | Irish Journal of Agricultural and Food Research |
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| Online Access: | http://www.degruyter.com/view/j/ijafr.2017.56.issue-1/ijafr-2017-0008/ijafr-2017-0008.xml?format=INT |
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| author | Nyambe S. Burgess C. Whyte P. Bolton D. |
| author_facet | Nyambe S. Burgess C. Whyte P. Bolton D. |
| author_sort | Nyambe S. |
| collection | DOAJ |
| description | The verocytotoxin genes in verocytotoxigenic Escherichia coli (VTEC) are carried by bacteriophages, incorporated into the bacterial genome (prophage). Antibiotics may promote phage replication and release to infect other cells (transduction), thus leading to the emergence of new VTEC strains. This study investigated transduction of a verocytotoxin2-encoding bacteriophage (3538(vtx2::cat)) under laboratory conditions, including the effect of antibiotic treatments. Luria-Bertani Miller broth and rumen fluid (raw and sterilised by irradiation) were inoculated with the donor (C600φ3538(Δvtx2::cat)) and recipient (E. coli C600::kanamycinR) strains (4 log10 cfu/mL) and incubated at 38°C. Antibiotic treatments (minimal inhibitory and sub-inhibitory concentrations of ampicillin, cefquinome, oxytetracycline and sodium sulfamethazine) were applied after 3 h. Samples were tested for donor, recipient, cell-free phage and transductants at times t = 0, 3, 4, 6, 27 (24 h post-antibiotic treatment) and 51 h. Free phage was detected in the untreated broth and rumen samples, as were the transductants confirmed by polymerase chain reaction. The antibiotic treatments did not significantly (P > 0.01) increase the concentrations of free phage or transductants detected. It was therefore concluded that, under laboratory conditions, the antibiotics tested did not induce bacteriophage lysis, release and infection of new bacterial cells beyond that constitutively found in the phage population. |
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| institution | Directory Open Access Journal |
| issn | 0791-6833 2009-9029 |
| language | English |
| last_indexed | 2024-03-12T07:38:40Z |
| publishDate | 2017-11-01 |
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| record_format | Article |
| series | Irish Journal of Agricultural and Food Research |
| spelling | doaj.art-b125c7e6f8604d10856fb669bdcc52e32023-09-02T21:26:13ZengCompuscript LtdIrish Journal of Agricultural and Food Research0791-68332009-90292017-11-01561778410.1515/ijafr-2017-0008ijafr-2017-0008The effect of antimicrobials on verocytotoxin bacteriophage transduction under bovine rumen fluid and broth conditionsNyambe S.0Burgess C.1Whyte P.2Bolton D.3Food Safety Department, Teagasc Food Research Centre, Ashtown, Dublin 15, IrelandFood Safety Department, Teagasc Food Research Centre, Ashtown, Dublin 15, IrelandSchool of Veterinary Medicine, University College Dublin, Belfield, Dublin 4, IrelandFood Safety Department, Teagasc Food Research Centre, Ashtown, Dublin 15, IrelandThe verocytotoxin genes in verocytotoxigenic Escherichia coli (VTEC) are carried by bacteriophages, incorporated into the bacterial genome (prophage). Antibiotics may promote phage replication and release to infect other cells (transduction), thus leading to the emergence of new VTEC strains. This study investigated transduction of a verocytotoxin2-encoding bacteriophage (3538(vtx2::cat)) under laboratory conditions, including the effect of antibiotic treatments. Luria-Bertani Miller broth and rumen fluid (raw and sterilised by irradiation) were inoculated with the donor (C600φ3538(Δvtx2::cat)) and recipient (E. coli C600::kanamycinR) strains (4 log10 cfu/mL) and incubated at 38°C. Antibiotic treatments (minimal inhibitory and sub-inhibitory concentrations of ampicillin, cefquinome, oxytetracycline and sodium sulfamethazine) were applied after 3 h. Samples were tested for donor, recipient, cell-free phage and transductants at times t = 0, 3, 4, 6, 27 (24 h post-antibiotic treatment) and 51 h. Free phage was detected in the untreated broth and rumen samples, as were the transductants confirmed by polymerase chain reaction. The antibiotic treatments did not significantly (P > 0.01) increase the concentrations of free phage or transductants detected. It was therefore concluded that, under laboratory conditions, the antibiotics tested did not induce bacteriophage lysis, release and infection of new bacterial cells beyond that constitutively found in the phage population.http://www.degruyter.com/view/j/ijafr.2017.56.issue-1/ijafr-2017-0008/ijafr-2017-0008.xml?format=INTantibioticsrumentransductionverocytotoxigenic E. colivtx2 bacteriophage |
| spellingShingle | Nyambe S. Burgess C. Whyte P. Bolton D. The effect of antimicrobials on verocytotoxin bacteriophage transduction under bovine rumen fluid and broth conditions Irish Journal of Agricultural and Food Research antibiotics rumen transduction verocytotoxigenic E. coli vtx2 bacteriophage |
| title | The effect of antimicrobials on verocytotoxin bacteriophage transduction under bovine rumen fluid and broth conditions |
| title_full | The effect of antimicrobials on verocytotoxin bacteriophage transduction under bovine rumen fluid and broth conditions |
| title_fullStr | The effect of antimicrobials on verocytotoxin bacteriophage transduction under bovine rumen fluid and broth conditions |
| title_full_unstemmed | The effect of antimicrobials on verocytotoxin bacteriophage transduction under bovine rumen fluid and broth conditions |
| title_short | The effect of antimicrobials on verocytotoxin bacteriophage transduction under bovine rumen fluid and broth conditions |
| title_sort | effect of antimicrobials on verocytotoxin bacteriophage transduction under bovine rumen fluid and broth conditions |
| topic | antibiotics rumen transduction verocytotoxigenic E. coli vtx2 bacteriophage |
| url | http://www.degruyter.com/view/j/ijafr.2017.56.issue-1/ijafr-2017-0008/ijafr-2017-0008.xml?format=INT |
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