| Summary: | In recent years, <i>Salmonella enterica</i> subsp. <i>enterica</i> serovar Mbandaka (<i>S.</i> Mbandaka) has been increasingly isolated from laying hens and shell eggs around the world. Moreover, this serovar has been identified as the causative agent of several salmonellosis outbreaks in humans. Surprisingly, little is known about the characteristics of this emerging serovar, and therefore, we investigated antimicrobial resistance, virulence, and prophage genes of six selected Brazilian strains of <i>Salmonella</i> Mbandaka using Whole Genome Sequencing (WGS). Multi-locus sequence typing revealed that the tested strains belong to Sequence Type 413 (ST413), which has been linked to recent multi-country salmonellosis outbreaks in Europe. A total of nine resistance genes were detected, and the most frequent ones were <i>aac(6′)-Iaa</i>, <i>sul1</i>, <i>qacE</i>, <i>bla</i><sub>OXA-129</sub>, <i>tet(B)</i>, and <i>aadA1</i>. A point mutation in ParC at the 57th position (threonine → serine) associated with quinolone resistance was present in all investigated genomes. A 112,960 bp IncHI2A plasmid was mapped in 4/6 strains. This plasmid harboured tetracycline (<i>tet</i>ACDR) and mercury (<i>mer</i>) resistance genes, genes contributing to conjugative transfer, and genes involved in plasmid maintenance. Most strains (four/six) carried <i>Salmonella</i> genomic island 1 (SGI1). All <i>S.</i> Mbandaka genomes carried seven pathogenicity islands (SPIs) involved in intracellular survival and virulence: SPIs 1-5, 9, and C63PI. The virulence genes <i>csgC</i>, <i>fimY</i>, <i>tcfA</i>, <i>sscA</i>, (two/six), and <i>ssaS</i> (one/six) were absent in some of the genomes; conversely, <i>fimA</i>, <i>prgH</i>, and <i>mgtC</i> were present in all of them. Five <i>Salmonella</i> bacteriophage sequences (with homology to <i>Escherichia</i> phage phiV10, <i>Enterobacteria</i> phage Fels-2, <i>Enterobacteria</i> phage HK542, <i>Enterobacteria</i> phage ST64T, <i>Salmonella</i> phage SW9) were identified, with protein counts between 31 and 54, genome lengths of 24.7 bp and 47.7 bp, and average GC content of 51.25%. In the phylogenetic analysis, the genomes of strains isolated from poultry in Brazil clustered into well-supported clades with a heterogeneous distribution, primarily associated with strains isolated from humans and food. The phylogenetic relationship of Brazilian <i>S</i>. Mbandaka suggests the presence of strains with high epidemiological significance and the potential to be linked to foodborne outbreaks. Overall, our results show that isolated strains of <i>S.</i> Mbandaka are multidrug-resistant and encode a rather conserved virulence machinery, which is an epidemiological hallmark of <i>Salmonella</i> strains that have successfully disseminated both regionally and globally.
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