Documenting the microbiome diversity and distribution in selected fleas from South Africa with an emphasis on the cat flea, Ctenocephalides f. felis.
The factors that influence parasite associated bacterial microbial diversity and the geographic distributions of bacteria are not fully understood. In an effort to gain a deeper understanding of the relationship between the bacterial diversity of Ctenocephalides fleas and host species and the extern...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Cambridge University Press
2023-09-01
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| Series: | Parasitology |
| Subjects: | |
| Online Access: | https://www.cambridge.org/core/product/identifier/S0031182023000835/type/journal_article |
| _version_ | 1797404931329622016 |
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| author | Conrad A. Matthee Anandi Bierman Boris R. Krasnov Sonja Matthee Luther van der Mescht |
| author_facet | Conrad A. Matthee Anandi Bierman Boris R. Krasnov Sonja Matthee Luther van der Mescht |
| author_sort | Conrad A. Matthee |
| collection | DOAJ |
| description | The factors that influence parasite associated bacterial microbial diversity and the geographic distributions of bacteria are not fully understood. In an effort to gain a deeper understanding of the relationship between the bacterial diversity of Ctenocephalides fleas and host species and the external environment, we conducted a metagenetic analysis of 107 flea samples collected from 8 distinct sampling sites in South Africa. Pooled DNA samples mostly comprising of 2 or 3 individuals sampled from the same host, and belonging to the same genetic cluster, were sequenced using the Ion PGM™ Hi-Q™ Kit and the Ion 316™ Chip v2. Differences were detected in the microbiome compositions between Ctenocephalides felis, Ctenocephalides canis and Ctenocephalides connatus. Although based on a small sample, C. connatus occurring on wildlife harboured a higher bacterial richness when compared to C. felis on domestic animals. Intraspecific differences in the microbial OTU diversity were detected within C. f. felis that occurred on domestic cats and dogs. Different genetic lineages of C. f. felis were similar in microbial compositions but some differences exist in the presence or absence of rare bacteria. Rickettsia and Bartonella OTU's identified in South African cat fleas differ from those identified in the USA and Australia. Intraspecific microbial compositions also differ across geographic sampling sites. Generalized dissimilarity modelling showed that temperature and humidity are potentially important environmental factors explaining the pattern obtained. |
| first_indexed | 2024-03-09T03:02:14Z |
| format | Article |
| id | doaj.art-b12a388ff18344b5899bf65e04ce94c6 |
| institution | Directory Open Access Journal |
| issn | 0031-1820 1469-8161 |
| language | English |
| last_indexed | 2024-03-09T03:02:14Z |
| publishDate | 2023-09-01 |
| publisher | Cambridge University Press |
| record_format | Article |
| series | Parasitology |
| spelling | doaj.art-b12a388ff18344b5899bf65e04ce94c62023-12-04T13:13:14ZengCambridge University PressParasitology0031-18201469-81612023-09-0115097998910.1017/S0031182023000835Documenting the microbiome diversity and distribution in selected fleas from South Africa with an emphasis on the cat flea, Ctenocephalides f. felis.Conrad A. Matthee0https://orcid.org/0000-0002-6836-069XAnandi Bierman1Boris R. Krasnov2https://orcid.org/0000-0002-0382-3331Sonja Matthee3https://orcid.org/0000-0001-7289-6262Luther van der Mescht4Department of Botany and Zoology, Evolutionary Genomics Group, Stellenbosch University, Stellenbosch, South AfricaDepartment of Conservation Ecology & Entomology, Centre for Invasion Biology, Stellenbosch University, Stellenbosch, South AfricaMitrani Department of Desert Ecology, Swiss Institute for Dryland Environmental and Energy Research, Jacob Blaustein Institutes for Desert Research, Ben-Gurion University of the Negev, Midreshet Ben-Gurion, IsraelDepartment of Conservation Ecology & Entomology, Stellenbosch University, Stellenbosch, South AfricaDepartment of Botany and Zoology, Evolutionary Genomics Group, Stellenbosch University, Stellenbosch, South AfricaThe factors that influence parasite associated bacterial microbial diversity and the geographic distributions of bacteria are not fully understood. In an effort to gain a deeper understanding of the relationship between the bacterial diversity of Ctenocephalides fleas and host species and the external environment, we conducted a metagenetic analysis of 107 flea samples collected from 8 distinct sampling sites in South Africa. Pooled DNA samples mostly comprising of 2 or 3 individuals sampled from the same host, and belonging to the same genetic cluster, were sequenced using the Ion PGM™ Hi-Q™ Kit and the Ion 316™ Chip v2. Differences were detected in the microbiome compositions between Ctenocephalides felis, Ctenocephalides canis and Ctenocephalides connatus. Although based on a small sample, C. connatus occurring on wildlife harboured a higher bacterial richness when compared to C. felis on domestic animals. Intraspecific differences in the microbial OTU diversity were detected within C. f. felis that occurred on domestic cats and dogs. Different genetic lineages of C. f. felis were similar in microbial compositions but some differences exist in the presence or absence of rare bacteria. Rickettsia and Bartonella OTU's identified in South African cat fleas differ from those identified in the USA and Australia. Intraspecific microbial compositions also differ across geographic sampling sites. Generalized dissimilarity modelling showed that temperature and humidity are potentially important environmental factors explaining the pattern obtained.https://www.cambridge.org/core/product/identifier/S0031182023000835/type/journal_articleBartonellaCtenocephalides canisCtenocephalides connatusCtenocephalides felismicrobiomeRickettsiaSouth Africa16S rRNA metagenetic |
| spellingShingle | Conrad A. Matthee Anandi Bierman Boris R. Krasnov Sonja Matthee Luther van der Mescht Documenting the microbiome diversity and distribution in selected fleas from South Africa with an emphasis on the cat flea, Ctenocephalides f. felis. Parasitology Bartonella Ctenocephalides canis Ctenocephalides connatus Ctenocephalides felis microbiome Rickettsia South Africa 16S rRNA metagenetic |
| title | Documenting the microbiome diversity and distribution in selected fleas from South Africa with an emphasis on the cat flea, Ctenocephalides f. felis. |
| title_full | Documenting the microbiome diversity and distribution in selected fleas from South Africa with an emphasis on the cat flea, Ctenocephalides f. felis. |
| title_fullStr | Documenting the microbiome diversity and distribution in selected fleas from South Africa with an emphasis on the cat flea, Ctenocephalides f. felis. |
| title_full_unstemmed | Documenting the microbiome diversity and distribution in selected fleas from South Africa with an emphasis on the cat flea, Ctenocephalides f. felis. |
| title_short | Documenting the microbiome diversity and distribution in selected fleas from South Africa with an emphasis on the cat flea, Ctenocephalides f. felis. |
| title_sort | documenting the microbiome diversity and distribution in selected fleas from south africa with an emphasis on the cat flea ctenocephalides f felis |
| topic | Bartonella Ctenocephalides canis Ctenocephalides connatus Ctenocephalides felis microbiome Rickettsia South Africa 16S rRNA metagenetic |
| url | https://www.cambridge.org/core/product/identifier/S0031182023000835/type/journal_article |
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