CD206 Expression in Induced Microglia-Like Cells From Peripheral Blood as a Surrogate Biomarker for the Specific Immune Microenvironment of Neurosurgical Diseases Including Glioma
Targeting the unique glioma immune microenvironment is a promising approach in developing breakthrough immunotherapy treatments. However, recent advances in immunotherapy, including the development of immune checkpoint inhibitors, have not improved the outcomes of patients with glioma. A way of moni...
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| Language: | English |
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Frontiers Media S.A.
2021-06-01
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| Series: | Frontiers in Immunology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fimmu.2021.670131/full |
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| author | Shunya Tanaka Masahiro Ohgidani Nobuhiro Hata Shogo Inamine Noriaki Sagata Noritoshi Shirouzu Nobutaka Mukae Satoshi O. Suzuki Hideomi Hamasaki Ryusuke Hatae Yuhei Sangatsuda Yutaka Fujioka Kosuke Takigawa Yusuke Funakoshi Toru Iwaki Masako Hosoi Koji Iihara Masahiro Mizoguchi Takahiro A. Kato |
| author_facet | Shunya Tanaka Masahiro Ohgidani Nobuhiro Hata Shogo Inamine Noriaki Sagata Noritoshi Shirouzu Nobutaka Mukae Satoshi O. Suzuki Hideomi Hamasaki Ryusuke Hatae Yuhei Sangatsuda Yutaka Fujioka Kosuke Takigawa Yusuke Funakoshi Toru Iwaki Masako Hosoi Koji Iihara Masahiro Mizoguchi Takahiro A. Kato |
| author_sort | Shunya Tanaka |
| collection | DOAJ |
| description | Targeting the unique glioma immune microenvironment is a promising approach in developing breakthrough immunotherapy treatments. However, recent advances in immunotherapy, including the development of immune checkpoint inhibitors, have not improved the outcomes of patients with glioma. A way of monitoring biological activity of immune cells in neural tissues affected by glioma should be developed to address this lack of sensitivity to immunotherapy. Thus, in this study, we sought to examine the feasibility of non-invasive monitoring of glioma-associated microglia/macrophages (GAM) by utilizing our previously developed induced microglia-like (iMG) cells. Primary microglia (pMG) were isolated from surgically obtained brain tissues of 22 patients with neurological diseases. iMG cells were produced from monocytes extracted from the patients’ peripheral blood. Quantitative reverse transcription-polymerase chain reaction (qRT-PCR) revealed a significant correlation of the expression levels of representative markers for M1 and M2 microglia phenotypes between pMG and the corresponding iMG cells in each patient (Spearman’s correlation coefficient = 0.5225, P <0.0001). Synchronous upregulation of CD206 expression levels was observed in most patients with glioma (6/9, 66.7%) and almost all patients with glioblastoma (4/5, 80%). Therefore, iMG cells can be used as a minimally invasive tool for monitoring the disease-related immunological state of GAM in various brain diseases, including glioma. CD206 upregulation detected in iMG cells can be used as a surrogate biomarker of glioma. |
| first_indexed | 2024-12-24T03:15:19Z |
| format | Article |
| id | doaj.art-b12d5139e5f54e41bfca54e07b75f532 |
| institution | Directory Open Access Journal |
| issn | 1664-3224 |
| language | English |
| last_indexed | 2024-12-24T03:15:19Z |
| publishDate | 2021-06-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Immunology |
| spelling | doaj.art-b12d5139e5f54e41bfca54e07b75f5322022-12-21T17:17:40ZengFrontiers Media S.A.Frontiers in Immunology1664-32242021-06-011210.3389/fimmu.2021.670131670131CD206 Expression in Induced Microglia-Like Cells From Peripheral Blood as a Surrogate Biomarker for the Specific Immune Microenvironment of Neurosurgical Diseases Including GliomaShunya Tanaka0Masahiro Ohgidani1Nobuhiro Hata2Shogo Inamine3Noriaki Sagata4Noritoshi Shirouzu5Nobutaka Mukae6Satoshi O. Suzuki7Hideomi Hamasaki8Ryusuke Hatae9Yuhei Sangatsuda10Yutaka Fujioka11Kosuke Takigawa12Yusuke Funakoshi13Toru Iwaki14Masako Hosoi15Koji Iihara16Masahiro Mizoguchi17Takahiro A. Kato18Department of Neurosurgery, Graduate School of Medical Sciences, Kyushu University, Fukuoka, JapanDepartment of Neuropsychiatry, Graduate School of Medical Sciences, Kyushu University, Fukuoka, JapanDepartment of Neurosurgery, Graduate School of Medical Sciences, Kyushu University, Fukuoka, JapanDepartment of Neuropsychiatry, Graduate School of Medical Sciences, Kyushu University, Fukuoka, JapanDepartment of Neuropsychiatry, Graduate School of Medical Sciences, Kyushu University, Fukuoka, JapanDepartment of Neurosurgery, Graduate School of Medical Sciences, Kyushu University, Fukuoka, JapanDepartment of Neurosurgery, Graduate School of Medical Sciences, Kyushu University, Fukuoka, JapanDepartment of Neuropathology, Graduate School of Medical Sciences, Kyushu University, Fukuoka, JapanDepartment of Neuropathology, Graduate School of Medical Sciences, Kyushu University, Fukuoka, JapanDepartment of Neurosurgery, Graduate School of Medical Sciences, Kyushu University, Fukuoka, JapanDepartment of Neurosurgery, Graduate School of Medical Sciences, Kyushu University, Fukuoka, JapanDepartment of Neurosurgery, Graduate School of Medical Sciences, Kyushu University, Fukuoka, JapanDepartment of Neurosurgery, Graduate School of Medical Sciences, Kyushu University, Fukuoka, JapanDepartment of Neurosurgery, Graduate School of Medical Sciences, Kyushu University, Fukuoka, JapanDepartment of Neuropathology, Graduate School of Medical Sciences, Kyushu University, Fukuoka, JapanDepartment of Psychosomatic Medicine, Kyushu University Hospital, Fukuoka, JapanDepartment of Neurosurgery, Graduate School of Medical Sciences, Kyushu University, Fukuoka, JapanDepartment of Neurosurgery, Graduate School of Medical Sciences, Kyushu University, Fukuoka, JapanDepartment of Neuropsychiatry, Graduate School of Medical Sciences, Kyushu University, Fukuoka, JapanTargeting the unique glioma immune microenvironment is a promising approach in developing breakthrough immunotherapy treatments. However, recent advances in immunotherapy, including the development of immune checkpoint inhibitors, have not improved the outcomes of patients with glioma. A way of monitoring biological activity of immune cells in neural tissues affected by glioma should be developed to address this lack of sensitivity to immunotherapy. Thus, in this study, we sought to examine the feasibility of non-invasive monitoring of glioma-associated microglia/macrophages (GAM) by utilizing our previously developed induced microglia-like (iMG) cells. Primary microglia (pMG) were isolated from surgically obtained brain tissues of 22 patients with neurological diseases. iMG cells were produced from monocytes extracted from the patients’ peripheral blood. Quantitative reverse transcription-polymerase chain reaction (qRT-PCR) revealed a significant correlation of the expression levels of representative markers for M1 and M2 microglia phenotypes between pMG and the corresponding iMG cells in each patient (Spearman’s correlation coefficient = 0.5225, P <0.0001). Synchronous upregulation of CD206 expression levels was observed in most patients with glioma (6/9, 66.7%) and almost all patients with glioblastoma (4/5, 80%). Therefore, iMG cells can be used as a minimally invasive tool for monitoring the disease-related immunological state of GAM in various brain diseases, including glioma. CD206 upregulation detected in iMG cells can be used as a surrogate biomarker of glioma.https://www.frontiersin.org/articles/10.3389/fimmu.2021.670131/fullmicrogliagliomaCD206surrogate biomarkerinduced microglia-like cells |
| spellingShingle | Shunya Tanaka Masahiro Ohgidani Nobuhiro Hata Shogo Inamine Noriaki Sagata Noritoshi Shirouzu Nobutaka Mukae Satoshi O. Suzuki Hideomi Hamasaki Ryusuke Hatae Yuhei Sangatsuda Yutaka Fujioka Kosuke Takigawa Yusuke Funakoshi Toru Iwaki Masako Hosoi Koji Iihara Masahiro Mizoguchi Takahiro A. Kato CD206 Expression in Induced Microglia-Like Cells From Peripheral Blood as a Surrogate Biomarker for the Specific Immune Microenvironment of Neurosurgical Diseases Including Glioma Frontiers in Immunology microglia glioma CD206 surrogate biomarker induced microglia-like cells |
| title | CD206 Expression in Induced Microglia-Like Cells From Peripheral Blood as a Surrogate Biomarker for the Specific Immune Microenvironment of Neurosurgical Diseases Including Glioma |
| title_full | CD206 Expression in Induced Microglia-Like Cells From Peripheral Blood as a Surrogate Biomarker for the Specific Immune Microenvironment of Neurosurgical Diseases Including Glioma |
| title_fullStr | CD206 Expression in Induced Microglia-Like Cells From Peripheral Blood as a Surrogate Biomarker for the Specific Immune Microenvironment of Neurosurgical Diseases Including Glioma |
| title_full_unstemmed | CD206 Expression in Induced Microglia-Like Cells From Peripheral Blood as a Surrogate Biomarker for the Specific Immune Microenvironment of Neurosurgical Diseases Including Glioma |
| title_short | CD206 Expression in Induced Microglia-Like Cells From Peripheral Blood as a Surrogate Biomarker for the Specific Immune Microenvironment of Neurosurgical Diseases Including Glioma |
| title_sort | cd206 expression in induced microglia like cells from peripheral blood as a surrogate biomarker for the specific immune microenvironment of neurosurgical diseases including glioma |
| topic | microglia glioma CD206 surrogate biomarker induced microglia-like cells |
| url | https://www.frontiersin.org/articles/10.3389/fimmu.2021.670131/full |
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