Potential of bacteriocins produced by probiotic bacteria isolated from tiger shrimp and prawns as antibacterial to Vibrio, Pseudomonas, and Aeromonas species on fish [version 1; referees: 2 approved, 1 approved with reservations, 1 not approved]

Backgrounds: Bacteriocin has been used widely in industry as a biopreservative agent. The objective of the present study was to investigate the potency of Bacteriocin isolated from tiger prawn Penaeus monodon and freshwater shrimp Macrobrachium rosenbergii as an anti-bacterial on fish. Methods: A to...

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Main Authors: Feli Feliatra, Zainal Abidin Muchlisin, Hiwan Yuda Teruna, Widya Rahmi Utamy, Nursyirwani Nursyirwani, Andi Dahliaty
Format: Article
Language:English
Published: F1000 Research Ltd 2018-03-01
Series:F1000Research
Online Access:https://f1000research.com/articles/7-415/v1
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author Feli Feliatra
Zainal Abidin Muchlisin
Hiwan Yuda Teruna
Widya Rahmi Utamy
Nursyirwani Nursyirwani
Andi Dahliaty
author_facet Feli Feliatra
Zainal Abidin Muchlisin
Hiwan Yuda Teruna
Widya Rahmi Utamy
Nursyirwani Nursyirwani
Andi Dahliaty
author_sort Feli Feliatra
collection DOAJ
description Backgrounds: Bacteriocin has been used widely in industry as a biopreservative agent. The objective of the present study was to investigate the potency of Bacteriocin isolated from tiger prawn Penaeus monodon and freshwater shrimp Macrobrachium rosenbergii as an anti-bacterial on fish. Methods: A total of ten candidates of probiotic bacteria consisted of five isolates from tiger shrimps (H1, H2, H3, H4, H5) and five isolates from freshwater prawns (W1, W2, W3, W4, W5) were evaluated. Bacteriocin wasBacteriocin was produced by centrifugation at a speed of 150 rpm and at 37 °C for 24 hours. The bacteriocin extract was purified by adding sulphate ammonium salt {(NH4) 2SO4} at 80% of the saturation level. Bacteriocin activity was determined using a diffusion method against pathogenic bacteria Vibrio alginolyticus, Aeromonas hydrophillaAeromonas hydrophilla and Pseudomonas stutzeri. Bacteriocins were analyzed usinganalyzedusing High Performance Liquid Chromatography (HPLC) and Fourier Transform Infra-Red (FTIR). The data were subjected to analysis of variance (ANOVA) and followed with Duncans multiple range test. Results: Bacteriocins produced by bacteria isolate H4 from tiger prawn indicated the highest bacteriocin activity againstbacteriocin activity against Pseudomonas stutzeri atstutzeri at the diameter of inhibition zone of 887.10 ± 409.24 mm2/mL. While isolate W2 from freshwater shrimp indicated inhibition zone of 1466.96 ± 127.62 mm2/mL. Both bacteriocins were purified by chromatography column using Sephadex LH-20. Conclusions: This finding showed that bacterial isolates H4 and W2 have the potential to produce bacteriocins which inhibit the pathogenic bacteria. FTIR analysis showed an amide group at wave number 1652cm-1 contained in the bacteriocins of isolates H4 and W2.
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spelling doaj.art-b130e087566e4b06bdeb2d6d0389cc922022-12-22T03:47:01ZengF1000 Research LtdF1000Research2046-14022018-03-01710.12688/f1000research.13958.115172Potential of bacteriocins produced by probiotic bacteria isolated from tiger shrimp and prawns as antibacterial to Vibrio, Pseudomonas, and Aeromonas species on fish [version 1; referees: 2 approved, 1 approved with reservations, 1 not approved]Feli Feliatra0Zainal Abidin Muchlisin1Hiwan Yuda Teruna2Widya Rahmi Utamy3Nursyirwani Nursyirwani4Andi Dahliaty5Marine Microbiology Laboratory, Department of Marine Science, Fisheries and Marine Sciences Faculty, University of Riau, Riau, IndonesiaDepartment of Aquaculture, Faculty of Marine and Fisheries, Syiah Kuala University, Banda Aceh, IndonesiaDeptartment of Chemistry, Faculty of Mathematics and Natural Sciences, University of Riau, Riau, IndonesiaDeptartment of Chemistry, Faculty of Mathematics and Natural Sciences, University of Riau, Riau, IndonesiaMarine Microbiology Laboratory, Department of Marine Science, Fisheries and Marine Sciences Faculty, University of Riau, Riau, IndonesiaDeptartment of Chemistry, Faculty of Mathematics and Natural Sciences, University of Riau, Riau, IndonesiaBackgrounds: Bacteriocin has been used widely in industry as a biopreservative agent. The objective of the present study was to investigate the potency of Bacteriocin isolated from tiger prawn Penaeus monodon and freshwater shrimp Macrobrachium rosenbergii as an anti-bacterial on fish. Methods: A total of ten candidates of probiotic bacteria consisted of five isolates from tiger shrimps (H1, H2, H3, H4, H5) and five isolates from freshwater prawns (W1, W2, W3, W4, W5) were evaluated. Bacteriocin wasBacteriocin was produced by centrifugation at a speed of 150 rpm and at 37 °C for 24 hours. The bacteriocin extract was purified by adding sulphate ammonium salt {(NH4) 2SO4} at 80% of the saturation level. Bacteriocin activity was determined using a diffusion method against pathogenic bacteria Vibrio alginolyticus, Aeromonas hydrophillaAeromonas hydrophilla and Pseudomonas stutzeri. Bacteriocins were analyzed usinganalyzedusing High Performance Liquid Chromatography (HPLC) and Fourier Transform Infra-Red (FTIR). The data were subjected to analysis of variance (ANOVA) and followed with Duncans multiple range test. Results: Bacteriocins produced by bacteria isolate H4 from tiger prawn indicated the highest bacteriocin activity againstbacteriocin activity against Pseudomonas stutzeri atstutzeri at the diameter of inhibition zone of 887.10 ± 409.24 mm2/mL. While isolate W2 from freshwater shrimp indicated inhibition zone of 1466.96 ± 127.62 mm2/mL. Both bacteriocins were purified by chromatography column using Sephadex LH-20. Conclusions: This finding showed that bacterial isolates H4 and W2 have the potential to produce bacteriocins which inhibit the pathogenic bacteria. FTIR analysis showed an amide group at wave number 1652cm-1 contained in the bacteriocins of isolates H4 and W2.https://f1000research.com/articles/7-415/v1
spellingShingle Feli Feliatra
Zainal Abidin Muchlisin
Hiwan Yuda Teruna
Widya Rahmi Utamy
Nursyirwani Nursyirwani
Andi Dahliaty
Potential of bacteriocins produced by probiotic bacteria isolated from tiger shrimp and prawns as antibacterial to Vibrio, Pseudomonas, and Aeromonas species on fish [version 1; referees: 2 approved, 1 approved with reservations, 1 not approved]
F1000Research
title Potential of bacteriocins produced by probiotic bacteria isolated from tiger shrimp and prawns as antibacterial to Vibrio, Pseudomonas, and Aeromonas species on fish [version 1; referees: 2 approved, 1 approved with reservations, 1 not approved]
title_full Potential of bacteriocins produced by probiotic bacteria isolated from tiger shrimp and prawns as antibacterial to Vibrio, Pseudomonas, and Aeromonas species on fish [version 1; referees: 2 approved, 1 approved with reservations, 1 not approved]
title_fullStr Potential of bacteriocins produced by probiotic bacteria isolated from tiger shrimp and prawns as antibacterial to Vibrio, Pseudomonas, and Aeromonas species on fish [version 1; referees: 2 approved, 1 approved with reservations, 1 not approved]
title_full_unstemmed Potential of bacteriocins produced by probiotic bacteria isolated from tiger shrimp and prawns as antibacterial to Vibrio, Pseudomonas, and Aeromonas species on fish [version 1; referees: 2 approved, 1 approved with reservations, 1 not approved]
title_short Potential of bacteriocins produced by probiotic bacteria isolated from tiger shrimp and prawns as antibacterial to Vibrio, Pseudomonas, and Aeromonas species on fish [version 1; referees: 2 approved, 1 approved with reservations, 1 not approved]
title_sort potential of bacteriocins produced by probiotic bacteria isolated from tiger shrimp and prawns as antibacterial to vibrio pseudomonas and aeromonas species on fish version 1 referees 2 approved 1 approved with reservations 1 not approved
url https://f1000research.com/articles/7-415/v1
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