Immunoregulatory Effects of Porcine Plasma Protein Concentrates on Rat Intestinal Epithelial Cells and Splenocytes
Serum protein concentrates have been shown to exert in vivo anti-inflammatory effects. Specific effects on different cell types and their mechanism of action remain unraveled. We aimed to characterize the immunomodulatory effect of two porcine plasma protein concentrates, spray dried serum (SDS) and...
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MDPI AG
2021-03-01
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| Online Access: | https://www.mdpi.com/2076-2615/11/3/807 |
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| author | Cristina Hernández-Chirlaque Carlos J. Aranda Borja Ocón Javier Polo Olga Martínez-Augustin Fermín Sánchez de Medina |
| author_facet | Cristina Hernández-Chirlaque Carlos J. Aranda Borja Ocón Javier Polo Olga Martínez-Augustin Fermín Sánchez de Medina |
| author_sort | Cristina Hernández-Chirlaque |
| collection | DOAJ |
| description | Serum protein concentrates have been shown to exert in vivo anti-inflammatory effects. Specific effects on different cell types and their mechanism of action remain unraveled. We aimed to characterize the immunomodulatory effect of two porcine plasma protein concentrates, spray dried serum (SDS) and an immunoglobulin concentrate (IC), currently used as animal nutritional supplements with established in vivo immunomodulatory properties. Cytokine production by the intestinal epithelial cell line IEC18 and by primary cultures of rat splenocytes was studied. The molecular pathways involved were explored with specific inhibitors and gene knockdown. Our results indicate that both products induced GROα and MCP-1 production in IEC18 cells by a MyD88/NF-κB-dependent mechanism. Inhibition of TNF production was observed in rat primary splenocyte cultures. The immunoglobulin concentrate induced IL-10 expression in primary splenocytes and lymphocytes. The effect on TNF was independent of IL-10 production or the stimulation of NF-kB, MAPKs, AKT, or RAGE. In conclusion, SDS and IC directly regulate intestinal and systemic immune response in murine intestinal epithelial cells and in T lymphocytes and monocytes. |
| first_indexed | 2024-03-10T13:16:43Z |
| format | Article |
| id | doaj.art-b13247e92ecf4d74b2fa844ac89ba2f3 |
| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-10T13:16:43Z |
| publishDate | 2021-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Animals |
| spelling | doaj.art-b13247e92ecf4d74b2fa844ac89ba2f32023-11-21T10:22:21ZengMDPI AGAnimals2076-26152021-03-0111380710.3390/ani11030807Immunoregulatory Effects of Porcine Plasma Protein Concentrates on Rat Intestinal Epithelial Cells and SplenocytesCristina Hernández-Chirlaque0Carlos J. Aranda1Borja Ocón2Javier Polo3Olga Martínez-Augustin4Fermín Sánchez de Medina5Department of Biochemistry and Molecular Biology II, School of Pharmacy, CIBERehd, Instituto de Investigación Biosanitaria ibs.GRANADA, University of Granada, 18071 Granada, SpainDepartment of Biochemistry and Molecular Biology II, School of Pharmacy, CIBERehd, Instituto de Investigación Biosanitaria ibs.GRANADA, University of Granada, 18071 Granada, SpainDepartment of Pharmacology, CIBERehd, School of Pharmacy, Instituto de Investigación Biosanitaria ibs.GRANADA, University of Granada, 18071 Granada, SpainAPC Europe S.L.U., 08403 Granollers, SpainDepartment of Biochemistry and Molecular Biology II, School of Pharmacy, CIBERehd, Instituto de Investigación Biosanitaria ibs.GRANADA, University of Granada, 18071 Granada, SpainDepartment of Pharmacology, CIBERehd, School of Pharmacy, Instituto de Investigación Biosanitaria ibs.GRANADA, University of Granada, 18071 Granada, SpainSerum protein concentrates have been shown to exert in vivo anti-inflammatory effects. Specific effects on different cell types and their mechanism of action remain unraveled. We aimed to characterize the immunomodulatory effect of two porcine plasma protein concentrates, spray dried serum (SDS) and an immunoglobulin concentrate (IC), currently used as animal nutritional supplements with established in vivo immunomodulatory properties. Cytokine production by the intestinal epithelial cell line IEC18 and by primary cultures of rat splenocytes was studied. The molecular pathways involved were explored with specific inhibitors and gene knockdown. Our results indicate that both products induced GROα and MCP-1 production in IEC18 cells by a MyD88/NF-κB-dependent mechanism. Inhibition of TNF production was observed in rat primary splenocyte cultures. The immunoglobulin concentrate induced IL-10 expression in primary splenocytes and lymphocytes. The effect on TNF was independent of IL-10 production or the stimulation of NF-kB, MAPKs, AKT, or RAGE. In conclusion, SDS and IC directly regulate intestinal and systemic immune response in murine intestinal epithelial cells and in T lymphocytes and monocytes.https://www.mdpi.com/2076-2615/11/3/807immunoglobulin concentrateserum protein concentrateToll-like receptorsTLR4Myd88 |
| spellingShingle | Cristina Hernández-Chirlaque Carlos J. Aranda Borja Ocón Javier Polo Olga Martínez-Augustin Fermín Sánchez de Medina Immunoregulatory Effects of Porcine Plasma Protein Concentrates on Rat Intestinal Epithelial Cells and Splenocytes Animals immunoglobulin concentrate serum protein concentrate Toll-like receptors TLR4 Myd88 |
| title | Immunoregulatory Effects of Porcine Plasma Protein Concentrates on Rat Intestinal Epithelial Cells and Splenocytes |
| title_full | Immunoregulatory Effects of Porcine Plasma Protein Concentrates on Rat Intestinal Epithelial Cells and Splenocytes |
| title_fullStr | Immunoregulatory Effects of Porcine Plasma Protein Concentrates on Rat Intestinal Epithelial Cells and Splenocytes |
| title_full_unstemmed | Immunoregulatory Effects of Porcine Plasma Protein Concentrates on Rat Intestinal Epithelial Cells and Splenocytes |
| title_short | Immunoregulatory Effects of Porcine Plasma Protein Concentrates on Rat Intestinal Epithelial Cells and Splenocytes |
| title_sort | immunoregulatory effects of porcine plasma protein concentrates on rat intestinal epithelial cells and splenocytes |
| topic | immunoglobulin concentrate serum protein concentrate Toll-like receptors TLR4 Myd88 |
| url | https://www.mdpi.com/2076-2615/11/3/807 |
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