Leptospirosis in Slaughterhouse Personnel: A Seroepidemiologic Study Using Microscopic Agglutination Test
Background and Objective: Meat can be contaminated by Leptospira species. This bacterial pathogen causes severe leptospirosis disease in humans and animals. The major aims of this study were to assess seroepidemiological prevalence of leptospirosis in employees of a slaughterhouse in Guilan Province...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Shahid Behehsti University of Medical Sciences
2023-12-01
|
| Series: | Applied Food Biotechnology |
| Subjects: | |
| Online Access: | https://journals.sbmu.ac.ir/afb/article/view/43527 |
| _version_ | 1797205523449249792 |
|---|---|
| author | Ali Hokmi Seyyed Saeed Eshraghi Abbas Rahimi Foroushani Gholamreza Abdollahpour Ramin Mazaheri Nezhad Fard |
| author_facet | Ali Hokmi Seyyed Saeed Eshraghi Abbas Rahimi Foroushani Gholamreza Abdollahpour Ramin Mazaheri Nezhad Fard |
| author_sort | Ali Hokmi |
| collection | DOAJ |
| description | Background and Objective: Meat can be contaminated by Leptospira species. This bacterial pathogen causes severe leptospirosis disease in humans and animals. The major aims of this study were to assess seroepidemiological prevalence of leptospirosis in employees of a slaughterhouse in Guilan Province, Iran, using microscopic agglutination test and further investigate the positive samples using nested polymerase chain reaction method.
Material and Methods: In this study, 150 employees of a slaughterhouse in Guilan Province, Iran, were participated after completing written consents and personal questionnaires. This sample size was calculated based on the mean prevalence of the pathogen in the region. After assessing sera of the participants for Leptospira antibody using microscopic agglutination test, urine samples were collected from the positive participant for further assessments using nested polymerase chain reaction.
Results and Conclusion: Based on the results, microscopic agglutination test was positive for 10.7% of the participants. However, Nested-PCR was negative for the positive microscopic agglutination tests on sera collected from the participants with antibodies against Leptospira antigens. The current results demonstrate that Leptospira can occur in asymptomatic humans in slaughterhouses and highlight the high potential of the disease transmission to humans in the province. Therefore, further extended control and prevention measures for slaughterhouse workers are recommended to guarantee the food safety.
Conflict of interest: The authors declare no conflict of interest. |
| first_indexed | 2024-04-24T08:52:28Z |
| format | Article |
| id | doaj.art-b132fac1731147b1a135de93dc40e9e7 |
| institution | Directory Open Access Journal |
| issn | 2345-5357 2423-4214 |
| language | English |
| last_indexed | 2024-04-24T08:52:28Z |
| publishDate | 2023-12-01 |
| publisher | Shahid Behehsti University of Medical Sciences |
| record_format | Article |
| series | Applied Food Biotechnology |
| spelling | doaj.art-b132fac1731147b1a135de93dc40e9e72024-04-16T09:21:00ZengShahid Behehsti University of Medical SciencesApplied Food Biotechnology2345-53572423-42142023-12-01111e5e510.22037/afb.v11i1.4352733356Leptospirosis in Slaughterhouse Personnel: A Seroepidemiologic Study Using Microscopic Agglutination TestAli Hokmi0Seyyed Saeed Eshraghi1Abbas Rahimi Foroushani2Gholamreza Abdollahpour3Ramin Mazaheri Nezhad Fard4Department of Pathobiology, School of Public Health, Tehran University of Medical Sciences, Keshavarz Blvd., Pour Sina St., Tehran, Iran, PO Box: 1417613151Department of Pathobiology, School of Public Health, Tehran University of Medical Sciences, Tehran, IranDepartment of Epidemiology and Biostatistics, School of Public Health, Tehran University of Medical Sciences, Tehran, IranDepartment of Internal Medicine, Faculty of Veterinary Medicine, University of Tehran, Tehran, IranDepartment of Pathobiology, School of Public Health, Tehran University of Medical Sciences, Tehran, IranFood Microbiology Research Center, Tehran University of Medical Sciences, Tehran, IranBackground and Objective: Meat can be contaminated by Leptospira species. This bacterial pathogen causes severe leptospirosis disease in humans and animals. The major aims of this study were to assess seroepidemiological prevalence of leptospirosis in employees of a slaughterhouse in Guilan Province, Iran, using microscopic agglutination test and further investigate the positive samples using nested polymerase chain reaction method. Material and Methods: In this study, 150 employees of a slaughterhouse in Guilan Province, Iran, were participated after completing written consents and personal questionnaires. This sample size was calculated based on the mean prevalence of the pathogen in the region. After assessing sera of the participants for Leptospira antibody using microscopic agglutination test, urine samples were collected from the positive participant for further assessments using nested polymerase chain reaction. Results and Conclusion: Based on the results, microscopic agglutination test was positive for 10.7% of the participants. However, Nested-PCR was negative for the positive microscopic agglutination tests on sera collected from the participants with antibodies against Leptospira antigens. The current results demonstrate that Leptospira can occur in asymptomatic humans in slaughterhouses and highlight the high potential of the disease transmission to humans in the province. Therefore, further extended control and prevention measures for slaughterhouse workers are recommended to guarantee the food safety. Conflict of interest: The authors declare no conflict of interest.https://journals.sbmu.ac.ir/afb/article/view/43527leptospirosisslaughterhousesmatnested-pcriran |
| spellingShingle | Ali Hokmi Seyyed Saeed Eshraghi Abbas Rahimi Foroushani Gholamreza Abdollahpour Ramin Mazaheri Nezhad Fard Leptospirosis in Slaughterhouse Personnel: A Seroepidemiologic Study Using Microscopic Agglutination Test Applied Food Biotechnology leptospirosis slaughterhouses mat nested-pcr iran |
| title | Leptospirosis in Slaughterhouse Personnel: A Seroepidemiologic Study Using Microscopic Agglutination Test |
| title_full | Leptospirosis in Slaughterhouse Personnel: A Seroepidemiologic Study Using Microscopic Agglutination Test |
| title_fullStr | Leptospirosis in Slaughterhouse Personnel: A Seroepidemiologic Study Using Microscopic Agglutination Test |
| title_full_unstemmed | Leptospirosis in Slaughterhouse Personnel: A Seroepidemiologic Study Using Microscopic Agglutination Test |
| title_short | Leptospirosis in Slaughterhouse Personnel: A Seroepidemiologic Study Using Microscopic Agglutination Test |
| title_sort | leptospirosis in slaughterhouse personnel a seroepidemiologic study using microscopic agglutination test |
| topic | leptospirosis slaughterhouses mat nested-pcr iran |
| url | https://journals.sbmu.ac.ir/afb/article/view/43527 |
| work_keys_str_mv | AT alihokmi leptospirosisinslaughterhousepersonnelaseroepidemiologicstudyusingmicroscopicagglutinationtest AT seyyedsaeedeshraghi leptospirosisinslaughterhousepersonnelaseroepidemiologicstudyusingmicroscopicagglutinationtest AT abbasrahimiforoushani leptospirosisinslaughterhousepersonnelaseroepidemiologicstudyusingmicroscopicagglutinationtest AT gholamrezaabdollahpour leptospirosisinslaughterhousepersonnelaseroepidemiologicstudyusingmicroscopicagglutinationtest AT raminmazaherinezhadfard leptospirosisinslaughterhousepersonnelaseroepidemiologicstudyusingmicroscopicagglutinationtest |