MicroRNA-137 Targets Carboxyl-terminal Binding Protein 1 in Melanoma Cell Lines
<p>Carboxyl-terminal binding protein 1 (CtBP1) is a transcriptional co-repressor that represses expression of various tumor suppressor genes. In the present study, we identified miR-137 as a potential regulator of CtBP1 expression in melanoma cells. Expression of miR-137 in melanoma cell lines...
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| Format: | Article |
| Language: | English |
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Ivyspring International Publisher
2011-01-01
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| Series: | International Journal of Biological Sciences |
| Online Access: | http://www.biolsci.org/v07p0133.htm |
| _version_ | 1828766865299603456 |
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| author | Yu Deng, Hui Deng, Feng Bi, Jing Liu, Lynn T. Bemis, David Norris, Xiao-Jing Wang, Qinghong Zhang |
| author_facet | Yu Deng, Hui Deng, Feng Bi, Jing Liu, Lynn T. Bemis, David Norris, Xiao-Jing Wang, Qinghong Zhang |
| author_sort | Yu Deng, Hui Deng, Feng Bi, Jing Liu, Lynn T. Bemis, David Norris, Xiao-Jing Wang, Qinghong Zhang |
| collection | DOAJ |
| description | <p>Carboxyl-terminal binding protein 1 (CtBP1) is a transcriptional co-repressor that represses expression of various tumor suppressor genes. In the present study, we identified miR-137 as a potential regulator of CtBP1 expression in melanoma cells. Expression of miR-137 in melanoma cell lines was found to inversely correlate with CtBP1 levels. Target Scan predicted a putative site for miR-137 within the CtBP1 3′ untranslated region (3′UTR) at nt 710-716, which is highly conserved across species. To explore the mechanism of miR-137 targeting CtBP1, we performed an Argonaute 2 (Ago2)-pull down assay, and miR-137 was identified in complex with CtBP1 mRNA. miR-137 suppressed CtBP1 3' UTR luciferase-reporter activity, and this effect was lost with deletion of the putative 3' UTR target-site. Consistent with the results of the reporter assay, ectopic expression of miR-137 reduced expression levels of CtBP1. Furthermore, expression of miR-137 increased the immediate downstream effectors of CtBP1, such as E-cadherin and Bax. The human miR-137 gene is located at chromosome 1p22, which has previously been determined to be a susceptive region for melanoma. This study suggests miR-137 may act as a tumor suppressor by directly targeting CtBP1 to inhibit epithelial-mesenchymal transition (EMT) and inducing apoptosis of melanoma cells, thus illustrating a functional link between miR-137 and CtBP1 in melanoma development.</p> |
| first_indexed | 2024-12-11T07:16:17Z |
| format | Article |
| id | doaj.art-b1334acdd76e4c269af12a576e9eb482 |
| institution | Directory Open Access Journal |
| issn | 1449-2288 |
| language | English |
| last_indexed | 2024-12-11T07:16:17Z |
| publishDate | 2011-01-01 |
| publisher | Ivyspring International Publisher |
| record_format | Article |
| series | International Journal of Biological Sciences |
| spelling | doaj.art-b1334acdd76e4c269af12a576e9eb4822022-12-22T01:16:14ZengIvyspring International PublisherInternational Journal of Biological Sciences1449-22882011-01-0171133137MicroRNA-137 Targets Carboxyl-terminal Binding Protein 1 in Melanoma Cell LinesYu Deng, Hui Deng, Feng Bi, Jing Liu, Lynn T. Bemis, David Norris, Xiao-Jing Wang, Qinghong Zhang<p>Carboxyl-terminal binding protein 1 (CtBP1) is a transcriptional co-repressor that represses expression of various tumor suppressor genes. In the present study, we identified miR-137 as a potential regulator of CtBP1 expression in melanoma cells. Expression of miR-137 in melanoma cell lines was found to inversely correlate with CtBP1 levels. Target Scan predicted a putative site for miR-137 within the CtBP1 3′ untranslated region (3′UTR) at nt 710-716, which is highly conserved across species. To explore the mechanism of miR-137 targeting CtBP1, we performed an Argonaute 2 (Ago2)-pull down assay, and miR-137 was identified in complex with CtBP1 mRNA. miR-137 suppressed CtBP1 3' UTR luciferase-reporter activity, and this effect was lost with deletion of the putative 3' UTR target-site. Consistent with the results of the reporter assay, ectopic expression of miR-137 reduced expression levels of CtBP1. Furthermore, expression of miR-137 increased the immediate downstream effectors of CtBP1, such as E-cadherin and Bax. The human miR-137 gene is located at chromosome 1p22, which has previously been determined to be a susceptive region for melanoma. This study suggests miR-137 may act as a tumor suppressor by directly targeting CtBP1 to inhibit epithelial-mesenchymal transition (EMT) and inducing apoptosis of melanoma cells, thus illustrating a functional link between miR-137 and CtBP1 in melanoma development.</p>http://www.biolsci.org/v07p0133.htm |
| spellingShingle | Yu Deng, Hui Deng, Feng Bi, Jing Liu, Lynn T. Bemis, David Norris, Xiao-Jing Wang, Qinghong Zhang MicroRNA-137 Targets Carboxyl-terminal Binding Protein 1 in Melanoma Cell Lines International Journal of Biological Sciences |
| title | MicroRNA-137 Targets Carboxyl-terminal Binding Protein 1 in Melanoma Cell Lines |
| title_full | MicroRNA-137 Targets Carboxyl-terminal Binding Protein 1 in Melanoma Cell Lines |
| title_fullStr | MicroRNA-137 Targets Carboxyl-terminal Binding Protein 1 in Melanoma Cell Lines |
| title_full_unstemmed | MicroRNA-137 Targets Carboxyl-terminal Binding Protein 1 in Melanoma Cell Lines |
| title_short | MicroRNA-137 Targets Carboxyl-terminal Binding Protein 1 in Melanoma Cell Lines |
| title_sort | microrna 137 targets carboxyl terminal binding protein 1 in melanoma cell lines |
| url | http://www.biolsci.org/v07p0133.htm |
| work_keys_str_mv | AT yudenghuidengfengbijingliulynntbemisdavidnorrisxiaojingwangqinghongzhang microrna137targetscarboxylterminalbindingprotein1inmelanomacelllines |