In Vivo Biological Behavior of Polymer Scaffolds of Natural Origin in the Bone Repair Process
Autologous bone grafts, used mainly in extensive bone loss, are considered the gold standard treatment in regenerative medicine, but still have limitations mainly in relation to the amount of bone available, donor area, morbidity and creation of additional surgical area. This fact encourages tissue...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-03-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/26/6/1598 |
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| author | Fernando Bento Cunha Karina Torres Pomini Ana Maria de Guzzi Plepis Virgínia da Conceição Amaro Martins Eduardo Gomes Machado Renato de Moraes Marcelo de Azevedo e Souza Munhoz Michela Vanessa Ribeiro Machado Marco Antonio Hungaro Duarte Murilo Priori Alcalde Daniela Vieira Buchaim Rogério Leone Buchaim Victor Augusto Ramos Fernandes Eliana de Souza Bastos Mazuqueli Pereira André Antonio Pelegrine Marcelo Rodrigues da Cunha |
| author_facet | Fernando Bento Cunha Karina Torres Pomini Ana Maria de Guzzi Plepis Virgínia da Conceição Amaro Martins Eduardo Gomes Machado Renato de Moraes Marcelo de Azevedo e Souza Munhoz Michela Vanessa Ribeiro Machado Marco Antonio Hungaro Duarte Murilo Priori Alcalde Daniela Vieira Buchaim Rogério Leone Buchaim Victor Augusto Ramos Fernandes Eliana de Souza Bastos Mazuqueli Pereira André Antonio Pelegrine Marcelo Rodrigues da Cunha |
| author_sort | Fernando Bento Cunha |
| collection | DOAJ |
| description | Autologous bone grafts, used mainly in extensive bone loss, are considered the gold standard treatment in regenerative medicine, but still have limitations mainly in relation to the amount of bone available, donor area, morbidity and creation of additional surgical area. This fact encourages tissue engineering in relation to the need to develop new biomaterials, from sources other than the individual himself. Therefore, the present study aimed to investigate the effects of an elastin and collagen matrix on the bone repair process in critical size defects in rat calvaria. The animals (Wistar rats, <i>n =</i> 30) were submitted to a surgical procedure to create the bone defect and were divided into three groups: Control Group (CG, <i>n =</i> 10), defects filled with blood clot; E24/37 Group (E24/37, <i>n =</i> 10), defects filled with bovine elastin matrix hydrolyzed for 24 h at 37 °C and C24/25 Group (C24/25, <i>n =</i> 10), defects filled with porcine collagen matrix hydrolyzed for 24 h at 25 °C. Macroscopic and radiographic analyses demonstrated the absence of inflammatory signs and infection. Microtomographical 2D and 3D images showed centripetal bone growth and restricted margins of the bone defect. Histologically, the images confirmed the pattern of bone deposition at the margins of the remaining bone and without complete closure by bone tissue. In the morphometric analysis, the groups E24/37 and C24/25 (13.68 ± 1.44; 53.20 ± 4.47, respectively) showed statistically significant differences in relation to the CG (5.86 ± 2.87). It was concluded that the matrices used as scaffolds are biocompatible and increase the formation of new bone in a critical size defect, with greater formation in the polymer derived from the intestinal serous layer of porcine origin (C24/25). |
| first_indexed | 2024-03-10T13:15:44Z |
| format | Article |
| id | doaj.art-b133ac1e4e194d07bf9f5f847ba18217 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T13:15:44Z |
| publishDate | 2021-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-b133ac1e4e194d07bf9f5f847ba182172023-11-21T10:25:38ZengMDPI AGMolecules1420-30492021-03-01266159810.3390/molecules26061598In Vivo Biological Behavior of Polymer Scaffolds of Natural Origin in the Bone Repair ProcessFernando Bento Cunha0Karina Torres Pomini1Ana Maria de Guzzi Plepis2Virgínia da Conceição Amaro Martins3Eduardo Gomes Machado4Renato de Moraes5Marcelo de Azevedo e Souza Munhoz6Michela Vanessa Ribeiro Machado7Marco Antonio Hungaro Duarte8Murilo Priori Alcalde9Daniela Vieira Buchaim10Rogério Leone Buchaim11Victor Augusto Ramos Fernandes12Eliana de Souza Bastos Mazuqueli Pereira13André Antonio Pelegrine14Marcelo Rodrigues da Cunha15Department of Morphology and Pathology, Medical College of Jundiai, Jundiaí, São Paulo 13202-550, SP, BrazilDepartment of Biological Sciences, Bauru School of Dentistry, University of São Paulo (FOB/USP), Bauru 17012-901, SP, BrazilInterunit Postgraduate Program in Bioengineering (EESC/FMRP/IQSC), University of São Paulo (USP), São Carlos 13566-590, SP, BrazilSão Carlos Institute of Chemistry, University of São Paulo, USP, São Carlos 13566-590, SP, BrazilDepartment of Morphology and Pathology, Medical College of Jundiai, Jundiaí, São Paulo 13202-550, SP, BrazilDepartment of Morphology and Pathology, Medical College of Jundiai, Jundiaí, São Paulo 13202-550, SP, BrazilDepartment of Morphology and Pathology, Medical College of Jundiai, Jundiaí, São Paulo 13202-550, SP, BrazilDepartment of Morphology and Pathology, Medical College of Jundiai, Jundiaí, São Paulo 13202-550, SP, BrazilDepartment of Dentistry, Endodontics and Dental Materials, Bauru School of Dentistry, University of São Paulo (FOB/USP), Bauru 17012-901, SP, BrazilDepartment of Health Science, Unisagrado University Center, Bauru 17011-160, SP, BrazilPostgraduate Program in Structural and Functional Interactions in Rehabilitation, University of Marilia (UNIMAR), Marília 17525-902, SP, BrazilDepartment of Biological Sciences, Bauru School of Dentistry, University of São Paulo (FOB/USP), Bauru 17012-901, SP, BrazilDepartment of Morphology and Pathology, Medical College of Jundiai, Jundiaí, São Paulo 13202-550, SP, BrazilDentistry School, University of Marilia (UNIMAR), Marília 17525-902, SP, BrazilResearch Institute, Postgraduate Program, São Leopoldo Mandic, School of Dentistry, Campinas 13045-755, SP, BrazilDepartment of Morphology and Pathology, Medical College of Jundiai, Jundiaí, São Paulo 13202-550, SP, BrazilAutologous bone grafts, used mainly in extensive bone loss, are considered the gold standard treatment in regenerative medicine, but still have limitations mainly in relation to the amount of bone available, donor area, morbidity and creation of additional surgical area. This fact encourages tissue engineering in relation to the need to develop new biomaterials, from sources other than the individual himself. Therefore, the present study aimed to investigate the effects of an elastin and collagen matrix on the bone repair process in critical size defects in rat calvaria. The animals (Wistar rats, <i>n =</i> 30) were submitted to a surgical procedure to create the bone defect and were divided into three groups: Control Group (CG, <i>n =</i> 10), defects filled with blood clot; E24/37 Group (E24/37, <i>n =</i> 10), defects filled with bovine elastin matrix hydrolyzed for 24 h at 37 °C and C24/25 Group (C24/25, <i>n =</i> 10), defects filled with porcine collagen matrix hydrolyzed for 24 h at 25 °C. Macroscopic and radiographic analyses demonstrated the absence of inflammatory signs and infection. Microtomographical 2D and 3D images showed centripetal bone growth and restricted margins of the bone defect. Histologically, the images confirmed the pattern of bone deposition at the margins of the remaining bone and without complete closure by bone tissue. In the morphometric analysis, the groups E24/37 and C24/25 (13.68 ± 1.44; 53.20 ± 4.47, respectively) showed statistically significant differences in relation to the CG (5.86 ± 2.87). It was concluded that the matrices used as scaffolds are biocompatible and increase the formation of new bone in a critical size defect, with greater formation in the polymer derived from the intestinal serous layer of porcine origin (C24/25).https://www.mdpi.com/1420-3049/26/6/1598biomaterialsbone regenerationcollagenelastinregenerative medicinetissue engineering |
| spellingShingle | Fernando Bento Cunha Karina Torres Pomini Ana Maria de Guzzi Plepis Virgínia da Conceição Amaro Martins Eduardo Gomes Machado Renato de Moraes Marcelo de Azevedo e Souza Munhoz Michela Vanessa Ribeiro Machado Marco Antonio Hungaro Duarte Murilo Priori Alcalde Daniela Vieira Buchaim Rogério Leone Buchaim Victor Augusto Ramos Fernandes Eliana de Souza Bastos Mazuqueli Pereira André Antonio Pelegrine Marcelo Rodrigues da Cunha In Vivo Biological Behavior of Polymer Scaffolds of Natural Origin in the Bone Repair Process Molecules biomaterials bone regeneration collagen elastin regenerative medicine tissue engineering |
| title | In Vivo Biological Behavior of Polymer Scaffolds of Natural Origin in the Bone Repair Process |
| title_full | In Vivo Biological Behavior of Polymer Scaffolds of Natural Origin in the Bone Repair Process |
| title_fullStr | In Vivo Biological Behavior of Polymer Scaffolds of Natural Origin in the Bone Repair Process |
| title_full_unstemmed | In Vivo Biological Behavior of Polymer Scaffolds of Natural Origin in the Bone Repair Process |
| title_short | In Vivo Biological Behavior of Polymer Scaffolds of Natural Origin in the Bone Repair Process |
| title_sort | in vivo biological behavior of polymer scaffolds of natural origin in the bone repair process |
| topic | biomaterials bone regeneration collagen elastin regenerative medicine tissue engineering |
| url | https://www.mdpi.com/1420-3049/26/6/1598 |
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