Advances in the quantification of mitochondrial function in primary human immune cells through extracellular flux analysis.
Numerous studies show that mitochondrial energy generation determines the effectiveness of immune responses. Furthermore, changes in mitochondrial function may regulate lymphocyte function in inflammatory diseases like type 2 diabetes. Analysis of lymphocyte mitochondrial function has been facilitat...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2017-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC5298256?pdf=render |
| _version_ | 1818209050327777280 |
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| author | Dequina Nicholas Elizabeth A Proctor Forum M Raval Blanche C Ip Chloe Habib Eleni Ritou Tom N Grammatopoulos Devin Steenkamp Hans Dooms Caroline M Apovian Douglas A Lauffenburger Barbara S Nikolajczyk |
| author_facet | Dequina Nicholas Elizabeth A Proctor Forum M Raval Blanche C Ip Chloe Habib Eleni Ritou Tom N Grammatopoulos Devin Steenkamp Hans Dooms Caroline M Apovian Douglas A Lauffenburger Barbara S Nikolajczyk |
| author_sort | Dequina Nicholas |
| collection | DOAJ |
| description | Numerous studies show that mitochondrial energy generation determines the effectiveness of immune responses. Furthermore, changes in mitochondrial function may regulate lymphocyte function in inflammatory diseases like type 2 diabetes. Analysis of lymphocyte mitochondrial function has been facilitated by introduction of 96-well format extracellular flux (XF96) analyzers, but the technology remains imperfect for analysis of human lymphocytes. Limitations in XF technology include the lack of practical protocols for analysis of archived human cells, and inadequate data analysis tools that require manual quality checks. Current analysis tools for XF outcomes are also unable to automatically assess data quality and delete untenable data from the relatively high number of biological replicates needed to power complex human cell studies. The objectives of work presented herein are to test the impact of common cellular manipulations on XF outcomes, and to develop and validate a new automated tool that objectively analyzes a virtually unlimited number of samples to quantitate mitochondrial function in immune cells. We present significant improvements on previous XF analyses of primary human cells that will be absolutely essential to test the prediction that changes in immune cell mitochondrial function and fuel sources support immune dysfunction in chronic inflammatory diseases like type 2 diabetes. |
| first_indexed | 2024-12-12T04:54:33Z |
| format | Article |
| id | doaj.art-b133ec24293647c7aa0023d6c157c216 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-12T04:54:33Z |
| publishDate | 2017-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-b133ec24293647c7aa0023d6c157c2162022-12-22T00:37:24ZengPublic Library of Science (PLoS)PLoS ONE1932-62032017-01-01122e017097510.1371/journal.pone.0170975Advances in the quantification of mitochondrial function in primary human immune cells through extracellular flux analysis.Dequina NicholasElizabeth A ProctorForum M RavalBlanche C IpChloe HabibEleni RitouTom N GrammatopoulosDevin SteenkampHans DoomsCaroline M ApovianDouglas A LauffenburgerBarbara S NikolajczykNumerous studies show that mitochondrial energy generation determines the effectiveness of immune responses. Furthermore, changes in mitochondrial function may regulate lymphocyte function in inflammatory diseases like type 2 diabetes. Analysis of lymphocyte mitochondrial function has been facilitated by introduction of 96-well format extracellular flux (XF96) analyzers, but the technology remains imperfect for analysis of human lymphocytes. Limitations in XF technology include the lack of practical protocols for analysis of archived human cells, and inadequate data analysis tools that require manual quality checks. Current analysis tools for XF outcomes are also unable to automatically assess data quality and delete untenable data from the relatively high number of biological replicates needed to power complex human cell studies. The objectives of work presented herein are to test the impact of common cellular manipulations on XF outcomes, and to develop and validate a new automated tool that objectively analyzes a virtually unlimited number of samples to quantitate mitochondrial function in immune cells. We present significant improvements on previous XF analyses of primary human cells that will be absolutely essential to test the prediction that changes in immune cell mitochondrial function and fuel sources support immune dysfunction in chronic inflammatory diseases like type 2 diabetes.http://europepmc.org/articles/PMC5298256?pdf=render |
| spellingShingle | Dequina Nicholas Elizabeth A Proctor Forum M Raval Blanche C Ip Chloe Habib Eleni Ritou Tom N Grammatopoulos Devin Steenkamp Hans Dooms Caroline M Apovian Douglas A Lauffenburger Barbara S Nikolajczyk Advances in the quantification of mitochondrial function in primary human immune cells through extracellular flux analysis. PLoS ONE |
| title | Advances in the quantification of mitochondrial function in primary human immune cells through extracellular flux analysis. |
| title_full | Advances in the quantification of mitochondrial function in primary human immune cells through extracellular flux analysis. |
| title_fullStr | Advances in the quantification of mitochondrial function in primary human immune cells through extracellular flux analysis. |
| title_full_unstemmed | Advances in the quantification of mitochondrial function in primary human immune cells through extracellular flux analysis. |
| title_short | Advances in the quantification of mitochondrial function in primary human immune cells through extracellular flux analysis. |
| title_sort | advances in the quantification of mitochondrial function in primary human immune cells through extracellular flux analysis |
| url | http://europepmc.org/articles/PMC5298256?pdf=render |
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