Damage to <i>Sorubim cuspicaudus</i> Sperm Cryopreserved with Ethylene Glycol
The study aimed to evaluate cryo-injury during the cryopreservation in <i>Sorubim cuspicaudus</i> sperm with ethylene glycol (EG) at different rates (6, 8, 10%). Fresh, prefrozen, and post-thawed sperm quality as motility total, velocities, mitochondria damage (Mit-d), membrane damage (M...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-01-01
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| Series: | Animals |
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| Online Access: | https://www.mdpi.com/2076-2615/13/2/235 |
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| author | Víctor Atencio-García Denia Padilla-Izquierdo Juana Robles-González Martha Prieto-Guevara Sandra Pardo-Carrasco José Espinosa-Araujo |
| author_facet | Víctor Atencio-García Denia Padilla-Izquierdo Juana Robles-González Martha Prieto-Guevara Sandra Pardo-Carrasco José Espinosa-Araujo |
| author_sort | Víctor Atencio-García |
| collection | DOAJ |
| description | The study aimed to evaluate cryo-injury during the cryopreservation in <i>Sorubim cuspicaudus</i> sperm with ethylene glycol (EG) at different rates (6, 8, 10%). Fresh, prefrozen, and post-thawed sperm quality as motility total, velocities, mitochondria damage (Mit-d), membrane damage (Mem-d), and DNA fragmentation (DNA-f), were examined. The Mit-d, Mem-d, and DNA-f were evaluated through flow cytometry. High motility (>95%) and a low percentage of Mem-d (1.0 ± 0.5%), Mit-d (1.4 ± 0.9%), and DNA-f (2.4 ± 0.8%) were recorded for fresh semen. Prefrozen semen increases in Mit-d and DNA-f were observed compared to fresh semen (<i>p</i> < 0.05). In thawed semen, increased Mit-d (2.6 to 3-fold), Mem-d (6 to 1-fold), and DNA-f (3.3 to 6.6-fold) compared to prefrozen was observed. Thawed semen showed Mit-d (34 to 37-fold), Mem-d (24.5 to 26.6-fold) and DNA-f (13 to 18.5-fold) increased high. In conclusion, the present study demonstrated that mitochondria, membrane, and DNA integrity undergo significant damage during both pre-freezing and freezing/thawing with EG inclusion percentages from 6 to 10% that affect its fertilizing capacity, which is reduced to half of that obtained with fresh semen. It is suggested that a cryoprotective solution composed of 6% EG, 6% glucose, and 5% skimmed milk powder is a useful protocol for the cryopreservation of <i>S. cuspicaudus</i> semen. |
| first_indexed | 2024-03-09T13:49:24Z |
| format | Article |
| id | doaj.art-b134eb9cdea44558bf716021a4dc56b2 |
| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-09T13:49:24Z |
| publishDate | 2023-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Animals |
| spelling | doaj.art-b134eb9cdea44558bf716021a4dc56b22023-11-30T20:53:06ZengMDPI AGAnimals2076-26152023-01-0113223510.3390/ani13020235Damage to <i>Sorubim cuspicaudus</i> Sperm Cryopreserved with Ethylene GlycolVíctor Atencio-García0Denia Padilla-Izquierdo1Juana Robles-González2Martha Prieto-Guevara3Sandra Pardo-Carrasco4José Espinosa-Araujo5CINPIC, Fishculture Research Institute, School of Veterinary Medicine and Zootechnics, Department of Aquaculture Sciences, University of Córdoba, Montería 230002, ColombiaSchool of Basic Sciences, Department of Biology, University of Córdoba, Montería 230002, ColombiaSchool of Basic Sciences, Department of Mathematics and Statistics, Universidad de Córdoba, Montería 230002, ColombiaCINPIC, Fishculture Research Institute, School of Veterinary Medicine and Zootechnics, Department of Aquaculture Sciences, University of Córdoba, Montería 230002, ColombiaSchool of Agricultural Sciences, Department of Animal Husbandry, National University of Colombia, Medellín 050034, ColombiaCINPIC, Fishculture Research Institute, School of Veterinary Medicine and Zootechnics, Department of Aquaculture Sciences, University of Córdoba, Montería 230002, ColombiaThe study aimed to evaluate cryo-injury during the cryopreservation in <i>Sorubim cuspicaudus</i> sperm with ethylene glycol (EG) at different rates (6, 8, 10%). Fresh, prefrozen, and post-thawed sperm quality as motility total, velocities, mitochondria damage (Mit-d), membrane damage (Mem-d), and DNA fragmentation (DNA-f), were examined. The Mit-d, Mem-d, and DNA-f were evaluated through flow cytometry. High motility (>95%) and a low percentage of Mem-d (1.0 ± 0.5%), Mit-d (1.4 ± 0.9%), and DNA-f (2.4 ± 0.8%) were recorded for fresh semen. Prefrozen semen increases in Mit-d and DNA-f were observed compared to fresh semen (<i>p</i> < 0.05). In thawed semen, increased Mit-d (2.6 to 3-fold), Mem-d (6 to 1-fold), and DNA-f (3.3 to 6.6-fold) compared to prefrozen was observed. Thawed semen showed Mit-d (34 to 37-fold), Mem-d (24.5 to 26.6-fold) and DNA-f (13 to 18.5-fold) increased high. In conclusion, the present study demonstrated that mitochondria, membrane, and DNA integrity undergo significant damage during both pre-freezing and freezing/thawing with EG inclusion percentages from 6 to 10% that affect its fertilizing capacity, which is reduced to half of that obtained with fresh semen. It is suggested that a cryoprotective solution composed of 6% EG, 6% glucose, and 5% skimmed milk powder is a useful protocol for the cryopreservation of <i>S. cuspicaudus</i> semen.https://www.mdpi.com/2076-2615/13/2/235cryodamageplasma membranemitochondriaDNA fragmentationreproduction |
| spellingShingle | Víctor Atencio-García Denia Padilla-Izquierdo Juana Robles-González Martha Prieto-Guevara Sandra Pardo-Carrasco José Espinosa-Araujo Damage to <i>Sorubim cuspicaudus</i> Sperm Cryopreserved with Ethylene Glycol Animals cryodamage plasma membrane mitochondria DNA fragmentation reproduction |
| title | Damage to <i>Sorubim cuspicaudus</i> Sperm Cryopreserved with Ethylene Glycol |
| title_full | Damage to <i>Sorubim cuspicaudus</i> Sperm Cryopreserved with Ethylene Glycol |
| title_fullStr | Damage to <i>Sorubim cuspicaudus</i> Sperm Cryopreserved with Ethylene Glycol |
| title_full_unstemmed | Damage to <i>Sorubim cuspicaudus</i> Sperm Cryopreserved with Ethylene Glycol |
| title_short | Damage to <i>Sorubim cuspicaudus</i> Sperm Cryopreserved with Ethylene Glycol |
| title_sort | damage to i sorubim cuspicaudus i sperm cryopreserved with ethylene glycol |
| topic | cryodamage plasma membrane mitochondria DNA fragmentation reproduction |
| url | https://www.mdpi.com/2076-2615/13/2/235 |
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