Diversity and Host Interactions among Virulent and Temperate Baltic Sea <i>Flavobacterium</i> Phages
Viruses in aquatic environments play a key role in microbial population dynamics and nutrient cycling. In particular, bacteria of the phylum <i>Bacteriodetes</i> are known to participate in recycling algal blooms. Studies of phage−host interactions involving this phylum are hen...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-01-01
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| Series: | Viruses |
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| Online Access: | https://www.mdpi.com/1999-4915/12/2/158 |
| _version_ | 1819007103115722752 |
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| author | Emelie Nilsson Oliver W. Bayfield Daniel Lundin Alfred A. Antson Karin Holmfeldt |
| author_facet | Emelie Nilsson Oliver W. Bayfield Daniel Lundin Alfred A. Antson Karin Holmfeldt |
| author_sort | Emelie Nilsson |
| collection | DOAJ |
| description | Viruses in aquatic environments play a key role in microbial population dynamics and nutrient cycling. In particular, bacteria of the phylum <i>Bacteriodetes</i> are known to participate in recycling algal blooms. Studies of phage−host interactions involving this phylum are hence important to understand the processes shaping bacterial and viral communities in the ocean as well as nutrient cycling. In this study, we isolated and sequenced three strains of flavobacteria—LMO6, LMO9, LMO8—and 38 virulent phages infecting them. These phages represent 15 species, occupying three novel genera. Additionally, one temperate phage was induced from LMO6 and was found to be competent at infecting LMO9. Functions could be predicted for a limited number of phage genes, mainly representing roles in DNA replication and virus particle formation. No metabolic genes were detected. While the phages isolated on LMO8 could infect all three bacterial strains, the LMO6 and LMO9 phages could not infect LMO8. Of the phages isolated on LMO9, several showed a host-derived reduced efficiency of plating on LMO6, potentially due to differences in DNA methyltransferase genes. Overall, these phage−host systems contribute novel genetic information to our sequence databases and present valuable tools for the study of both virulent and temperate phages. |
| first_indexed | 2024-12-21T00:19:15Z |
| format | Article |
| id | doaj.art-b1383684add24c338aaba4828d6b9988 |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-12-21T00:19:15Z |
| publishDate | 2020-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj.art-b1383684add24c338aaba4828d6b99882022-12-21T19:22:09ZengMDPI AGViruses1999-49152020-01-0112215810.3390/v12020158v12020158Diversity and Host Interactions among Virulent and Temperate Baltic Sea <i>Flavobacterium</i> PhagesEmelie Nilsson0Oliver W. Bayfield1Daniel Lundin2Alfred A. Antson3Karin Holmfeldt4Centre for Ecology and Evolution in Microbial Model Systems (EEMiS), Department of Biology and Environmental Science, Faculty of Health and Life Sciences, Linnaeus University, SE-39231 Kalmar, SwedenYork Structural Biology Laboratory, Department of Chemistry, University of York, York YO10 5DD, UKCentre for Ecology and Evolution in Microbial Model Systems (EEMiS), Department of Biology and Environmental Science, Faculty of Health and Life Sciences, Linnaeus University, SE-39231 Kalmar, SwedenYork Structural Biology Laboratory, Department of Chemistry, University of York, York YO10 5DD, UKCentre for Ecology and Evolution in Microbial Model Systems (EEMiS), Department of Biology and Environmental Science, Faculty of Health and Life Sciences, Linnaeus University, SE-39231 Kalmar, SwedenViruses in aquatic environments play a key role in microbial population dynamics and nutrient cycling. In particular, bacteria of the phylum <i>Bacteriodetes</i> are known to participate in recycling algal blooms. Studies of phage−host interactions involving this phylum are hence important to understand the processes shaping bacterial and viral communities in the ocean as well as nutrient cycling. In this study, we isolated and sequenced three strains of flavobacteria—LMO6, LMO9, LMO8—and 38 virulent phages infecting them. These phages represent 15 species, occupying three novel genera. Additionally, one temperate phage was induced from LMO6 and was found to be competent at infecting LMO9. Functions could be predicted for a limited number of phage genes, mainly representing roles in DNA replication and virus particle formation. No metabolic genes were detected. While the phages isolated on LMO8 could infect all three bacterial strains, the LMO6 and LMO9 phages could not infect LMO8. Of the phages isolated on LMO9, several showed a host-derived reduced efficiency of plating on LMO6, potentially due to differences in DNA methyltransferase genes. Overall, these phage−host systems contribute novel genetic information to our sequence databases and present valuable tools for the study of both virulent and temperate phages.https://www.mdpi.com/1999-4915/12/2/158genomicsaquaticlyticlysogenyhost range |
| spellingShingle | Emelie Nilsson Oliver W. Bayfield Daniel Lundin Alfred A. Antson Karin Holmfeldt Diversity and Host Interactions among Virulent and Temperate Baltic Sea <i>Flavobacterium</i> Phages Viruses genomics aquatic lytic lysogeny host range |
| title | Diversity and Host Interactions among Virulent and Temperate Baltic Sea <i>Flavobacterium</i> Phages |
| title_full | Diversity and Host Interactions among Virulent and Temperate Baltic Sea <i>Flavobacterium</i> Phages |
| title_fullStr | Diversity and Host Interactions among Virulent and Temperate Baltic Sea <i>Flavobacterium</i> Phages |
| title_full_unstemmed | Diversity and Host Interactions among Virulent and Temperate Baltic Sea <i>Flavobacterium</i> Phages |
| title_short | Diversity and Host Interactions among Virulent and Temperate Baltic Sea <i>Flavobacterium</i> Phages |
| title_sort | diversity and host interactions among virulent and temperate baltic sea i flavobacterium i phages |
| topic | genomics aquatic lytic lysogeny host range |
| url | https://www.mdpi.com/1999-4915/12/2/158 |
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