Early indicators of exposure to biological threat agents using host gene profiles in peripheral blood mononuclear cells
<p>Abstract</p> <p>Background</p> <p>Effective prophylaxis and treatment for infections caused by biological threat agents (BTA) rely upon early diagnosis and rapid initiation of therapy. Most methods for identifying pathogens in body fluids and tissues require that the...
| Main Authors: | , , , , , , , , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
BMC
2008-07-01
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| Series: | BMC Infectious Diseases |
| Online Access: | http://www.biomedcentral.com/1471-2334/8/104 |
| _version_ | 1818832683802820608 |
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| author | Peel Sheila Smith Leonard Paranavitana Chrysanthi Huang Xiao-Zhe Royaee Atabak Kearney Brian Cummings Christiano Mendis Chanaka Mani Sachin Dhokalia Apsara Ramamoorthy Preveen Lincoln Patrick Eker Steven Ludwig George V Neill Roger Hammamieh Rasha Das Rina Kanesa-Thasan Niranjan Hoover David Lindler Luther E Yang David Henchal Erik Jett Marti |
| author_facet | Peel Sheila Smith Leonard Paranavitana Chrysanthi Huang Xiao-Zhe Royaee Atabak Kearney Brian Cummings Christiano Mendis Chanaka Mani Sachin Dhokalia Apsara Ramamoorthy Preveen Lincoln Patrick Eker Steven Ludwig George V Neill Roger Hammamieh Rasha Das Rina Kanesa-Thasan Niranjan Hoover David Lindler Luther E Yang David Henchal Erik Jett Marti |
| author_sort | Peel Sheila |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Effective prophylaxis and treatment for infections caused by biological threat agents (BTA) rely upon early diagnosis and rapid initiation of therapy. Most methods for identifying pathogens in body fluids and tissues require that the pathogen proliferate to detectable and dangerous levels, thereby delaying diagnosis and treatment, especially during the prelatent stages when symptoms for most BTA are indistinguishable flu-like signs.</p> <p>Methods</p> <p>To detect exposures to the various pathogens more rapidly, especially during these early stages, we evaluated a suite of host responses to biological threat agents using global gene expression profiling on complementary DNA arrays.</p> <p>Results</p> <p>We found that certain gene expression patterns were unique to each pathogen and that other gene changes occurred in response to multiple agents, perhaps relating to the eventual course of illness. Nonhuman primates were exposed to some pathogens and the <it>in vitro</it> and <it>in vivo</it> findings were compared. We found major gene expression changes at the earliest times tested post exposure to aerosolized <it>B. anthracis </it>spores and 30 min post exposure to a bacterial toxin.</p> <p>Conclusion</p> <p>Host gene expression patterns have the potential to serve as diagnostic markers or predict the course of impending illness and may lead to new stage-appropriate therapeutic strategies to ameliorate the devastating effects of exposure to biothreat agents.</p> |
| first_indexed | 2024-12-19T02:06:56Z |
| format | Article |
| id | doaj.art-b139dafbe0ba43cc9af9012b59e312ad |
| institution | Directory Open Access Journal |
| issn | 1471-2334 |
| language | English |
| last_indexed | 2024-12-19T02:06:56Z |
| publishDate | 2008-07-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Infectious Diseases |
| spelling | doaj.art-b139dafbe0ba43cc9af9012b59e312ad2022-12-21T20:40:55ZengBMCBMC Infectious Diseases1471-23342008-07-018110410.1186/1471-2334-8-104Early indicators of exposure to biological threat agents using host gene profiles in peripheral blood mononuclear cellsPeel SheilaSmith LeonardParanavitana ChrysanthiHuang Xiao-ZheRoyaee AtabakKearney BrianCummings ChristianoMendis ChanakaMani SachinDhokalia ApsaraRamamoorthy PreveenLincoln PatrickEker StevenLudwig George VNeill RogerHammamieh RashaDas RinaKanesa-Thasan NiranjanHoover DavidLindler Luther EYang DavidHenchal ErikJett Marti<p>Abstract</p> <p>Background</p> <p>Effective prophylaxis and treatment for infections caused by biological threat agents (BTA) rely upon early diagnosis and rapid initiation of therapy. Most methods for identifying pathogens in body fluids and tissues require that the pathogen proliferate to detectable and dangerous levels, thereby delaying diagnosis and treatment, especially during the prelatent stages when symptoms for most BTA are indistinguishable flu-like signs.</p> <p>Methods</p> <p>To detect exposures to the various pathogens more rapidly, especially during these early stages, we evaluated a suite of host responses to biological threat agents using global gene expression profiling on complementary DNA arrays.</p> <p>Results</p> <p>We found that certain gene expression patterns were unique to each pathogen and that other gene changes occurred in response to multiple agents, perhaps relating to the eventual course of illness. Nonhuman primates were exposed to some pathogens and the <it>in vitro</it> and <it>in vivo</it> findings were compared. We found major gene expression changes at the earliest times tested post exposure to aerosolized <it>B. anthracis </it>spores and 30 min post exposure to a bacterial toxin.</p> <p>Conclusion</p> <p>Host gene expression patterns have the potential to serve as diagnostic markers or predict the course of impending illness and may lead to new stage-appropriate therapeutic strategies to ameliorate the devastating effects of exposure to biothreat agents.</p>http://www.biomedcentral.com/1471-2334/8/104 |
| spellingShingle | Peel Sheila Smith Leonard Paranavitana Chrysanthi Huang Xiao-Zhe Royaee Atabak Kearney Brian Cummings Christiano Mendis Chanaka Mani Sachin Dhokalia Apsara Ramamoorthy Preveen Lincoln Patrick Eker Steven Ludwig George V Neill Roger Hammamieh Rasha Das Rina Kanesa-Thasan Niranjan Hoover David Lindler Luther E Yang David Henchal Erik Jett Marti Early indicators of exposure to biological threat agents using host gene profiles in peripheral blood mononuclear cells BMC Infectious Diseases |
| title | Early indicators of exposure to biological threat agents using host gene profiles in peripheral blood mononuclear cells |
| title_full | Early indicators of exposure to biological threat agents using host gene profiles in peripheral blood mononuclear cells |
| title_fullStr | Early indicators of exposure to biological threat agents using host gene profiles in peripheral blood mononuclear cells |
| title_full_unstemmed | Early indicators of exposure to biological threat agents using host gene profiles in peripheral blood mononuclear cells |
| title_short | Early indicators of exposure to biological threat agents using host gene profiles in peripheral blood mononuclear cells |
| title_sort | early indicators of exposure to biological threat agents using host gene profiles in peripheral blood mononuclear cells |
| url | http://www.biomedcentral.com/1471-2334/8/104 |
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