Cationic Nanocylinders Promote Angiogenic Activities of Endothelial Cells
Polymers have been used extensively taking forms as scaffolds, patterned surface and nanoparticle for regenerative medicine applications. Angiogenesis is an essential process for successful tissue regeneration, and endothelial cell–cell interaction plays a pivotal role in regulating their tight junc...
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| Format: | Article |
| Language: | English |
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MDPI AG
2016-01-01
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| Series: | Polymers |
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| Online Access: | http://www.mdpi.com/2073-4360/8/1/15 |
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| author | Jung Bok Lee Daniel A. Balikov Jae Won Yang Ki Seok Kim Hun Kuk Park Jeong Koo Kim Il Keun Kwon Leon M. Bellan Hak-Joon Sung |
| author_facet | Jung Bok Lee Daniel A. Balikov Jae Won Yang Ki Seok Kim Hun Kuk Park Jeong Koo Kim Il Keun Kwon Leon M. Bellan Hak-Joon Sung |
| author_sort | Jung Bok Lee |
| collection | DOAJ |
| description | Polymers have been used extensively taking forms as scaffolds, patterned surface and nanoparticle for regenerative medicine applications. Angiogenesis is an essential process for successful tissue regeneration, and endothelial cell–cell interaction plays a pivotal role in regulating their tight junction formation, a hallmark of angiogenesis. Though continuous progress has been made, strategies to promote angiogenesis still rely on small molecule delivery or nuanced scaffold fabrication. As such, the recent paradigm shift from top-down to bottom-up approaches in tissue engineering necessitates development of polymer-based modular engineering tools to control angiogenesis. Here, we developed cationic nanocylinders (NCs) as inducers of cell–cell interaction and investigated their effect on angiogenic activities of human umbilical vein endothelial cells (HUVECs) in vitro. Electrospun poly (l-lactic acid) (PLLA) fibers were aminolyzed to generate positively charged NCs. The aninolyzation time was changed to produce two different aspect ratios of NCs. When HUVECs were treated with NCs, the electrostatic interaction of cationic NCs with negatively charged plasma membranes promoted migration, permeability and tubulogenesis of HUVECs compared to no treatment. This effect was more profound when the higher aspect ratio NC was used. The results indicate these NCs can be used as a new tool for the bottom-up approach to promote angiogenesis. |
| first_indexed | 2024-04-12T04:40:28Z |
| format | Article |
| id | doaj.art-b13d9ba10c9c41a6bf1bcc393b41ecc9 |
| institution | Directory Open Access Journal |
| issn | 2073-4360 |
| language | English |
| last_indexed | 2024-04-12T04:40:28Z |
| publishDate | 2016-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Polymers |
| spelling | doaj.art-b13d9ba10c9c41a6bf1bcc393b41ecc92022-12-22T03:47:41ZengMDPI AGPolymers2073-43602016-01-01811510.3390/polym8010015polym8010015Cationic Nanocylinders Promote Angiogenic Activities of Endothelial CellsJung Bok Lee0Daniel A. Balikov1Jae Won Yang2Ki Seok Kim3Hun Kuk Park4Jeong Koo Kim5Il Keun Kwon6Leon M. Bellan7Hak-Joon Sung8Department of Biomedical Engineering, Vanderbilt University, Nashville, TN 37212, USADepartment of Biomedical Engineering, Vanderbilt University, Nashville, TN 37212, USADepartment of Internal Medicine, Yonsei University of Wonju College of Medicine, Wonju, Gangwon 220-701, KoreaDepartment of Medical Engineering, College of Medicine, Kyung Hee University, Seoul 130-701, KoreaDepartment of Medical Engineering, College of Medicine, Kyung Hee University, Seoul 130-701, KoreaDepartment of Biomedical Engineering, Vanderbilt University, Nashville, TN 37212, USADepartment of Maxillofacial Biomedical Engineering and Institute of Oral Biology, School of Dentistry, Kyung Hee University, Seoul 130-701, KoreaDepartment of Biomedical Engineering, Vanderbilt University, Nashville, TN 37212, USADepartment of Biomedical Engineering, Vanderbilt University, Nashville, TN 37212, USAPolymers have been used extensively taking forms as scaffolds, patterned surface and nanoparticle for regenerative medicine applications. Angiogenesis is an essential process for successful tissue regeneration, and endothelial cell–cell interaction plays a pivotal role in regulating their tight junction formation, a hallmark of angiogenesis. Though continuous progress has been made, strategies to promote angiogenesis still rely on small molecule delivery or nuanced scaffold fabrication. As such, the recent paradigm shift from top-down to bottom-up approaches in tissue engineering necessitates development of polymer-based modular engineering tools to control angiogenesis. Here, we developed cationic nanocylinders (NCs) as inducers of cell–cell interaction and investigated their effect on angiogenic activities of human umbilical vein endothelial cells (HUVECs) in vitro. Electrospun poly (l-lactic acid) (PLLA) fibers were aminolyzed to generate positively charged NCs. The aninolyzation time was changed to produce two different aspect ratios of NCs. When HUVECs were treated with NCs, the electrostatic interaction of cationic NCs with negatively charged plasma membranes promoted migration, permeability and tubulogenesis of HUVECs compared to no treatment. This effect was more profound when the higher aspect ratio NC was used. The results indicate these NCs can be used as a new tool for the bottom-up approach to promote angiogenesis.http://www.mdpi.com/2073-4360/8/1/15cationic nanocylindercell–cell interactionendothelial cellsmigrationtubulogenesis |
| spellingShingle | Jung Bok Lee Daniel A. Balikov Jae Won Yang Ki Seok Kim Hun Kuk Park Jeong Koo Kim Il Keun Kwon Leon M. Bellan Hak-Joon Sung Cationic Nanocylinders Promote Angiogenic Activities of Endothelial Cells Polymers cationic nanocylinder cell–cell interaction endothelial cells migration tubulogenesis |
| title | Cationic Nanocylinders Promote Angiogenic Activities of Endothelial Cells |
| title_full | Cationic Nanocylinders Promote Angiogenic Activities of Endothelial Cells |
| title_fullStr | Cationic Nanocylinders Promote Angiogenic Activities of Endothelial Cells |
| title_full_unstemmed | Cationic Nanocylinders Promote Angiogenic Activities of Endothelial Cells |
| title_short | Cationic Nanocylinders Promote Angiogenic Activities of Endothelial Cells |
| title_sort | cationic nanocylinders promote angiogenic activities of endothelial cells |
| topic | cationic nanocylinder cell–cell interaction endothelial cells migration tubulogenesis |
| url | http://www.mdpi.com/2073-4360/8/1/15 |
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