Actin Is Required for Cellular Development and Virulence of <italic toggle="yes">Botrytis cinerea</italic> via the Mediation of Secretory Proteins
ABSTRACT Actin is a vital component of the cytoskeleton of living cells and is involved in several complex processes. However, its functions in plant-pathogenic fungi are largely unknown. In this paper, we found that deletion of the Botrytis cinerea actin gene bcactA reduced growth and sporulation o...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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American Society for Microbiology
2020-02-01
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| Series: | mSystems |
| Subjects: | |
| Online Access: | https://journals.asm.org/doi/10.1128/mSystems.00732-19 |
| _version_ | 1818345716868710400 |
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| author | Hua Li Zhanquan Zhang Guozheng Qin Chang He Boqiang Li Shiping Tian |
| author_facet | Hua Li Zhanquan Zhang Guozheng Qin Chang He Boqiang Li Shiping Tian |
| author_sort | Hua Li |
| collection | DOAJ |
| description | ABSTRACT Actin is a vital component of the cytoskeleton of living cells and is involved in several complex processes. However, its functions in plant-pathogenic fungi are largely unknown. In this paper, we found that deletion of the Botrytis cinerea actin gene bcactA reduced growth and sporulation of B. cinerea and lowered virulence. Based on iTRAQ (isobaric tags for relative and absolute quantification)-based proteomic analysis, we compared changes of the secretome in ΔbcactA and wild-type strains. A total of 40 proteins exhibited significant differences in abundance in ΔbcactA mutants compared with the wild type. These proteins included 11 down-accumulated cell wall-degrading enzymes (CWDEs). Among them, two CWDEs, cellobiohydrolase (BcCBH) and β-endoglucanase (BcEG), were found to contribute to the virulence of B. cinerea, indicating that bcactA plays a crucial role in regulating the secretion of extracellular virulence factors. These findings unveil previously unknown functions of BcactA to mediate the virulence of B. cinerea and provide new mechanistic insights into the role of BcactA in the complex pathogenesis of B. cinerea. IMPORTANCE The cytoskeleton is an important network that exists in cells of all domains of life. In eukaryotic cells, actin is a vital component of the cytoskeleton. Here, we report that BcactA, an actin protein in B. cinerea, can affect the growth, sporulation, and virulence of B. cinerea. Furthermore, iTRAQ-based proteomic analysis showed that BcactA affects the abundance of 40 extracellular proteins, including 11 down-accumulated CWDEs. Among them, two CWDEs, cellobiohydrolase (BcCBH) and β-endoglucanase (BcEG), contributed to the virulence of B. cinerea, indicating that bcactA plays a crucial role in regulating extracellular virulence factors. These findings unveil previously unknown functions of BcactA in mediating growth, sporulation, and virulence of B. cinerea. |
| first_indexed | 2024-12-13T17:06:48Z |
| format | Article |
| id | doaj.art-b13eb476a60a4584866cd8a712c962f7 |
| institution | Directory Open Access Journal |
| issn | 2379-5077 |
| language | English |
| last_indexed | 2024-12-13T17:06:48Z |
| publishDate | 2020-02-01 |
| publisher | American Society for Microbiology |
| record_format | Article |
| series | mSystems |
| spelling | doaj.art-b13eb476a60a4584866cd8a712c962f72022-12-21T23:37:40ZengAmerican Society for MicrobiologymSystems2379-50772020-02-015110.1128/mSystems.00732-19Actin Is Required for Cellular Development and Virulence of <italic toggle="yes">Botrytis cinerea</italic> via the Mediation of Secretory ProteinsHua Li0Zhanquan Zhang1Guozheng Qin2Chang He3Boqiang Li4Shiping Tian5Key Laboratory of Plant Resources, Institute of Botany, The Innovative Academy of Seed Design, Chinese Academy of Sciences, Beijing, ChinaKey Laboratory of Plant Resources, Institute of Botany, The Innovative Academy of Seed Design, Chinese Academy of Sciences, Beijing, ChinaKey Laboratory of Plant Resources, Institute of Botany, The Innovative Academy of Seed Design, Chinese Academy of Sciences, Beijing, ChinaKey Laboratory of Plant Resources, Institute of Botany, The Innovative Academy of Seed Design, Chinese Academy of Sciences, Beijing, ChinaKey Laboratory of Plant Resources, Institute of Botany, The Innovative Academy of Seed Design, Chinese Academy of Sciences, Beijing, ChinaKey Laboratory of Plant Resources, Institute of Botany, The Innovative Academy of Seed Design, Chinese Academy of Sciences, Beijing, ChinaABSTRACT Actin is a vital component of the cytoskeleton of living cells and is involved in several complex processes. However, its functions in plant-pathogenic fungi are largely unknown. In this paper, we found that deletion of the Botrytis cinerea actin gene bcactA reduced growth and sporulation of B. cinerea and lowered virulence. Based on iTRAQ (isobaric tags for relative and absolute quantification)-based proteomic analysis, we compared changes of the secretome in ΔbcactA and wild-type strains. A total of 40 proteins exhibited significant differences in abundance in ΔbcactA mutants compared with the wild type. These proteins included 11 down-accumulated cell wall-degrading enzymes (CWDEs). Among them, two CWDEs, cellobiohydrolase (BcCBH) and β-endoglucanase (BcEG), were found to contribute to the virulence of B. cinerea, indicating that bcactA plays a crucial role in regulating the secretion of extracellular virulence factors. These findings unveil previously unknown functions of BcactA to mediate the virulence of B. cinerea and provide new mechanistic insights into the role of BcactA in the complex pathogenesis of B. cinerea. IMPORTANCE The cytoskeleton is an important network that exists in cells of all domains of life. In eukaryotic cells, actin is a vital component of the cytoskeleton. Here, we report that BcactA, an actin protein in B. cinerea, can affect the growth, sporulation, and virulence of B. cinerea. Furthermore, iTRAQ-based proteomic analysis showed that BcactA affects the abundance of 40 extracellular proteins, including 11 down-accumulated CWDEs. Among them, two CWDEs, cellobiohydrolase (BcCBH) and β-endoglucanase (BcEG), contributed to the virulence of B. cinerea, indicating that bcactA plays a crucial role in regulating extracellular virulence factors. These findings unveil previously unknown functions of BcactA in mediating growth, sporulation, and virulence of B. cinerea.https://journals.asm.org/doi/10.1128/mSystems.00732-19actinBotrytis cinereapathogenesissecretome |
| spellingShingle | Hua Li Zhanquan Zhang Guozheng Qin Chang He Boqiang Li Shiping Tian Actin Is Required for Cellular Development and Virulence of <italic toggle="yes">Botrytis cinerea</italic> via the Mediation of Secretory Proteins mSystems actin Botrytis cinerea pathogenesis secretome |
| title | Actin Is Required for Cellular Development and Virulence of <italic toggle="yes">Botrytis cinerea</italic> via the Mediation of Secretory Proteins |
| title_full | Actin Is Required for Cellular Development and Virulence of <italic toggle="yes">Botrytis cinerea</italic> via the Mediation of Secretory Proteins |
| title_fullStr | Actin Is Required for Cellular Development and Virulence of <italic toggle="yes">Botrytis cinerea</italic> via the Mediation of Secretory Proteins |
| title_full_unstemmed | Actin Is Required for Cellular Development and Virulence of <italic toggle="yes">Botrytis cinerea</italic> via the Mediation of Secretory Proteins |
| title_short | Actin Is Required for Cellular Development and Virulence of <italic toggle="yes">Botrytis cinerea</italic> via the Mediation of Secretory Proteins |
| title_sort | actin is required for cellular development and virulence of italic toggle yes botrytis cinerea italic via the mediation of secretory proteins |
| topic | actin Botrytis cinerea pathogenesis secretome |
| url | https://journals.asm.org/doi/10.1128/mSystems.00732-19 |
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