Brachyspira hyodysenteriae and B. pilosicoli Proteins Recognized by Sera of Challenged Pigs
The spirochetes Brachyspira hyodysenteriae and B. pilosicoli are pig intestinal pathogens that are the causative agents of swine dysentery (SD) and porcine intestinal spirochaetosis (PIS), respectively. Although some inactivated bacterin and recombinant vaccines have been explored as prophylactic tr...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2017-05-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | http://journal.frontiersin.org/article/10.3389/fmicb.2017.00723/full |
| _version_ | 1828926460098772992 |
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| author | Vanessa Casas Vanessa Casas Arantza Rodríguez-Asiain Roberto Pinto-Llorente Santiago Vadillo Montserrat Carrascal Joaquin Abian Joaquin Abian |
| author_facet | Vanessa Casas Vanessa Casas Arantza Rodríguez-Asiain Roberto Pinto-Llorente Santiago Vadillo Montserrat Carrascal Joaquin Abian Joaquin Abian |
| author_sort | Vanessa Casas |
| collection | DOAJ |
| description | The spirochetes Brachyspira hyodysenteriae and B. pilosicoli are pig intestinal pathogens that are the causative agents of swine dysentery (SD) and porcine intestinal spirochaetosis (PIS), respectively. Although some inactivated bacterin and recombinant vaccines have been explored as prophylactic treatments against these species, no effective vaccine is yet available. Immunoproteomics approaches hold the potential for the identification of new, suitable candidates for subunit vaccines against SD and PIS. These strategies take into account the gene products actually expressed and present in the cells, and thus susceptible of being targets of immune recognition. In this context, we have analyzed the immunogenic pattern of two B. pilosicoli porcine isolates (the Spanish farm isolate OLA9 and the commercial P43/6/78 strain) and one B. hyodysenteriae isolate (the Spanish farm V1). The proteins from the Brachyspira lysates were fractionated by preparative isoelectric focusing, and the fractions were analyzed by Western blot with hyperimmune sera from challenged pigs. Of the 28 challenge-specific immunoreactive bands detected, 21 were identified as single proteins by MS, while the other 7 were shown to contain several major proteins. None of these proteins were detected in the control immunoreactive bands. The proteins identified included 11 from B. hyodysenteriae and 28 from the two B. pilosicoli strains. Eight proteins were common to the B. pilosicoli strains (i.e., elongation factor G, aspartyl-tRNA synthase, biotin lipoyl, TmpB outer membrane protein, flagellar protein FlaA, enolase, PEPCK, and VspD), and enolase and PEPCK were common to both species. Many of the identified proteins were flagellar proteins or predicted to be located on the cell surface and some of them had been previously described as antigenic or as bacterial virulence factors. Here we report on the identification and semiquantitative data of these immunoreactive proteins which constitute a unique antigen collection from these bacteria. |
| first_indexed | 2024-12-13T23:34:14Z |
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| id | doaj.art-b13f7ac59e0c4bfba12dc2c7362c9c9d |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-13T23:34:14Z |
| publishDate | 2017-05-01 |
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| series | Frontiers in Microbiology |
| spelling | doaj.art-b13f7ac59e0c4bfba12dc2c7362c9c9d2022-12-21T23:27:21ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2017-05-01810.3389/fmicb.2017.00723256463Brachyspira hyodysenteriae and B. pilosicoli Proteins Recognized by Sera of Challenged PigsVanessa Casas0Vanessa Casas1Arantza Rodríguez-Asiain2Roberto Pinto-Llorente3Santiago Vadillo4Montserrat Carrascal5Joaquin Abian6Joaquin Abian7CSIC/UAB Proteomics Laboratory, IIBB-CSIC, IDIBAPSBarcelona, SpainFaculty of Medicine, Autonomous University of BarcelonaBarcelona, SpainCSIC/UAB Proteomics Laboratory, IIBB-CSIC, IDIBAPSBarcelona, SpainCSIC/UAB Proteomics Laboratory, IIBB-CSIC, IDIBAPSBarcelona, SpainDepartamento Sanidad Animal, Facultad de Veterinaria, Universidad de ExtremaduraCáceres, SpainCSIC/UAB Proteomics Laboratory, IIBB-CSIC, IDIBAPSBarcelona, SpainCSIC/UAB Proteomics Laboratory, IIBB-CSIC, IDIBAPSBarcelona, SpainFaculty of Medicine, Autonomous University of BarcelonaBarcelona, SpainThe spirochetes Brachyspira hyodysenteriae and B. pilosicoli are pig intestinal pathogens that are the causative agents of swine dysentery (SD) and porcine intestinal spirochaetosis (PIS), respectively. Although some inactivated bacterin and recombinant vaccines have been explored as prophylactic treatments against these species, no effective vaccine is yet available. Immunoproteomics approaches hold the potential for the identification of new, suitable candidates for subunit vaccines against SD and PIS. These strategies take into account the gene products actually expressed and present in the cells, and thus susceptible of being targets of immune recognition. In this context, we have analyzed the immunogenic pattern of two B. pilosicoli porcine isolates (the Spanish farm isolate OLA9 and the commercial P43/6/78 strain) and one B. hyodysenteriae isolate (the Spanish farm V1). The proteins from the Brachyspira lysates were fractionated by preparative isoelectric focusing, and the fractions were analyzed by Western blot with hyperimmune sera from challenged pigs. Of the 28 challenge-specific immunoreactive bands detected, 21 were identified as single proteins by MS, while the other 7 were shown to contain several major proteins. None of these proteins were detected in the control immunoreactive bands. The proteins identified included 11 from B. hyodysenteriae and 28 from the two B. pilosicoli strains. Eight proteins were common to the B. pilosicoli strains (i.e., elongation factor G, aspartyl-tRNA synthase, biotin lipoyl, TmpB outer membrane protein, flagellar protein FlaA, enolase, PEPCK, and VspD), and enolase and PEPCK were common to both species. Many of the identified proteins were flagellar proteins or predicted to be located on the cell surface and some of them had been previously described as antigenic or as bacterial virulence factors. Here we report on the identification and semiquantitative data of these immunoreactive proteins which constitute a unique antigen collection from these bacteria.http://journal.frontiersin.org/article/10.3389/fmicb.2017.00723/fullBrachyspiravaccineimmunoblotmass spectrometryantigenflagellar protein |
| spellingShingle | Vanessa Casas Vanessa Casas Arantza Rodríguez-Asiain Roberto Pinto-Llorente Santiago Vadillo Montserrat Carrascal Joaquin Abian Joaquin Abian Brachyspira hyodysenteriae and B. pilosicoli Proteins Recognized by Sera of Challenged Pigs Frontiers in Microbiology Brachyspira vaccine immunoblot mass spectrometry antigen flagellar protein |
| title | Brachyspira hyodysenteriae and B. pilosicoli Proteins Recognized by Sera of Challenged Pigs |
| title_full | Brachyspira hyodysenteriae and B. pilosicoli Proteins Recognized by Sera of Challenged Pigs |
| title_fullStr | Brachyspira hyodysenteriae and B. pilosicoli Proteins Recognized by Sera of Challenged Pigs |
| title_full_unstemmed | Brachyspira hyodysenteriae and B. pilosicoli Proteins Recognized by Sera of Challenged Pigs |
| title_short | Brachyspira hyodysenteriae and B. pilosicoli Proteins Recognized by Sera of Challenged Pigs |
| title_sort | brachyspira hyodysenteriae and b pilosicoli proteins recognized by sera of challenged pigs |
| topic | Brachyspira vaccine immunoblot mass spectrometry antigen flagellar protein |
| url | http://journal.frontiersin.org/article/10.3389/fmicb.2017.00723/full |
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