Efficient Biodiesel Production Catalyzed by Nanobioconjugate of Lipase from <i>Pseudomonas fluorescens</i>
The Amano lipase from <i>Pseudomonas fluorescens</i> (L-AK) was covalently immobilized on various carbon nanomaterials (functionalized single-walled carbon nanotubes and graphene oxide) and tested for biodiesel production. Using the most active lipase preparation (covalently immobilized...
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| Format: | Article |
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MDPI AG
2020-02-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/25/3/651 |
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| author | Judith-Hajnal Bartha-Vári Mădălina Elena Moisă László Csaba Bencze Florin-Dan Irimie Csaba Paizs Monica Ioana Toșa |
| author_facet | Judith-Hajnal Bartha-Vári Mădălina Elena Moisă László Csaba Bencze Florin-Dan Irimie Csaba Paizs Monica Ioana Toșa |
| author_sort | Judith-Hajnal Bartha-Vári |
| collection | DOAJ |
| description | The Amano lipase from <i>Pseudomonas fluorescens</i> (L-AK) was covalently immobilized on various carbon nanomaterials (functionalized single-walled carbon nanotubes and graphene oxide) and tested for biodiesel production. Using the most active lipase preparation (covalently immobilized L-AK on SwCNT<sub>NH2</sub> derivatized with glycerol diglycidyl ether) under optimal conditions, quasi-complete conversion (>99%) of sunflower oil was obtained after only 4 h reaction time. Moreover, the biocatalyst maintained more than 99% of its initial activity in the batch system after multiple recycling experiments. |
| first_indexed | 2024-12-11T07:14:01Z |
| format | Article |
| id | doaj.art-b14150872ca0495382e12dae30cd165d |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-12-11T07:14:01Z |
| publishDate | 2020-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-b14150872ca0495382e12dae30cd165d2022-12-22T01:16:17ZengMDPI AGMolecules1420-30492020-02-0125365110.3390/molecules25030651molecules25030651Efficient Biodiesel Production Catalyzed by Nanobioconjugate of Lipase from <i>Pseudomonas fluorescens</i>Judith-Hajnal Bartha-Vári0Mădălina Elena Moisă1László Csaba Bencze2Florin-Dan Irimie3Csaba Paizs4Monica Ioana Toșa5Babeș-Bolyai University, Biocatalysis and Biotransformation Research Center, Arany János 11, 400028 Cluj-Napoca, RomaniaBabeș-Bolyai University, Biocatalysis and Biotransformation Research Center, Arany János 11, 400028 Cluj-Napoca, RomaniaBabeș-Bolyai University, Biocatalysis and Biotransformation Research Center, Arany János 11, 400028 Cluj-Napoca, RomaniaBabeș-Bolyai University, Biocatalysis and Biotransformation Research Center, Arany János 11, 400028 Cluj-Napoca, RomaniaBabeș-Bolyai University, Biocatalysis and Biotransformation Research Center, Arany János 11, 400028 Cluj-Napoca, RomaniaBabeș-Bolyai University, Biocatalysis and Biotransformation Research Center, Arany János 11, 400028 Cluj-Napoca, RomaniaThe Amano lipase from <i>Pseudomonas fluorescens</i> (L-AK) was covalently immobilized on various carbon nanomaterials (functionalized single-walled carbon nanotubes and graphene oxide) and tested for biodiesel production. Using the most active lipase preparation (covalently immobilized L-AK on SwCNT<sub>NH2</sub> derivatized with glycerol diglycidyl ether) under optimal conditions, quasi-complete conversion (>99%) of sunflower oil was obtained after only 4 h reaction time. Moreover, the biocatalyst maintained more than 99% of its initial activity in the batch system after multiple recycling experiments.https://www.mdpi.com/1420-3049/25/3/651lipase from <i>pseudomonas fluorescens</i>single-walled carbon nanotubesbiodiesel production |
| spellingShingle | Judith-Hajnal Bartha-Vári Mădălina Elena Moisă László Csaba Bencze Florin-Dan Irimie Csaba Paizs Monica Ioana Toșa Efficient Biodiesel Production Catalyzed by Nanobioconjugate of Lipase from <i>Pseudomonas fluorescens</i> Molecules lipase from <i>pseudomonas fluorescens</i> single-walled carbon nanotubes biodiesel production |
| title | Efficient Biodiesel Production Catalyzed by Nanobioconjugate of Lipase from <i>Pseudomonas fluorescens</i> |
| title_full | Efficient Biodiesel Production Catalyzed by Nanobioconjugate of Lipase from <i>Pseudomonas fluorescens</i> |
| title_fullStr | Efficient Biodiesel Production Catalyzed by Nanobioconjugate of Lipase from <i>Pseudomonas fluorescens</i> |
| title_full_unstemmed | Efficient Biodiesel Production Catalyzed by Nanobioconjugate of Lipase from <i>Pseudomonas fluorescens</i> |
| title_short | Efficient Biodiesel Production Catalyzed by Nanobioconjugate of Lipase from <i>Pseudomonas fluorescens</i> |
| title_sort | efficient biodiesel production catalyzed by nanobioconjugate of lipase from i pseudomonas fluorescens i |
| topic | lipase from <i>pseudomonas fluorescens</i> single-walled carbon nanotubes biodiesel production |
| url | https://www.mdpi.com/1420-3049/25/3/651 |
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