Extraction and recovery technique for myxozoan parasites from the Piaractus mesopotamicus kidney embedded in paraffin
Tissues fixed and embedded in paraffin for histopathological studies keep their cell characteristics. There are several protocols for extracting genetic material from tissue embedded in paraffin, but there is no protocol for material aimed at the direct identification of parasites. The lack of techn...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Universidade Federal de Santa Catarina
2013-09-01
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| Series: | Biotemas |
| Subjects: | |
| Online Access: | https://periodicos.ufsc.br/index.php/biotemas/article/view/29701 |
| _version_ | 1818251646502699008 |
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| author | Wilson Gomez Manrique Mayra Araguaia Pereira Figueiredo Gustavo da Silva Claudiano Maurício Laterça Martins Flávio Ruas Moraes |
| author_facet | Wilson Gomez Manrique Mayra Araguaia Pereira Figueiredo Gustavo da Silva Claudiano Maurício Laterça Martins Flávio Ruas Moraes |
| author_sort | Wilson Gomez Manrique |
| collection | DOAJ |
| description | Tissues fixed and embedded in paraffin for histopathological studies keep their cell characteristics. There are several protocols for extracting genetic material from tissue embedded in paraffin, but there is no protocol for material aimed at the direct identification of parasites. The lack of techniques which describe the recovery of parasites from tissue embedded in paraffin has led us to test a technique for recovering myxosporean parasites found in Piaractus mesopotamicus kidney fragments embedded in paraffin, for a rapid, direct, and economic identification. Once the excess paraffin was removed from the kidney fragment, this was deparaffinized in xylene, hydrated in 70% alcohol, placed in an Eppendorf tube containing 70% alcohol, and left under vigorous and constant agitation in a vortex until the tissue was disintegrated. The precipitated material was mixed with the 70% alcohol and 20 µL were collected for preparing the smears, which were stained with Giemsa. Myxobolus sp. spores at many developmental stages were observed by light microscopy. The technique has proved to be useful for recovering myxosporean parasites from tissue embedded in paraffin and it constitutes an effective tool for prevalence studies when the myxosporean parasites are not detected in fresh mounts. |
| first_indexed | 2024-12-12T16:11:36Z |
| format | Article |
| id | doaj.art-b14358e9aab0489eb11114fc61f3580a |
| institution | Directory Open Access Journal |
| issn | 0103-1643 2175-7925 |
| language | English |
| last_indexed | 2024-12-12T16:11:36Z |
| publishDate | 2013-09-01 |
| publisher | Universidade Federal de Santa Catarina |
| record_format | Article |
| series | Biotemas |
| spelling | doaj.art-b14358e9aab0489eb11114fc61f3580a2022-12-22T00:19:11ZengUniversidade Federal de Santa CatarinaBiotemas0103-16432175-79252013-09-0126426326610.5007/2175-7925.2013v26n4p26320643Extraction and recovery technique for myxozoan parasites from the Piaractus mesopotamicus kidney embedded in paraffinWilson Gomez ManriqueMayra Araguaia Pereira FigueiredoGustavo da Silva ClaudianoMaurício Laterça MartinsFlávio Ruas MoraesTissues fixed and embedded in paraffin for histopathological studies keep their cell characteristics. There are several protocols for extracting genetic material from tissue embedded in paraffin, but there is no protocol for material aimed at the direct identification of parasites. The lack of techniques which describe the recovery of parasites from tissue embedded in paraffin has led us to test a technique for recovering myxosporean parasites found in Piaractus mesopotamicus kidney fragments embedded in paraffin, for a rapid, direct, and economic identification. Once the excess paraffin was removed from the kidney fragment, this was deparaffinized in xylene, hydrated in 70% alcohol, placed in an Eppendorf tube containing 70% alcohol, and left under vigorous and constant agitation in a vortex until the tissue was disintegrated. The precipitated material was mixed with the 70% alcohol and 20 µL were collected for preparing the smears, which were stained with Giemsa. Myxobolus sp. spores at many developmental stages were observed by light microscopy. The technique has proved to be useful for recovering myxosporean parasites from tissue embedded in paraffin and it constitutes an effective tool for prevalence studies when the myxosporean parasites are not detected in fresh mounts.https://periodicos.ufsc.br/index.php/biotemas/article/view/29701Extraction techniqueMyxozoaPacuParasitology |
| spellingShingle | Wilson Gomez Manrique Mayra Araguaia Pereira Figueiredo Gustavo da Silva Claudiano Maurício Laterça Martins Flávio Ruas Moraes Extraction and recovery technique for myxozoan parasites from the Piaractus mesopotamicus kidney embedded in paraffin Biotemas Extraction technique Myxozoa Pacu Parasitology |
| title | Extraction and recovery technique for myxozoan parasites from the Piaractus mesopotamicus kidney embedded in paraffin |
| title_full | Extraction and recovery technique for myxozoan parasites from the Piaractus mesopotamicus kidney embedded in paraffin |
| title_fullStr | Extraction and recovery technique for myxozoan parasites from the Piaractus mesopotamicus kidney embedded in paraffin |
| title_full_unstemmed | Extraction and recovery technique for myxozoan parasites from the Piaractus mesopotamicus kidney embedded in paraffin |
| title_short | Extraction and recovery technique for myxozoan parasites from the Piaractus mesopotamicus kidney embedded in paraffin |
| title_sort | extraction and recovery technique for myxozoan parasites from the piaractus mesopotamicus kidney embedded in paraffin |
| topic | Extraction technique Myxozoa Pacu Parasitology |
| url | https://periodicos.ufsc.br/index.php/biotemas/article/view/29701 |
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