Expression analysis of hormonal pathways and defense associated genes in gamma-rays mutagenized wheat genotypes against combined stresses of spot blotch and terminal heat
Wheat (Tritium aestivum L.) productivity is severely hampered by various pathogens and changing climatic conditions. Spot blotch and terminal heat stress are the major constraints of wheat production in the eastern Gangetic plains of India. To identify novel breeding sources and to understand underl...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2022-01-01
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| Series: | Current Plant Biology |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2214662821000396 |
| _version_ | 1819174650884653056 |
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| author | G Mahendra Singh SrinathaReddy S Gaurav Sharma Suman Bakshi Uttam Kumar Pradeep Bhati Sanjay J. Jambhulkar Ramesh Chand Arun K. Joshi Vinod K. Mishra Sandeep Sharma |
| author_facet | G Mahendra Singh SrinathaReddy S Gaurav Sharma Suman Bakshi Uttam Kumar Pradeep Bhati Sanjay J. Jambhulkar Ramesh Chand Arun K. Joshi Vinod K. Mishra Sandeep Sharma |
| author_sort | G Mahendra Singh |
| collection | DOAJ |
| description | Wheat (Tritium aestivum L.) productivity is severely hampered by various pathogens and changing climatic conditions. Spot blotch and terminal heat stress are the major constraints of wheat production in the eastern Gangetic plains of India. To identify novel breeding sources and to understand underlying resistance mechanisms, forty-four gamma rays mutagenized wheat genotypes, derived from three different parents were screened under favourable agro-ecological conditions for spot blotch and terminal heat stress. Ten mutants showed reduced spot blotch infection calculated based on Area Under Disease Progress Curve (AUDPC), than their respective parents. The mutant TAW41 had the least infection (AUDPC: 354.32), significantly lower than its parent HD2967 (AUDPC: 675.51) and other checks. TAW41 also had a higher Normalized Difference Vegetation Index (NDVI) and chlorophyll content than the parent. Gene expression analysis of TAW41 showed differential accumulation of transcripts involved in hormonal pathways (Salicylic acid, Jasmonic acid, and ethylene) and other defense-associated genes, indicating that TAW41 might have unique resistance mechanism that facilitates this genotype to perform better against the combined stress of spot blotch and terminal heat. Hence, mutant TAW41 has been identified as a novel source of resistance that could be exploited in wheat improvement programmes to enhance tolerance to spot blotch and terminal heat stress. |
| first_indexed | 2024-12-22T20:42:21Z |
| format | Article |
| id | doaj.art-b143a912c8db43c7bcb66d98abaa00d5 |
| institution | Directory Open Access Journal |
| issn | 2214-6628 |
| language | English |
| last_indexed | 2024-12-22T20:42:21Z |
| publishDate | 2022-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Current Plant Biology |
| spelling | doaj.art-b143a912c8db43c7bcb66d98abaa00d52022-12-21T18:13:18ZengElsevierCurrent Plant Biology2214-66282022-01-0129100234Expression analysis of hormonal pathways and defense associated genes in gamma-rays mutagenized wheat genotypes against combined stresses of spot blotch and terminal heatG Mahendra Singh0SrinathaReddy S1Gaurav Sharma2Suman Bakshi3Uttam Kumar4Pradeep Bhati5Sanjay J. Jambhulkar6Ramesh Chand7Arun K. Joshi8Vinod K. Mishra9Sandeep Sharma10Department of Genetics and Plant Breeding, Institute of Agricultural Sciences, Banaras Hindu University, Varanasi 221005, IndiaDepartment of Genetics and Plant Breeding, Institute of Agricultural Sciences, Banaras Hindu University, Varanasi 221005, IndiaDepartment of Genetics and Plant Breeding, Institute of Agricultural Sciences, Banaras Hindu University, Varanasi 221005, IndiaNuclear Agriculture and Biotechnology Division, BARC, Mumbai 400085, IndiaBorlaug Institute for South Asia, Ludhiana, IndiaBorlaug Institute for South Asia, Ludhiana, IndiaNuclear Agriculture and Biotechnology Division, BARC, Mumbai 400085, IndiaDepartment of Plant Pathology, Institute of Agricultural Sciences, Banaras Hindu University, Varanasi 221005, IndiaBorlaug Institute for South Asia, Ludhiana, India; International Maize and Wheat Improvement Center (CIMMYT), New Delhi, IndiaDepartment of Genetics and Plant Breeding, Institute of Agricultural Sciences, Banaras Hindu University, Varanasi 221005, India; Corresponding authors.Department of Genetics and Plant Breeding, Institute of Agricultural Sciences, Banaras Hindu University, Varanasi 221005, India; Corresponding authors.Wheat (Tritium aestivum L.) productivity is severely hampered by various pathogens and changing climatic conditions. Spot blotch and terminal heat stress are the major constraints of wheat production in the eastern Gangetic plains of India. To identify novel breeding sources and to understand underlying resistance mechanisms, forty-four gamma rays mutagenized wheat genotypes, derived from three different parents were screened under favourable agro-ecological conditions for spot blotch and terminal heat stress. Ten mutants showed reduced spot blotch infection calculated based on Area Under Disease Progress Curve (AUDPC), than their respective parents. The mutant TAW41 had the least infection (AUDPC: 354.32), significantly lower than its parent HD2967 (AUDPC: 675.51) and other checks. TAW41 also had a higher Normalized Difference Vegetation Index (NDVI) and chlorophyll content than the parent. Gene expression analysis of TAW41 showed differential accumulation of transcripts involved in hormonal pathways (Salicylic acid, Jasmonic acid, and ethylene) and other defense-associated genes, indicating that TAW41 might have unique resistance mechanism that facilitates this genotype to perform better against the combined stress of spot blotch and terminal heat. Hence, mutant TAW41 has been identified as a novel source of resistance that could be exploited in wheat improvement programmes to enhance tolerance to spot blotch and terminal heat stress.http://www.sciencedirect.com/science/article/pii/S2214662821000396WheatMutantSpot blotchTerminal heatGene expressionHormones |
| spellingShingle | G Mahendra Singh SrinathaReddy S Gaurav Sharma Suman Bakshi Uttam Kumar Pradeep Bhati Sanjay J. Jambhulkar Ramesh Chand Arun K. Joshi Vinod K. Mishra Sandeep Sharma Expression analysis of hormonal pathways and defense associated genes in gamma-rays mutagenized wheat genotypes against combined stresses of spot blotch and terminal heat Current Plant Biology Wheat Mutant Spot blotch Terminal heat Gene expression Hormones |
| title | Expression analysis of hormonal pathways and defense associated genes in gamma-rays mutagenized wheat genotypes against combined stresses of spot blotch and terminal heat |
| title_full | Expression analysis of hormonal pathways and defense associated genes in gamma-rays mutagenized wheat genotypes against combined stresses of spot blotch and terminal heat |
| title_fullStr | Expression analysis of hormonal pathways and defense associated genes in gamma-rays mutagenized wheat genotypes against combined stresses of spot blotch and terminal heat |
| title_full_unstemmed | Expression analysis of hormonal pathways and defense associated genes in gamma-rays mutagenized wheat genotypes against combined stresses of spot blotch and terminal heat |
| title_short | Expression analysis of hormonal pathways and defense associated genes in gamma-rays mutagenized wheat genotypes against combined stresses of spot blotch and terminal heat |
| title_sort | expression analysis of hormonal pathways and defense associated genes in gamma rays mutagenized wheat genotypes against combined stresses of spot blotch and terminal heat |
| topic | Wheat Mutant Spot blotch Terminal heat Gene expression Hormones |
| url | http://www.sciencedirect.com/science/article/pii/S2214662821000396 |
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