Sevoflurane Induces a Cyclophilin D-Dependent Decrease of Neural Progenitor Cells Migration
Clinical studies have suggested that repeated exposure to anesthesia and surgery at a young age may increase the risk of cognitive impairment. Our previous research has shown that sevoflurane can affect neurogenesis and cognitive function in young animals by altering cyclophilin D (CypD) levels and...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-04-01
|
| Series: | International Journal of Molecular Sciences |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1422-0067/24/7/6746 |
| _version_ | 1797607758455898112 |
|---|---|
| author | Pan Lu Feng Liang Yuanlin Dong Zhongcong Xie Yiying Zhang |
| author_facet | Pan Lu Feng Liang Yuanlin Dong Zhongcong Xie Yiying Zhang |
| author_sort | Pan Lu |
| collection | DOAJ |
| description | Clinical studies have suggested that repeated exposure to anesthesia and surgery at a young age may increase the risk of cognitive impairment. Our previous research has shown that sevoflurane can affect neurogenesis and cognitive function in young animals by altering cyclophilin D (CypD) levels and mitochondrial function. Neural progenitor cells (NPCs) migration is associated with cognitive function in developing brains. However, it is unclear whether sevoflurane can regulate NPCs migration via changes in CypD. To address this question, we treated NPCs harvested from wild-type (WT) and CypD knockout (KO) mice and young WT and CypD KO mice with sevoflurane. We used immunofluorescence staining, wound healing assay, transwell assay, mass spectrometry, and Western blot to assess the effects of sevoflurane on CypD, reactive oxygen species (ROS), doublecortin levels, and NPCs migration. We showed that sevoflurane increased levels of CypD and ROS, decreased levels of doublecortin, and reduced migration of NPCs harvested from WT mice in vitro and in WT young mice. KO of CypD attenuated these effects, suggesting that a sevoflurane-induced decrease in NPCs migration is dependent on CypD. Our findings have established a system for future studies aimed at exploring the impacts of sevoflurane anesthesia on the impairment of NPCs migration. |
| first_indexed | 2024-03-11T05:34:06Z |
| format | Article |
| id | doaj.art-b1444b25d66949708244d897f6323b06 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-11T05:34:06Z |
| publishDate | 2023-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-b1444b25d66949708244d897f6323b062023-11-17T16:55:28ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-04-01247674610.3390/ijms24076746Sevoflurane Induces a Cyclophilin D-Dependent Decrease of Neural Progenitor Cells MigrationPan Lu0Feng Liang1Yuanlin Dong2Zhongcong Xie3Yiying Zhang4Department of Anesthesia, The Second Affiliated Hospital of Xi’an Jiaotong University, Xi’an 710004, ChinaDepartment of Anesthesia, Critical Care and Pain Medicine, Massachusetts General Hospital and Harvard Medical School, Charlestown, MA 02129, USADepartment of Anesthesia, Critical Care and Pain Medicine, Massachusetts General Hospital and Harvard Medical School, Charlestown, MA 02129, USADepartment of Anesthesia, Critical Care and Pain Medicine, Massachusetts General Hospital and Harvard Medical School, Charlestown, MA 02129, USADepartment of Anesthesia, Critical Care and Pain Medicine, Massachusetts General Hospital and Harvard Medical School, Charlestown, MA 02129, USAClinical studies have suggested that repeated exposure to anesthesia and surgery at a young age may increase the risk of cognitive impairment. Our previous research has shown that sevoflurane can affect neurogenesis and cognitive function in young animals by altering cyclophilin D (CypD) levels and mitochondrial function. Neural progenitor cells (NPCs) migration is associated with cognitive function in developing brains. However, it is unclear whether sevoflurane can regulate NPCs migration via changes in CypD. To address this question, we treated NPCs harvested from wild-type (WT) and CypD knockout (KO) mice and young WT and CypD KO mice with sevoflurane. We used immunofluorescence staining, wound healing assay, transwell assay, mass spectrometry, and Western blot to assess the effects of sevoflurane on CypD, reactive oxygen species (ROS), doublecortin levels, and NPCs migration. We showed that sevoflurane increased levels of CypD and ROS, decreased levels of doublecortin, and reduced migration of NPCs harvested from WT mice in vitro and in WT young mice. KO of CypD attenuated these effects, suggesting that a sevoflurane-induced decrease in NPCs migration is dependent on CypD. Our findings have established a system for future studies aimed at exploring the impacts of sevoflurane anesthesia on the impairment of NPCs migration.https://www.mdpi.com/1422-0067/24/7/6746sevofluranecyclophilin Dneural progenitor cellsmigrationdoublecortin |
| spellingShingle | Pan Lu Feng Liang Yuanlin Dong Zhongcong Xie Yiying Zhang Sevoflurane Induces a Cyclophilin D-Dependent Decrease of Neural Progenitor Cells Migration International Journal of Molecular Sciences sevoflurane cyclophilin D neural progenitor cells migration doublecortin |
| title | Sevoflurane Induces a Cyclophilin D-Dependent Decrease of Neural Progenitor Cells Migration |
| title_full | Sevoflurane Induces a Cyclophilin D-Dependent Decrease of Neural Progenitor Cells Migration |
| title_fullStr | Sevoflurane Induces a Cyclophilin D-Dependent Decrease of Neural Progenitor Cells Migration |
| title_full_unstemmed | Sevoflurane Induces a Cyclophilin D-Dependent Decrease of Neural Progenitor Cells Migration |
| title_short | Sevoflurane Induces a Cyclophilin D-Dependent Decrease of Neural Progenitor Cells Migration |
| title_sort | sevoflurane induces a cyclophilin d dependent decrease of neural progenitor cells migration |
| topic | sevoflurane cyclophilin D neural progenitor cells migration doublecortin |
| url | https://www.mdpi.com/1422-0067/24/7/6746 |
| work_keys_str_mv | AT panlu sevofluraneinducesacyclophilinddependentdecreaseofneuralprogenitorcellsmigration AT fengliang sevofluraneinducesacyclophilinddependentdecreaseofneuralprogenitorcellsmigration AT yuanlindong sevofluraneinducesacyclophilinddependentdecreaseofneuralprogenitorcellsmigration AT zhongcongxie sevofluraneinducesacyclophilinddependentdecreaseofneuralprogenitorcellsmigration AT yiyingzhang sevofluraneinducesacyclophilinddependentdecreaseofneuralprogenitorcellsmigration |