Antimicrobial activities of the Streptomyces ceolicolor strain AOB KF977550 isolated from a tropical estuary
The aim of this study was to screen for important antibiotic producing species of the genus Streptomyces from a tropical estuary. Five bacterial strains were isolated from the Lagos lagoon and identified by 16S rDNA gene sequencing as Streptomyces albogriseolus, S. aureus, S. coelicolor, S. albus, a...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2017-11-01
|
| Series: | Journal of Taibah University for Science |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1658365517300304 |
| _version_ | 1811285381323161600 |
|---|---|
| author | Bamidele T. Odumosu Olanike M. Buraimoh Chiamaka J. Okeke James O. Ogah Frederick C. Michel, Jr. |
| author_facet | Bamidele T. Odumosu Olanike M. Buraimoh Chiamaka J. Okeke James O. Ogah Frederick C. Michel, Jr. |
| author_sort | Bamidele T. Odumosu |
| collection | DOAJ |
| description | The aim of this study was to screen for important antibiotic producing species of the genus Streptomyces from a tropical estuary. Five bacterial strains were isolated from the Lagos lagoon and identified by 16S rDNA gene sequencing as Streptomyces albogriseolus, S. aureus, S. coelicolor, S. albus, and S. pseudogriseolus. Ethyl acetate extracts of Streptomyces spp. fermented broths were evaluated against laboratory strains of MRSA Methicillin-resistant Staphyloccus aureus (MRSA) 144 m, Bacillus coagulans UL001, and Escherichia coli as well as the standard strains Klebsiella pneumonia ATCC 8308, Gardnerella vaginalis ATCC 27853 and Salmonella typhi ATCC 13311 using the well diffusion method. The presence of secondary metabolites was determined and analysed using gas chromatography-mass spectrometry (GC-MS). A broad spectrum of activity was only observed for S. coelicolor on all of the tested bacteria except S. typhi, ant GC-MS analysis revealed the presence of 16 secondary metabolites with relevant antibiotic properties. The result of this study suggest that Lagos Lagoon is a potential source and reservoir of novel antibiotics. Keywords: Streptomyces, Antibiotics, Resistance, Secondary Metabolites |
| first_indexed | 2024-04-13T02:43:20Z |
| format | Article |
| id | doaj.art-b144762a69e542b89f8c0f504f859147 |
| institution | Directory Open Access Journal |
| issn | 1658-3655 |
| language | English |
| last_indexed | 2024-04-13T02:43:20Z |
| publishDate | 2017-11-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Journal of Taibah University for Science |
| spelling | doaj.art-b144762a69e542b89f8c0f504f8591472022-12-22T03:06:08ZengTaylor & Francis GroupJournal of Taibah University for Science1658-36552017-11-01116836841Antimicrobial activities of the Streptomyces ceolicolor strain AOB KF977550 isolated from a tropical estuaryBamidele T. Odumosu0Olanike M. Buraimoh1Chiamaka J. Okeke2James O. Ogah3Frederick C. Michel, Jr.4Department of Microbiology, University of Lagos, Akoka-Yaba, Lagos State, Nigeria; Corresponding author.Department of Microbiology, University of Lagos, Akoka-Yaba, Lagos State, NigeriaDepartment of Microbiology, University of Lagos, Akoka-Yaba, Lagos State, NigeriaDepartment of Microbiology, University of Lagos, Akoka-Yaba, Lagos State, NigeriaDepartment of Food, Agricultural and Biological Engineering, The Ohio State University, Wooster, OH 44691, USAThe aim of this study was to screen for important antibiotic producing species of the genus Streptomyces from a tropical estuary. Five bacterial strains were isolated from the Lagos lagoon and identified by 16S rDNA gene sequencing as Streptomyces albogriseolus, S. aureus, S. coelicolor, S. albus, and S. pseudogriseolus. Ethyl acetate extracts of Streptomyces spp. fermented broths were evaluated against laboratory strains of MRSA Methicillin-resistant Staphyloccus aureus (MRSA) 144 m, Bacillus coagulans UL001, and Escherichia coli as well as the standard strains Klebsiella pneumonia ATCC 8308, Gardnerella vaginalis ATCC 27853 and Salmonella typhi ATCC 13311 using the well diffusion method. The presence of secondary metabolites was determined and analysed using gas chromatography-mass spectrometry (GC-MS). A broad spectrum of activity was only observed for S. coelicolor on all of the tested bacteria except S. typhi, ant GC-MS analysis revealed the presence of 16 secondary metabolites with relevant antibiotic properties. The result of this study suggest that Lagos Lagoon is a potential source and reservoir of novel antibiotics. Keywords: Streptomyces, Antibiotics, Resistance, Secondary Metaboliteshttp://www.sciencedirect.com/science/article/pii/S1658365517300304 |
| spellingShingle | Bamidele T. Odumosu Olanike M. Buraimoh Chiamaka J. Okeke James O. Ogah Frederick C. Michel, Jr. Antimicrobial activities of the Streptomyces ceolicolor strain AOB KF977550 isolated from a tropical estuary Journal of Taibah University for Science |
| title | Antimicrobial activities of the Streptomyces ceolicolor strain AOB KF977550 isolated from a tropical estuary |
| title_full | Antimicrobial activities of the Streptomyces ceolicolor strain AOB KF977550 isolated from a tropical estuary |
| title_fullStr | Antimicrobial activities of the Streptomyces ceolicolor strain AOB KF977550 isolated from a tropical estuary |
| title_full_unstemmed | Antimicrobial activities of the Streptomyces ceolicolor strain AOB KF977550 isolated from a tropical estuary |
| title_short | Antimicrobial activities of the Streptomyces ceolicolor strain AOB KF977550 isolated from a tropical estuary |
| title_sort | antimicrobial activities of the streptomyces ceolicolor strain aob kf977550 isolated from a tropical estuary |
| url | http://www.sciencedirect.com/science/article/pii/S1658365517300304 |
| work_keys_str_mv | AT bamideletodumosu antimicrobialactivitiesofthestreptomycesceolicolorstrainaobkf977550isolatedfromatropicalestuary AT olanikemburaimoh antimicrobialactivitiesofthestreptomycesceolicolorstrainaobkf977550isolatedfromatropicalestuary AT chiamakajokeke antimicrobialactivitiesofthestreptomycesceolicolorstrainaobkf977550isolatedfromatropicalestuary AT jamesoogah antimicrobialactivitiesofthestreptomycesceolicolorstrainaobkf977550isolatedfromatropicalestuary AT frederickcmicheljr antimicrobialactivitiesofthestreptomycesceolicolorstrainaobkf977550isolatedfromatropicalestuary |