Genus-Wide Microsatellite Primers for the Goldenrods (<i>Solidago</i>; Asteraceae)
Premise of the study: Microsatellite primers were developed for studies of polyploid evolution, ecological genetics, conservation genetics, and species delimitation in the genus <i>Solidago</i>. Methods and Results: Illumina sequencing of a shotgun library from <i>S. gigantea</...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2014-04-01
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| Series: | Applications in Plant Sciences |
| Subjects: | |
| Online Access: | http://www.bioone.org/doi/full/10.3732/apps.1300093 |
| _version_ | 1828156392070971392 |
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| author | James B. Beck John C. Semple Justin M. Brull Stacey L. Lance Mai M. Phillips Sara B. Hoot Gretchen A. Meyer |
| author_facet | James B. Beck John C. Semple Justin M. Brull Stacey L. Lance Mai M. Phillips Sara B. Hoot Gretchen A. Meyer |
| author_sort | James B. Beck |
| collection | DOAJ |
| description | Premise of the study: Microsatellite primers were developed for studies of polyploid evolution, ecological genetics, conservation genetics, and species delimitation in the genus <i>Solidago</i>.
Methods and Results: Illumina sequencing of a shotgun library from <i>S. gigantea</i> identified ca. 1900 putative single-copy loci. Fourteen loci were subsequently shown to be amplifiable, single-copy, and variable in a broad range of <i>Solidago</i> species.
Conclusions: The utility of these markers both across the genus and in herbarium specimens of a wide age range will facilitate numerous inter- and intraspecific studies in the ca. 120 <i>Solidago</i> species. |
| first_indexed | 2024-04-11T23:10:03Z |
| format | Article |
| id | doaj.art-b14648cfa72e48bc82f1122d27361e7b |
| institution | Directory Open Access Journal |
| issn | 2168-0450 |
| language | English |
| last_indexed | 2024-04-11T23:10:03Z |
| publishDate | 2014-04-01 |
| publisher | Wiley |
| record_format | Article |
| series | Applications in Plant Sciences |
| spelling | doaj.art-b14648cfa72e48bc82f1122d27361e7b2022-12-22T03:57:53ZengWileyApplications in Plant Sciences2168-04502014-04-0124130009310.3732/apps.1300093Genus-Wide Microsatellite Primers for the Goldenrods (<i>Solidago</i>; Asteraceae)James B. Beck0John C. Semple1Justin M. Brull2Stacey L. Lance3Mai M. Phillips4Sara B. Hoot5Gretchen A. Meyer6Department of Biological Sciences, Wichita State University, 537 Hubbard Hall, Wichita, Kansas 67260 USADepartment of Biology, University of Waterloo, Waterloo, Ontario NL2 3G1, CanadaDepartment of Biological Sciences, Wichita State University, 537 Hubbard Hall, Wichita, Kansas 67260 USASavannah River Ecology Laboratory, University of Georgia, Aiken, South Carolina 29802 USAConservation and Environmental Science Program, University of Wisconsin–Milwaukee, 3209 N. Maryland Ave., Milwaukee, Wisconsin 53201 USADepartment of Biological Sciences, University of Wisconsin–Milwaukee, 3209 N. Maryland Ave., Milwaukee, Wisconsin 53201 USAField Station, University of Wisconsin–Milwaukee, 3095 Blue Goose Road, Saukville, Wisconsin 53080 USAPremise of the study: Microsatellite primers were developed for studies of polyploid evolution, ecological genetics, conservation genetics, and species delimitation in the genus <i>Solidago</i>. Methods and Results: Illumina sequencing of a shotgun library from <i>S. gigantea</i> identified ca. 1900 putative single-copy loci. Fourteen loci were subsequently shown to be amplifiable, single-copy, and variable in a broad range of <i>Solidago</i> species. Conclusions: The utility of these markers both across the genus and in herbarium specimens of a wide age range will facilitate numerous inter- and intraspecific studies in the ca. 120 <i>Solidago</i> species.http://www.bioone.org/doi/full/10.3732/apps.1300093AsteraceaeIllumina sequencingpolyploidysimple sequence repeat (SSR) markersSolidago |
| spellingShingle | James B. Beck John C. Semple Justin M. Brull Stacey L. Lance Mai M. Phillips Sara B. Hoot Gretchen A. Meyer Genus-Wide Microsatellite Primers for the Goldenrods (<i>Solidago</i>; Asteraceae) Applications in Plant Sciences Asteraceae Illumina sequencing polyploidy simple sequence repeat (SSR) markers Solidago |
| title | Genus-Wide Microsatellite Primers for the Goldenrods (<i>Solidago</i>; Asteraceae) |
| title_full | Genus-Wide Microsatellite Primers for the Goldenrods (<i>Solidago</i>; Asteraceae) |
| title_fullStr | Genus-Wide Microsatellite Primers for the Goldenrods (<i>Solidago</i>; Asteraceae) |
| title_full_unstemmed | Genus-Wide Microsatellite Primers for the Goldenrods (<i>Solidago</i>; Asteraceae) |
| title_short | Genus-Wide Microsatellite Primers for the Goldenrods (<i>Solidago</i>; Asteraceae) |
| title_sort | genus wide microsatellite primers for the goldenrods i solidago i asteraceae |
| topic | Asteraceae Illumina sequencing polyploidy simple sequence repeat (SSR) markers Solidago |
| url | http://www.bioone.org/doi/full/10.3732/apps.1300093 |
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