Protocols to generate and isolate mouse myogenic progenitors both in vitro and in vivo

Protocols to generate and isolate mouse myogenic progenitors both in vitro and in vivo

Summary: Mouse embryonic stem cells (mESCs) can be directed to acquire cell-lineage-specific genetic programs and phenotypes by stepwise exposure to defined factors, allowing the development of in vitro models for studying disease and tissue generation. In this protocol, we describe the use of cultu...

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Bibliographic Details
Main Authors: Mamduh Khateb, Xuesong Feng, Stefania Dell’Orso, Vittorio Sartorelli
Format: Article
Language:English
Published: Elsevier 2023-03-01
Series:STAR Protocols
Subjects:
Cell Biology
Cell isolation
Flow Cytometry/Mass Cytometry
Developmental biology
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166722008814

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