Molecular characterization of Fasciola hepatica in endemic regions of Colombia
Fasciola hepatica is a zoonotic trematode that affects a wide range of hosts, including cattle, sheep, and goats. The economic impact of the parasite on the cattle industry is significant, with high losses reported worldwide. While its impact on human health was previously underestimated, recent yea...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-06-01
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| Series: | Frontiers in Veterinary Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fvets.2023.1171147/full |
| _version_ | 1797808355026141184 |
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| author | Diego Garcia-Corredor Diego Garcia-Corredor Mateo Alvarado Martín Pulido-Medellín Marina Muñoz Lissa Cruz-Saavedra Carolina Hernández Carolina Hernández Julio Cesar Giraldo Julio Cesar Giraldo Luis R. Vásquez-Arteaga Ana Cruz Morillo Coronado Juan David Ramírez Juan David Ramírez |
| author_facet | Diego Garcia-Corredor Diego Garcia-Corredor Mateo Alvarado Martín Pulido-Medellín Marina Muñoz Lissa Cruz-Saavedra Carolina Hernández Carolina Hernández Julio Cesar Giraldo Julio Cesar Giraldo Luis R. Vásquez-Arteaga Ana Cruz Morillo Coronado Juan David Ramírez Juan David Ramírez |
| author_sort | Diego Garcia-Corredor |
| collection | DOAJ |
| description | Fasciola hepatica is a zoonotic trematode that affects a wide range of hosts, including cattle, sheep, and goats. The economic impact of the parasite on the cattle industry is significant, with high losses reported worldwide. While its impact on human health was previously underestimated, recent years have seen a rise in fascioliasis cases, leading to increased interest among researchers globally. To characterize the genetic diversity and intraspecific variation of this parasite in South America, specifically in Colombia, we collected 105 adult parasites from cattle bile ducts in seven Colombian departments (Antioquia, Boyacá, Santander, Cauca, Cundinamarca, Nariño, Norte de Santander, and Santander) to assess the parasite’s phenotypic analyses, genetic diversity, and population structure. A computer image analysis system (CIAS) was applied based on standardized morphological measurements. Liver-fluke size was studied by principal component analysis (PCA). DNA sequences were obtained for nuclear markers such as the 28S, β-tubulin 3, ITS1, ITS2, and the mitochondrial marker Cytochrome Oxidase I (COI). Multiple statistical tests were performed, and the parasite’s population structure was analyzed. Maximum Likelihood (ML) phylogenetic reconstructions were carried out using the sequences obtained herein and sequences available in GenBank. Morphological results revealed that all the obtained individuals matched F. hepatica’s morphology. There was no evidence of high genetic diversity, and the absence of genetic structure at the country-level was notable, possibly caused by a demographic expansion of this trematode in Colombia or the low resolution of the molecular markers employed. Future studies are still needed to unveil the genetic population structure of F. hepatica across the country. |
| first_indexed | 2024-03-13T06:36:18Z |
| format | Article |
| id | doaj.art-b148529cd377471cbda5fbe1bbe93e81 |
| institution | Directory Open Access Journal |
| issn | 2297-1769 |
| language | English |
| last_indexed | 2024-03-13T06:36:18Z |
| publishDate | 2023-06-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Veterinary Science |
| spelling | doaj.art-b148529cd377471cbda5fbe1bbe93e812023-06-09T04:35:09ZengFrontiers Media S.A.Frontiers in Veterinary Science2297-17692023-06-011010.3389/fvets.2023.11711471171147Molecular characterization of Fasciola hepatica in endemic regions of ColombiaDiego Garcia-Corredor0Diego Garcia-Corredor1Mateo Alvarado2Martín Pulido-Medellín3Marina Muñoz4Lissa Cruz-Saavedra5Carolina Hernández6Carolina Hernández7Julio Cesar Giraldo8Julio Cesar Giraldo9Luis R. Vásquez-Arteaga10Ana Cruz Morillo Coronado11Juan David Ramírez12Juan David Ramírez13Grupo de Investigación en Medicina Veterinaria y Zootecnia (GIDIMEVETZ), Facultad de Ciencias Agropecuarias, Universidad Pedagógica y Tecnológica de Colombia (UPTC), Tunja, ColombiaCentro de Investigaciones en Microbiología y Biotecnología-UR (CIMBIUR), Facultad de Ciencias Naturales, Universidad del Rosario, Bogotá, ColombiaCentro de Investigaciones en Microbiología y Biotecnología-UR (CIMBIUR), Facultad de Ciencias Naturales, Universidad del Rosario, Bogotá, ColombiaGrupo de Investigación en Medicina Veterinaria y Zootecnia (GIDIMEVETZ), Facultad de Ciencias Agropecuarias, Universidad Pedagógica y Tecnológica de Colombia (UPTC), Tunja, ColombiaCentro de Investigaciones en Microbiología y Biotecnología-UR (CIMBIUR), Facultad de Ciencias Naturales, Universidad del Rosario, Bogotá, ColombiaCentro de Investigaciones en Microbiología y Biotecnología-UR (CIMBIUR), Facultad de Ciencias Naturales, Universidad del Rosario, Bogotá, ColombiaCentro de Investigaciones en Microbiología y Biotecnología-UR (CIMBIUR), Facultad de Ciencias Naturales, Universidad del Rosario, Bogotá, ColombiaCentro de Tecnología en Salud (CETESA), Innovaseq SAS, Bogotá, ColombiaGrupo de Investigación en Parasitología y Microbiología Tropical, Programa de Biología, Universidad INCCA de Colombia, Bogotá, ColombiaFacultad de Medicina y Ciencias de la Salud, Universidad Militar Nueva Granada, Bogotá, ColombiaCentro de Estudios en Microbiología y Parasitología, Facultad de Ciencias de la Salud, Universidad del Cauca, Popayán, ColombiaUniversidad Pedagógica y Tecnológica de Colombia, Facultad de Ciencias Agropecuarias, Tunja, ColombiaCentro de Investigaciones en Microbiología y Biotecnología-UR (CIMBIUR), Facultad de Ciencias Naturales, Universidad del Rosario, Bogotá, ColombiaMolecular Microbiology Laboratory, Department of Pathology, Molecular and Cell-Based Medicine, Icahn School of Medicine at Mount Sinai, New York, NY, United StatesFasciola hepatica is a zoonotic trematode that affects a wide range of hosts, including cattle, sheep, and goats. The economic impact of the parasite on the cattle industry is significant, with high losses reported worldwide. While its impact on human health was previously underestimated, recent years have seen a rise in fascioliasis cases, leading to increased interest among researchers globally. To characterize the genetic diversity and intraspecific variation of this parasite in South America, specifically in Colombia, we collected 105 adult parasites from cattle bile ducts in seven Colombian departments (Antioquia, Boyacá, Santander, Cauca, Cundinamarca, Nariño, Norte de Santander, and Santander) to assess the parasite’s phenotypic analyses, genetic diversity, and population structure. A computer image analysis system (CIAS) was applied based on standardized morphological measurements. Liver-fluke size was studied by principal component analysis (PCA). DNA sequences were obtained for nuclear markers such as the 28S, β-tubulin 3, ITS1, ITS2, and the mitochondrial marker Cytochrome Oxidase I (COI). Multiple statistical tests were performed, and the parasite’s population structure was analyzed. Maximum Likelihood (ML) phylogenetic reconstructions were carried out using the sequences obtained herein and sequences available in GenBank. Morphological results revealed that all the obtained individuals matched F. hepatica’s morphology. There was no evidence of high genetic diversity, and the absence of genetic structure at the country-level was notable, possibly caused by a demographic expansion of this trematode in Colombia or the low resolution of the molecular markers employed. Future studies are still needed to unveil the genetic population structure of F. hepatica across the country.https://www.frontiersin.org/articles/10.3389/fvets.2023.1171147/fullFasciola hepaticaphylogeneitc treeColombiagenetic diversitypopulation structure |
| spellingShingle | Diego Garcia-Corredor Diego Garcia-Corredor Mateo Alvarado Martín Pulido-Medellín Marina Muñoz Lissa Cruz-Saavedra Carolina Hernández Carolina Hernández Julio Cesar Giraldo Julio Cesar Giraldo Luis R. Vásquez-Arteaga Ana Cruz Morillo Coronado Juan David Ramírez Juan David Ramírez Molecular characterization of Fasciola hepatica in endemic regions of Colombia Frontiers in Veterinary Science Fasciola hepatica phylogeneitc tree Colombia genetic diversity population structure |
| title | Molecular characterization of Fasciola hepatica in endemic regions of Colombia |
| title_full | Molecular characterization of Fasciola hepatica in endemic regions of Colombia |
| title_fullStr | Molecular characterization of Fasciola hepatica in endemic regions of Colombia |
| title_full_unstemmed | Molecular characterization of Fasciola hepatica in endemic regions of Colombia |
| title_short | Molecular characterization of Fasciola hepatica in endemic regions of Colombia |
| title_sort | molecular characterization of fasciola hepatica in endemic regions of colombia |
| topic | Fasciola hepatica phylogeneitc tree Colombia genetic diversity population structure |
| url | https://www.frontiersin.org/articles/10.3389/fvets.2023.1171147/full |
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