A comparison of caspase 3 expression in the endocrine and exocrine parts of the pancreas after cladribine application according to the "leukemic" schema
The therapeutic effects of the immunosuppressive agent, cladribine, have been demonstrated by its toxicity to cells. However, its effects on healthy cells of the body is poorly understood. The aim of study was, hence, to, firstly, evaluate the morphology of the endocrine and exocrine pancreas after...
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| Format: | Article |
| Language: | English |
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Sciendo
2017-03-01
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| Series: | Current Issues in Pharmacy and Medical Sciences |
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| Online Access: | https://doi.org/10.1515/cipms-2017-0002 |
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| author | Jasinski Ludwik Chylinska-Wrzos Patrycja Lis-Sochocka Marta Wawryk-Gawda Ewelina Jodlowska-Jedrych Barbara |
| author_facet | Jasinski Ludwik Chylinska-Wrzos Patrycja Lis-Sochocka Marta Wawryk-Gawda Ewelina Jodlowska-Jedrych Barbara |
| author_sort | Jasinski Ludwik |
| collection | DOAJ |
| description | The therapeutic effects of the immunosuppressive agent, cladribine, have been demonstrated by its toxicity to cells. However, its effects on healthy cells of the body is poorly understood. The aim of study was, hence, to, firstly, evaluate the morphology of the endocrine and exocrine pancreas after the administration of cladribine according to the "leukemic" schema, and, secondly, to assess its impact on the intensity of apoptosis. The experiment was carried out on female Wistar rats which were placed within the control group KA, and the experimental groups: A and A-bis. In the experimental groups, Cladribine was administered according to the cycle used to treat human hairy cell leukemia. In group A, the material was taken 24 hours after administration of the last dose of the drug, while in group A-bis, this was done after a 4 weeks break. The reaction was assessed to be average in 80% of all cells in group A, and in 64% of all acinar cells in group KA, while in group A-bis, the majority of the exocrine cells demonstrated a lack of immunohistochemical response (72%). Moreover, most endocrine cells (60%) in group A-bis revealed a strong reaction, while in Group A, the corresponding figure is a little over 34%. A comparison of the severity of the caspase 3 expression in both the exocrine and endocrine pancreas showed significant differentiation results between the group KA and group A-bis, and between group A and A-bis (p < 0.0001). In can be concluded that endocrine cells are more sensitive to cladribine than are exocrine cells. |
| first_indexed | 2024-12-16T12:00:14Z |
| format | Article |
| id | doaj.art-b14ba021f45547cc975114bbf1fd03e5 |
| institution | Directory Open Access Journal |
| issn | 2300-6676 |
| language | English |
| last_indexed | 2024-12-16T12:00:14Z |
| publishDate | 2017-03-01 |
| publisher | Sciendo |
| record_format | Article |
| series | Current Issues in Pharmacy and Medical Sciences |
| spelling | doaj.art-b14ba021f45547cc975114bbf1fd03e52022-12-21T22:32:27ZengSciendoCurrent Issues in Pharmacy and Medical Sciences2300-66762017-03-0130191510.1515/cipms-2017-0002cipms-2017-0002A comparison of caspase 3 expression in the endocrine and exocrine parts of the pancreas after cladribine application according to the "leukemic" schemaJasinski Ludwik0Chylinska-Wrzos Patrycja1Lis-Sochocka Marta2Wawryk-Gawda Ewelina3Jodlowska-Jedrych Barbara4Chair and Department of Histology and Embryology with Experimental Cytology Unit, Medical University of Lublin, Radziwillowska 11, Lublin, PolandChair and Department of Histology and Embryology with Experimental Cytology Unit, Medical University of Lublin, Radziwillowska 11, Lublin, PolandChair and Department of Histology and Embryology with Experimental Cytology Unit, Medical University of Lublin, Radziwillowska 11, Lublin, PolandChair and Department of Histology and Embryology with Experimental Cytology Unit, Medical University of Lublin, Radziwillowska 11, Lublin, PolandJodlowska-Jedrych Chair and Department of Histology and Embryology with Experimental Cytology Unit, Medical University of Lublin, Radziwillowska 11, Lublin, PolandThe therapeutic effects of the immunosuppressive agent, cladribine, have been demonstrated by its toxicity to cells. However, its effects on healthy cells of the body is poorly understood. The aim of study was, hence, to, firstly, evaluate the morphology of the endocrine and exocrine pancreas after the administration of cladribine according to the "leukemic" schema, and, secondly, to assess its impact on the intensity of apoptosis. The experiment was carried out on female Wistar rats which were placed within the control group KA, and the experimental groups: A and A-bis. In the experimental groups, Cladribine was administered according to the cycle used to treat human hairy cell leukemia. In group A, the material was taken 24 hours after administration of the last dose of the drug, while in group A-bis, this was done after a 4 weeks break. The reaction was assessed to be average in 80% of all cells in group A, and in 64% of all acinar cells in group KA, while in group A-bis, the majority of the exocrine cells demonstrated a lack of immunohistochemical response (72%). Moreover, most endocrine cells (60%) in group A-bis revealed a strong reaction, while in Group A, the corresponding figure is a little over 34%. A comparison of the severity of the caspase 3 expression in both the exocrine and endocrine pancreas showed significant differentiation results between the group KA and group A-bis, and between group A and A-bis (p < 0.0001). In can be concluded that endocrine cells are more sensitive to cladribine than are exocrine cells.https://doi.org/10.1515/cipms-2017-0002cladribinepancreascaspase 3apoptosis |
| spellingShingle | Jasinski Ludwik Chylinska-Wrzos Patrycja Lis-Sochocka Marta Wawryk-Gawda Ewelina Jodlowska-Jedrych Barbara A comparison of caspase 3 expression in the endocrine and exocrine parts of the pancreas after cladribine application according to the "leukemic" schema Current Issues in Pharmacy and Medical Sciences cladribine pancreas caspase 3 apoptosis |
| title | A comparison of caspase 3 expression in the endocrine and exocrine parts of the pancreas after cladribine application according to the "leukemic" schema |
| title_full | A comparison of caspase 3 expression in the endocrine and exocrine parts of the pancreas after cladribine application according to the "leukemic" schema |
| title_fullStr | A comparison of caspase 3 expression in the endocrine and exocrine parts of the pancreas after cladribine application according to the "leukemic" schema |
| title_full_unstemmed | A comparison of caspase 3 expression in the endocrine and exocrine parts of the pancreas after cladribine application according to the "leukemic" schema |
| title_short | A comparison of caspase 3 expression in the endocrine and exocrine parts of the pancreas after cladribine application according to the "leukemic" schema |
| title_sort | comparison of caspase 3 expression in the endocrine and exocrine parts of the pancreas after cladribine application according to the leukemic schema |
| topic | cladribine pancreas caspase 3 apoptosis |
| url | https://doi.org/10.1515/cipms-2017-0002 |
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