Newcastle disease virus-based MERS-CoV candidate vaccine elicits high-level and lasting neutralizing antibodies in Bactrian camels
Abstract: Middle East respiratory syndrome coronavirus (MERS-CoV), a member of the Coronaviridae family, is the causative pathogen for MERS that is characterized by high fever, pneumonia, acute respiratory distress syndrome (ARDS), as well as extrapulmonary manifestations. Currently, there are no ap...
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| Format: | Article |
| Language: | English |
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Elsevier
2017-10-01
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| Series: | Journal of Integrative Agriculture |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2095311917616605 |
| _version_ | 1819108241268801536 |
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| author | Ren-qiang LIU Jin-ying GE Jin-ling WANG Yu SHAO Hui-lei ZHANG Jin-liang WANG Zhi-yuan WEN Zhi-gao BU |
| author_facet | Ren-qiang LIU Jin-ying GE Jin-ling WANG Yu SHAO Hui-lei ZHANG Jin-liang WANG Zhi-yuan WEN Zhi-gao BU |
| author_sort | Ren-qiang LIU |
| collection | DOAJ |
| description | Abstract: Middle East respiratory syndrome coronavirus (MERS-CoV), a member of the Coronaviridae family, is the causative pathogen for MERS that is characterized by high fever, pneumonia, acute respiratory distress syndrome (ARDS), as well as extrapulmonary manifestations. Currently, there are no approved treatment regimens or vaccines for MERS. Here, we generated recombinant nonvirulent Newcastle disease virus (NDV) LaSota strain expressing MERS-CoV S protein (designated as rLa-MERS-S), and evaluated its immunogenicity in mice and Bactrian camels. The results revealed that rLa-MERS-S showed similar growth properties to those of LaSota in embryonated chicken eggs, while animal immunization studies showed that rLa-MERS-S induced MERS-CoV neutralizing antibodies in mice and camels. Our findings suggest that recombinant rLa-MERS-S may be a potential MERS-CoV veterinary vaccine candidate for camels and other animals affected by MERS. |
| first_indexed | 2024-12-22T03:06:48Z |
| format | Article |
| id | doaj.art-b14bd8395f184c16a2afc557b6020048 |
| institution | Directory Open Access Journal |
| issn | 2095-3119 |
| language | English |
| last_indexed | 2024-12-22T03:06:48Z |
| publishDate | 2017-10-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Integrative Agriculture |
| spelling | doaj.art-b14bd8395f184c16a2afc557b60200482022-12-21T18:41:03ZengElsevierJournal of Integrative Agriculture2095-31192017-10-01161022642273Newcastle disease virus-based MERS-CoV candidate vaccine elicits high-level and lasting neutralizing antibodies in Bactrian camelsRen-qiang LIU0Jin-ying GE1Jin-ling WANG2Yu SHAO3Hui-lei ZHANG4Jin-liang WANG5Zhi-yuan WEN6Zhi-gao BU7State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150069, P.R.ChinaState Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150069, P.R.ChinaCollege of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot 010018, P.R.ChinaState Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150069, P.R.ChinaState Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150069, P.R.ChinaState Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150069, P.R.ChinaState Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150069, P.R.ChinaState Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150069, P.R.China; Correspondence BU Zhi-gaoAbstract: Middle East respiratory syndrome coronavirus (MERS-CoV), a member of the Coronaviridae family, is the causative pathogen for MERS that is characterized by high fever, pneumonia, acute respiratory distress syndrome (ARDS), as well as extrapulmonary manifestations. Currently, there are no approved treatment regimens or vaccines for MERS. Here, we generated recombinant nonvirulent Newcastle disease virus (NDV) LaSota strain expressing MERS-CoV S protein (designated as rLa-MERS-S), and evaluated its immunogenicity in mice and Bactrian camels. The results revealed that rLa-MERS-S showed similar growth properties to those of LaSota in embryonated chicken eggs, while animal immunization studies showed that rLa-MERS-S induced MERS-CoV neutralizing antibodies in mice and camels. Our findings suggest that recombinant rLa-MERS-S may be a potential MERS-CoV veterinary vaccine candidate for camels and other animals affected by MERS.http://www.sciencedirect.com/science/article/pii/S2095311917616605Newcastle disease virusMERS-CoVneutralizing antibodiescamels |
| spellingShingle | Ren-qiang LIU Jin-ying GE Jin-ling WANG Yu SHAO Hui-lei ZHANG Jin-liang WANG Zhi-yuan WEN Zhi-gao BU Newcastle disease virus-based MERS-CoV candidate vaccine elicits high-level and lasting neutralizing antibodies in Bactrian camels Journal of Integrative Agriculture Newcastle disease virus MERS-CoV neutralizing antibodies camels |
| title | Newcastle disease virus-based MERS-CoV candidate vaccine elicits high-level and lasting neutralizing antibodies in Bactrian camels |
| title_full | Newcastle disease virus-based MERS-CoV candidate vaccine elicits high-level and lasting neutralizing antibodies in Bactrian camels |
| title_fullStr | Newcastle disease virus-based MERS-CoV candidate vaccine elicits high-level and lasting neutralizing antibodies in Bactrian camels |
| title_full_unstemmed | Newcastle disease virus-based MERS-CoV candidate vaccine elicits high-level and lasting neutralizing antibodies in Bactrian camels |
| title_short | Newcastle disease virus-based MERS-CoV candidate vaccine elicits high-level and lasting neutralizing antibodies in Bactrian camels |
| title_sort | newcastle disease virus based mers cov candidate vaccine elicits high level and lasting neutralizing antibodies in bactrian camels |
| topic | Newcastle disease virus MERS-CoV neutralizing antibodies camels |
| url | http://www.sciencedirect.com/science/article/pii/S2095311917616605 |
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