Characterization of non-typhoid Salmonellae isolated from domestic animals and wildlife from selected areas of Zambia
Domestic animals and wildlife are considered important reservoirs from which Non-Typhoid Salmonella serotypes have emerged to cause public health concerns worldwide. Identification of the zoonotic origin of such organisms is important to understand the risk factors associated with a diversity of hos...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2020-07-01
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| Series: | Scientific African |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2468227620300831 |
| _version_ | 1819047830743941120 |
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| author | Charles M. Mubita Bwalya J. Muma King Nalubamba Girja Shanker Pandey Kenny Samui Musso Munyeme Kajihara Masahiro Yongjin Qiu Ngonda Saasa Bernard M. Hang'ombe |
| author_facet | Charles M. Mubita Bwalya J. Muma King Nalubamba Girja Shanker Pandey Kenny Samui Musso Munyeme Kajihara Masahiro Yongjin Qiu Ngonda Saasa Bernard M. Hang'ombe |
| author_sort | Charles M. Mubita |
| collection | DOAJ |
| description | Domestic animals and wildlife are considered important reservoirs from which Non-Typhoid Salmonella serotypes have emerged to cause public health concerns worldwide. Identification of the zoonotic origin of such organisms is important to understand the risk factors associated with a diversity of host types. In this study, phenotypic and genetic properties of Salmonella strains isolated from domestic animals and wildlife sources were characterized to determine their potential for causing invasive salmonellosis. The isolates were characterized into serogroups, serotypes, fermentative groups, resistotypes, and beta-lactamase activity types. Further, Salmonella plasmid virulence genes responsible for extraintestinal manifestations were determined among Salmonella serotypes from domestic animals (bovine, horse, dogs, and chickens) and wildlife (Leopard, Sable and Impala). Thirty-four Salmonella strains belonging to four sero-groups; B, C1, D1, and a non- reactive group, were isolated and identified from the various animal hosts studied. The most prevalent sero-group was D1 (50.0%; 17/34) Salmonella strains, all from domestic animals followed by a non-reactive group (29.4%; 10/34) strains. Further, 12 serotypes, were detected among the strains. The most predominant serotype was Salmonella enterica serotype Enteritidis (47.1%; 16/34) isolated from horse and chicken, followed by S. Mbandaka (8.8%; 3/34) from dog and chicken. All Salmonella isolates were resistant to at-least four antimicrobial agents. Ampicillin, erythromycin, gentamicin, nitrofurantoin and penicillin G were observed as the most frequently resisted antimicrobial agents. Salmonella Enteritidis and S. Mbandaka isolated from the horse and dog respectively, each displayed multidrug resistance to 6 antimicrobial agents. All strains harboured Salmonella invasion A gene unique to the genus, while some strains possessed Salmonella plasmid virulence locus gene. In conclusion, the present study isolated and identified multi-drug resistant Salmonella serotype strains that possess virulence genes which are of potential public health concern. |
| first_indexed | 2024-12-21T11:06:36Z |
| format | Article |
| id | doaj.art-b14dd0334e0f453d972f251ff860891b |
| institution | Directory Open Access Journal |
| issn | 2468-2276 |
| language | English |
| last_indexed | 2024-12-21T11:06:36Z |
| publishDate | 2020-07-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Scientific African |
| spelling | doaj.art-b14dd0334e0f453d972f251ff860891b2022-12-21T19:06:13ZengElsevierScientific African2468-22762020-07-018e00345Characterization of non-typhoid Salmonellae isolated from domestic animals and wildlife from selected areas of ZambiaCharles M. Mubita0Bwalya J. Muma1King Nalubamba2Girja Shanker Pandey3Kenny Samui4Musso Munyeme5Kajihara Masahiro6Yongjin Qiu7Ngonda Saasa8Bernard M. Hang'ombe9Department of Disease Control, School of Veterinary Medicine, University of Zambia. P.O. Box 32379, Lusaka, Zambia; Corresponding author.Department of Disease Control, School of Veterinary Medicine, University of Zambia. P.O. Box 32379, Lusaka, ZambiaDepartment of Disease Control, School of Veterinary Medicine, University of Zambia. P.O. Box 32379, Lusaka, ZambiaDepartment of Disease Control, School of Veterinary Medicine, University of Zambia. P.O. Box 32379, Lusaka, ZambiaDepartment of Disease Control, School of Veterinary Medicine, University of Zambia. P.O. Box 32379, Lusaka, ZambiaDepartment of Disease Control, School of Veterinary Medicine, University of Zambia. P.O. Box 32379, Lusaka, ZambiaHokkaido University Research Center for Zoonosis Control, Kita 20, Nishi 10, Kita-ku, Sapporo, JapanHokkaido University Research Center for Zoonosis Control, Kita 20, Nishi 10, Kita-ku, Sapporo, JapanDepartment of Disease Control, School of Veterinary Medicine, University of Zambia. P.O. Box 32379, Lusaka, ZambiaDepartment of Disease Control, School of Veterinary Medicine, University of Zambia. P.O. Box 32379, Lusaka, ZambiaDomestic animals and wildlife are considered important reservoirs from which Non-Typhoid Salmonella serotypes have emerged to cause public health concerns worldwide. Identification of the zoonotic origin of such organisms is important to understand the risk factors associated with a diversity of host types. In this study, phenotypic and genetic properties of Salmonella strains isolated from domestic animals and wildlife sources were characterized to determine their potential for causing invasive salmonellosis. The isolates were characterized into serogroups, serotypes, fermentative groups, resistotypes, and beta-lactamase activity types. Further, Salmonella plasmid virulence genes responsible for extraintestinal manifestations were determined among Salmonella serotypes from domestic animals (bovine, horse, dogs, and chickens) and wildlife (Leopard, Sable and Impala). Thirty-four Salmonella strains belonging to four sero-groups; B, C1, D1, and a non- reactive group, were isolated and identified from the various animal hosts studied. The most prevalent sero-group was D1 (50.0%; 17/34) Salmonella strains, all from domestic animals followed by a non-reactive group (29.4%; 10/34) strains. Further, 12 serotypes, were detected among the strains. The most predominant serotype was Salmonella enterica serotype Enteritidis (47.1%; 16/34) isolated from horse and chicken, followed by S. Mbandaka (8.8%; 3/34) from dog and chicken. All Salmonella isolates were resistant to at-least four antimicrobial agents. Ampicillin, erythromycin, gentamicin, nitrofurantoin and penicillin G were observed as the most frequently resisted antimicrobial agents. Salmonella Enteritidis and S. Mbandaka isolated from the horse and dog respectively, each displayed multidrug resistance to 6 antimicrobial agents. All strains harboured Salmonella invasion A gene unique to the genus, while some strains possessed Salmonella plasmid virulence locus gene. In conclusion, the present study isolated and identified multi-drug resistant Salmonella serotype strains that possess virulence genes which are of potential public health concern.http://www.sciencedirect.com/science/article/pii/S2468227620300831SalmonellaSerotypeResistotypeVirulence geneDomestic animals and wildlife |
| spellingShingle | Charles M. Mubita Bwalya J. Muma King Nalubamba Girja Shanker Pandey Kenny Samui Musso Munyeme Kajihara Masahiro Yongjin Qiu Ngonda Saasa Bernard M. Hang'ombe Characterization of non-typhoid Salmonellae isolated from domestic animals and wildlife from selected areas of Zambia Scientific African Salmonella Serotype Resistotype Virulence gene Domestic animals and wildlife |
| title | Characterization of non-typhoid Salmonellae isolated from domestic animals and wildlife from selected areas of Zambia |
| title_full | Characterization of non-typhoid Salmonellae isolated from domestic animals and wildlife from selected areas of Zambia |
| title_fullStr | Characterization of non-typhoid Salmonellae isolated from domestic animals and wildlife from selected areas of Zambia |
| title_full_unstemmed | Characterization of non-typhoid Salmonellae isolated from domestic animals and wildlife from selected areas of Zambia |
| title_short | Characterization of non-typhoid Salmonellae isolated from domestic animals and wildlife from selected areas of Zambia |
| title_sort | characterization of non typhoid salmonellae isolated from domestic animals and wildlife from selected areas of zambia |
| topic | Salmonella Serotype Resistotype Virulence gene Domestic animals and wildlife |
| url | http://www.sciencedirect.com/science/article/pii/S2468227620300831 |
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