Human cerebrospinal fluid promotes neuronal viability and activity of hippocampal neuronal circuits in vitro
For decades it has been hypothesized that molecules within the cerebrospinal fluid (CSF) diffuse into the brain parenchyma and influence the function of neurons. However, the functional consequences of CSF on neuronal circuits are largely unexplored and unknown. A major reason for this is the absenc...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2016-03-01
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| Series: | Frontiers in Cellular Neuroscience |
| Subjects: | |
| Online Access: | http://journal.frontiersin.org/Journal/10.3389/fncel.2016.00054/full |
| _version_ | 1828490891323506688 |
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| author | Marta ePerez-Alcazar Georgia eCulley Tim eLyckenvik Kristoffer eMobarrez Andreas eBjorefeldt Pontus eWasling Henrik eSeth Fredrik eAsztely Andrea eHarrer Bernhard eIglseder Ludwig eAigner Eric eHanse Sebastian eIlles Sebastian eIlles |
| author_facet | Marta ePerez-Alcazar Georgia eCulley Tim eLyckenvik Kristoffer eMobarrez Andreas eBjorefeldt Pontus eWasling Henrik eSeth Fredrik eAsztely Andrea eHarrer Bernhard eIglseder Ludwig eAigner Eric eHanse Sebastian eIlles Sebastian eIlles |
| author_sort | Marta ePerez-Alcazar |
| collection | DOAJ |
| description | For decades it has been hypothesized that molecules within the cerebrospinal fluid (CSF) diffuse into the brain parenchyma and influence the function of neurons. However, the functional consequences of CSF on neuronal circuits are largely unexplored and unknown. A major reason for this is the absence of appropriate neuronal in vitro model systems, and it is uncertain if neurons cultured in pure CSF survive and preserve electrophysiological functionality in vitro. In this article we present an approach to address how human CSF (hCSF) influences neuronal circuits in vitro. We validate our approach by comparing the morphology, viability, and electrophysiological function of single neurons and at the network level in rat organotypic slice and primary neuronal cultures cultivated either in hCSF or in defined standard culture media. Our results demonstrate that rodent hippocampal slices and primary neurons cultured in hCSF maintain neuronal morphology and preserve synaptic transmission. Importantly, we show that hCSF increases neuronal viability and the number of electrophysiologically active neurons in comparison to the culture media. In summary, our data indicate that hCSF represents a physiological environment for neurons in vitro and a superior culture condition compared to the defined standard media. Moreover, this experimental approach paves the way to assess the functional consequences of CSF on neuronal circuits as well as suggesting a novel strategy for CNS disease modeling. |
| first_indexed | 2024-12-11T10:51:53Z |
| format | Article |
| id | doaj.art-b151ab695b8f4879bc480fc0b043cfa5 |
| institution | Directory Open Access Journal |
| issn | 1662-5102 |
| language | English |
| last_indexed | 2024-12-11T10:51:53Z |
| publishDate | 2016-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cellular Neuroscience |
| spelling | doaj.art-b151ab695b8f4879bc480fc0b043cfa52022-12-22T01:10:15ZengFrontiers Media S.A.Frontiers in Cellular Neuroscience1662-51022016-03-011010.3389/fncel.2016.00054184928Human cerebrospinal fluid promotes neuronal viability and activity of hippocampal neuronal circuits in vitroMarta ePerez-Alcazar0Georgia eCulley1Tim eLyckenvik2Kristoffer eMobarrez3Andreas eBjorefeldt4Pontus eWasling5Henrik eSeth6Fredrik eAsztely7Andrea eHarrer8Bernhard eIglseder9Ludwig eAigner10Eric eHanse11Sebastian eIlles12Sebastian eIlles13University of Gothenburg, The Sahlgrenska AcademyUniversity of Gothenburg, The Sahlgrenska AcademyUniversity of Gothenburg, The Sahlgrenska AcademyUniversity of Gothenburg, The Sahlgrenska AcademyUniversity of Gothenburg, The Sahlgrenska AcademyUniversity of Gothenburg, The Sahlgrenska AcademyUniversity of Gothenburg, The Sahlgrenska AcademyUniversity of Gothenburg, The Sahlgrenska AcademyChristian-Doppler-Klinik, Paracelsus Medical UniversityChristian-Doppler-Klinik, Paracelsus Medical UniversityParacelsus Medical UniversityUniversity of Gothenburg, The Sahlgrenska AcademyParacelsus Medical UniversityUniversity of Gothenburg, The Sahlgrenska AcademyFor decades it has been hypothesized that molecules within the cerebrospinal fluid (CSF) diffuse into the brain parenchyma and influence the function of neurons. However, the functional consequences of CSF on neuronal circuits are largely unexplored and unknown. A major reason for this is the absence of appropriate neuronal in vitro model systems, and it is uncertain if neurons cultured in pure CSF survive and preserve electrophysiological functionality in vitro. In this article we present an approach to address how human CSF (hCSF) influences neuronal circuits in vitro. We validate our approach by comparing the morphology, viability, and electrophysiological function of single neurons and at the network level in rat organotypic slice and primary neuronal cultures cultivated either in hCSF or in defined standard culture media. Our results demonstrate that rodent hippocampal slices and primary neurons cultured in hCSF maintain neuronal morphology and preserve synaptic transmission. Importantly, we show that hCSF increases neuronal viability and the number of electrophysiologically active neurons in comparison to the culture media. In summary, our data indicate that hCSF represents a physiological environment for neurons in vitro and a superior culture condition compared to the defined standard media. Moreover, this experimental approach paves the way to assess the functional consequences of CSF on neuronal circuits as well as suggesting a novel strategy for CNS disease modeling.http://journal.frontiersin.org/Journal/10.3389/fncel.2016.00054/fullHuman cerebrospinal fluidOrganotypic hippocampal slice cultureshippocampal neuronal functionhippocampal neuronal survivalhippocampal neuronal culturesmulti-electrode array technology |
| spellingShingle | Marta ePerez-Alcazar Georgia eCulley Tim eLyckenvik Kristoffer eMobarrez Andreas eBjorefeldt Pontus eWasling Henrik eSeth Fredrik eAsztely Andrea eHarrer Bernhard eIglseder Ludwig eAigner Eric eHanse Sebastian eIlles Sebastian eIlles Human cerebrospinal fluid promotes neuronal viability and activity of hippocampal neuronal circuits in vitro Frontiers in Cellular Neuroscience Human cerebrospinal fluid Organotypic hippocampal slice cultures hippocampal neuronal function hippocampal neuronal survival hippocampal neuronal cultures multi-electrode array technology |
| title | Human cerebrospinal fluid promotes neuronal viability and activity of hippocampal neuronal circuits in vitro |
| title_full | Human cerebrospinal fluid promotes neuronal viability and activity of hippocampal neuronal circuits in vitro |
| title_fullStr | Human cerebrospinal fluid promotes neuronal viability and activity of hippocampal neuronal circuits in vitro |
| title_full_unstemmed | Human cerebrospinal fluid promotes neuronal viability and activity of hippocampal neuronal circuits in vitro |
| title_short | Human cerebrospinal fluid promotes neuronal viability and activity of hippocampal neuronal circuits in vitro |
| title_sort | human cerebrospinal fluid promotes neuronal viability and activity of hippocampal neuronal circuits in vitro |
| topic | Human cerebrospinal fluid Organotypic hippocampal slice cultures hippocampal neuronal function hippocampal neuronal survival hippocampal neuronal cultures multi-electrode array technology |
| url | http://journal.frontiersin.org/Journal/10.3389/fncel.2016.00054/full |
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