β-(1,3)-D-glucan from <em>Pleurotus ostreatus</em> correlates with lower plasma IL-6, IL-1β, HOMA-IR, and higher pancreatic beta cell count in High-Fat and High-Fructose Diet (HFFD) rats
Introduction: The increasing consumption of high-fat and high-fructose foods contributes to the increasing prevalence of global obesity. Low-grade chronic inflammation in obesity is a significant risk factor for insulin resistance and type 2 diabetes. Therefore, this study aimed to determine the ef...
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| Format: | Article |
| Language: | English |
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PAGEPress Publications
2023-02-01
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| Series: | Healthcare in Low-resource Settings |
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| Online Access: | https://www.pagepressjournals.org/index.php/hls/article/view/11165 |
| _version_ | 1797224693132951552 |
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| author | Alma Maghfirotun Innayah Elvira Nur Sa’idah Hariani Husnul Khotimah Inggita Kusumastuty Ema Pristi Yunita Dian Handayani |
| author_facet | Alma Maghfirotun Innayah Elvira Nur Sa’idah Hariani Husnul Khotimah Inggita Kusumastuty Ema Pristi Yunita Dian Handayani |
| author_sort | Alma Maghfirotun Innayah |
| collection | DOAJ |
| description |
Introduction: The increasing consumption of high-fat and high-fructose foods contributes to the increasing prevalence of global obesity. Low-grade chronic inflammation in obesity is a significant risk factor for insulin resistance and type 2 diabetes. Therefore, this study aimed to determine the effect of β-(1,3)-D-glucan from oyster mushroom (Pleurotus ostreatus) extract on rats fed with a high-fat and high-fructose diet.
Design and Methods: This experimental study was conducted on 35 male Sprague-Dawley rats aged eight weeks. The rats were divided into groups given a normal (N) diet, a high-fat and high-fructose diet (HFFD), D1 (HFFD+125 mg/kg BW β-glucan), D2 (HFFD+250 mg/kg BW β glucan), and D3 (HFFD+375 mg/kg BW β-glucan) with an intervention of 14 weeks. IL-6 and IL-1β levels were measured by the ELISA method, while HOMA-IR (Homeostatic Model Assessment for Insulin Resistance) was calculated by the fasting insulin (ng/mL) x fasting blood glucose (mg/dL)/405 formula. Pancreatic beta-cell counts were measured by hematoxylin and eosin (H&E) staining.
Results: The results showed no differences in IL-6 and IL-1β between the treatment groups. However, there were significant differences in HOMA-IR and pancreatic beta-cell counts between groups. There were negative correlations between the dose of β-glucan and IL-6, IL-1β, and HOMA-IR levels. Also, there was a positive correlation between the dose of β-glucan and the number of pancreatic beta cells.
Conclusions: Administration of β-(1,3)-D-glucan from oyster mushroom (Pleurotus ostreatus) extract prevented hyperglycemia and insulin resistance, also reduced inflammation in rats fed with HFFD regardless of weight gain.
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| first_indexed | 2024-04-24T13:57:10Z |
| format | Article |
| id | doaj.art-b152a85974c74d3d98f630d878395bfc |
| institution | Directory Open Access Journal |
| issn | 2281-7824 |
| language | English |
| last_indexed | 2024-04-24T13:57:10Z |
| publishDate | 2023-02-01 |
| publisher | PAGEPress Publications |
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| series | Healthcare in Low-resource Settings |
| spelling | doaj.art-b152a85974c74d3d98f630d878395bfc2024-04-03T17:28:46ZengPAGEPress PublicationsHealthcare in Low-resource Settings2281-78242023-02-0111s110.4081/hls.2023.11165β-(1,3)-D-glucan from <em>Pleurotus ostreatus</em> correlates with lower plasma IL-6, IL-1β, HOMA-IR, and higher pancreatic beta cell count in High-Fat and High-Fructose Diet (HFFD) rats Alma Maghfirotun Innayah0Elvira Nur Sa’idah Hariani1Husnul Khotimah2Inggita Kusumastuty3Ema Pristi Yunita4Dian Handayani5Master Program in Biomedical Sciences, Faculty of Medicine, Universitas Brawijaya, MalangDepartment of Nutrition Science, Faculty of Health Sciences, Universitas Brawijaya, MalangMaster Program in Biomedical Sciences; Pharmacology Department, Faculty of Medicine, Universitas Brawijaya, MalangDepartment of Nutrition Science, Faculty of Health Sciences, Universitas Brawijaya, MalangDepartment of Pharmacy, Faculty of Medicine; Research Center of Smart Molecule of Natural Genetic Resources (SMONAGENES), Universitas Brawijaya, MalangDepartment of Nutrition Science, Faculty of Health Sciences; Research Center of Smart Molecule of Natural Genetic Resources (SMONAGENES), Universitas Brawijaya, Malang Introduction: The increasing consumption of high-fat and high-fructose foods contributes to the increasing prevalence of global obesity. Low-grade chronic inflammation in obesity is a significant risk factor for insulin resistance and type 2 diabetes. Therefore, this study aimed to determine the effect of β-(1,3)-D-glucan from oyster mushroom (Pleurotus ostreatus) extract on rats fed with a high-fat and high-fructose diet. Design and Methods: This experimental study was conducted on 35 male Sprague-Dawley rats aged eight weeks. The rats were divided into groups given a normal (N) diet, a high-fat and high-fructose diet (HFFD), D1 (HFFD+125 mg/kg BW β-glucan), D2 (HFFD+250 mg/kg BW β glucan), and D3 (HFFD+375 mg/kg BW β-glucan) with an intervention of 14 weeks. IL-6 and IL-1β levels were measured by the ELISA method, while HOMA-IR (Homeostatic Model Assessment for Insulin Resistance) was calculated by the fasting insulin (ng/mL) x fasting blood glucose (mg/dL)/405 formula. Pancreatic beta-cell counts were measured by hematoxylin and eosin (H&E) staining. Results: The results showed no differences in IL-6 and IL-1β between the treatment groups. However, there were significant differences in HOMA-IR and pancreatic beta-cell counts between groups. There were negative correlations between the dose of β-glucan and IL-6, IL-1β, and HOMA-IR levels. Also, there was a positive correlation between the dose of β-glucan and the number of pancreatic beta cells. Conclusions: Administration of β-(1,3)-D-glucan from oyster mushroom (Pleurotus ostreatus) extract prevented hyperglycemia and insulin resistance, also reduced inflammation in rats fed with HFFD regardless of weight gain. https://www.pagepressjournals.org/index.php/hls/article/view/11165β-(1,3)-D-glucanHOMA-IRIL-6IL-1βpancreatic beta cell |
| spellingShingle | Alma Maghfirotun Innayah Elvira Nur Sa’idah Hariani Husnul Khotimah Inggita Kusumastuty Ema Pristi Yunita Dian Handayani β-(1,3)-D-glucan from <em>Pleurotus ostreatus</em> correlates with lower plasma IL-6, IL-1β, HOMA-IR, and higher pancreatic beta cell count in High-Fat and High-Fructose Diet (HFFD) rats Healthcare in Low-resource Settings β-(1,3)-D-glucan HOMA-IR IL-6 IL-1β pancreatic beta cell |
| title | β-(1,3)-D-glucan from <em>Pleurotus ostreatus</em> correlates with lower plasma IL-6, IL-1β, HOMA-IR, and higher pancreatic beta cell count in High-Fat and High-Fructose Diet (HFFD) rats |
| title_full | β-(1,3)-D-glucan from <em>Pleurotus ostreatus</em> correlates with lower plasma IL-6, IL-1β, HOMA-IR, and higher pancreatic beta cell count in High-Fat and High-Fructose Diet (HFFD) rats |
| title_fullStr | β-(1,3)-D-glucan from <em>Pleurotus ostreatus</em> correlates with lower plasma IL-6, IL-1β, HOMA-IR, and higher pancreatic beta cell count in High-Fat and High-Fructose Diet (HFFD) rats |
| title_full_unstemmed | β-(1,3)-D-glucan from <em>Pleurotus ostreatus</em> correlates with lower plasma IL-6, IL-1β, HOMA-IR, and higher pancreatic beta cell count in High-Fat and High-Fructose Diet (HFFD) rats |
| title_short | β-(1,3)-D-glucan from <em>Pleurotus ostreatus</em> correlates with lower plasma IL-6, IL-1β, HOMA-IR, and higher pancreatic beta cell count in High-Fat and High-Fructose Diet (HFFD) rats |
| title_sort | β 1 3 d glucan from em pleurotus ostreatus em correlates with lower plasma il 6 il 1β homa ir and higher pancreatic beta cell count in high fat and high fructose diet hffd rats |
| topic | β-(1,3)-D-glucan HOMA-IR IL-6 IL-1β pancreatic beta cell |
| url | https://www.pagepressjournals.org/index.php/hls/article/view/11165 |
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