Acclimation of liverwort Marchantia polymorpha to physiological drought reveals important roles of antioxidant enzymes, proline and abscisic acid in land plant adaptation to osmotic stress
Liverwort Marchantia polymorpha is considered as the key species for addressing a myriad of questions in plant biology. Exploration of drought tolerance mechanism(s) in this group of land plants offers a platform to identify the early adaptive mechanisms involved in drought tolerance. The current st...
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PeerJ Inc.
2021-11-01
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| Online Access: | https://peerj.com/articles/12419.pdf |
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| author | Totan Kumar Ghosh Naznin Haque Tompa Md. Mezanur Rahman Mohammed Mohi-Ud-Din S. M. Zubair Al-Meraj Md. Sanaullah Biswas Mohammad Golam Mostofa |
| author_facet | Totan Kumar Ghosh Naznin Haque Tompa Md. Mezanur Rahman Mohammed Mohi-Ud-Din S. M. Zubair Al-Meraj Md. Sanaullah Biswas Mohammad Golam Mostofa |
| author_sort | Totan Kumar Ghosh |
| collection | DOAJ |
| description | Liverwort Marchantia polymorpha is considered as the key species for addressing a myriad of questions in plant biology. Exploration of drought tolerance mechanism(s) in this group of land plants offers a platform to identify the early adaptive mechanisms involved in drought tolerance. The current study aimed at elucidating the drought acclimation mechanisms in liverwort’s model M. polymorpha. The gemmae, asexual reproductive units of M. polymorpha, were exposed to sucrose (0.2 M), mannitol (0.5 M) and polyethylene glycol (PEG, 10%) for inducing physiological drought to investigate their effects at morphological, physiological and biochemical levels. Our results showed that drought exposure led to extreme growth inhibition, disruption of membrane stability and reduction in photosynthetic pigment contents in M. polymorpha. The increased accumulation of hydrogen peroxide and malondialdehyde, and the rate of electrolyte leakage in the gemmalings of M. polymorpha indicated an evidence of drought-caused oxidative stress. The gemmalings showed significant induction of the activities of key antioxidant enzymes, including superoxide dismutase, catalase, ascorbate peroxidase, dehydroascorbate reductase and glutathione S-transferase, and total antioxidant activity in response to increased oxidative stress under drought. Importantly, to counteract the drought effects, the gemmalings also accumulated a significant amount of proline, which coincided with the evolutionary presence of proline biosynthesis gene Δ1-pyrroline-5-carboxylate synthase 1 (P5CS1) in land plants. Furthermore, the application of exogenous abscisic acid (ABA) reduced drought-induced tissue damage and improved the activities of antioxidant enzymes and accumulation of proline, implying an archetypal role of this phytohormone in M. polymorpha for drought tolerance. We conclude that physiological drought tolerance mechanisms governed by the cellular antioxidants, proline and ABA were adopted in liverwort M. polymorpha, and that these findings have important implications in aiding our understanding of osmotic stress acclimation processes in land plants. |
| first_indexed | 2024-03-09T06:23:04Z |
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| language | English |
| last_indexed | 2024-03-09T06:23:04Z |
| publishDate | 2021-11-01 |
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| spelling | doaj.art-b152e2aa70fd487fb810c96c76b804332023-12-03T11:33:22ZengPeerJ Inc.PeerJ2167-83592021-11-019e1241910.7717/peerj.12419Acclimation of liverwort Marchantia polymorpha to physiological drought reveals important roles of antioxidant enzymes, proline and abscisic acid in land plant adaptation to osmotic stressTotan Kumar Ghosh0Naznin Haque Tompa1Md. Mezanur Rahman2Mohammed Mohi-Ud-Din3S. M. Zubair Al-Meraj4Md. Sanaullah Biswas5Mohammad Golam Mostofa6Department of Crop Botany, Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur, BangladeshDepartment of Crop Botany, Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur, BangladeshInstitute of Genomics for Crop Abiotic Stress Tolerance, Department of Plant and Soil Science, Texas Tech University, Lubbock, Texas, United StatesDepartment of Crop Botany, Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur, BangladeshDepartment of Crop Botany, Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur, BangladeshDepartment of Horticulture, Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur, BangladeshInstitute of Genomics for Crop Abiotic Stress Tolerance, Department of Plant and Soil Science, Texas Tech University, Lubbock, Texas, United StatesLiverwort Marchantia polymorpha is considered as the key species for addressing a myriad of questions in plant biology. Exploration of drought tolerance mechanism(s) in this group of land plants offers a platform to identify the early adaptive mechanisms involved in drought tolerance. The current study aimed at elucidating the drought acclimation mechanisms in liverwort’s model M. polymorpha. The gemmae, asexual reproductive units of M. polymorpha, were exposed to sucrose (0.2 M), mannitol (0.5 M) and polyethylene glycol (PEG, 10%) for inducing physiological drought to investigate their effects at morphological, physiological and biochemical levels. Our results showed that drought exposure led to extreme growth inhibition, disruption of membrane stability and reduction in photosynthetic pigment contents in M. polymorpha. The increased accumulation of hydrogen peroxide and malondialdehyde, and the rate of electrolyte leakage in the gemmalings of M. polymorpha indicated an evidence of drought-caused oxidative stress. The gemmalings showed significant induction of the activities of key antioxidant enzymes, including superoxide dismutase, catalase, ascorbate peroxidase, dehydroascorbate reductase and glutathione S-transferase, and total antioxidant activity in response to increased oxidative stress under drought. Importantly, to counteract the drought effects, the gemmalings also accumulated a significant amount of proline, which coincided with the evolutionary presence of proline biosynthesis gene Δ1-pyrroline-5-carboxylate synthase 1 (P5CS1) in land plants. Furthermore, the application of exogenous abscisic acid (ABA) reduced drought-induced tissue damage and improved the activities of antioxidant enzymes and accumulation of proline, implying an archetypal role of this phytohormone in M. polymorpha for drought tolerance. We conclude that physiological drought tolerance mechanisms governed by the cellular antioxidants, proline and ABA were adopted in liverwort M. polymorpha, and that these findings have important implications in aiding our understanding of osmotic stress acclimation processes in land plants.https://peerj.com/articles/12419.pdfAbscisic acidAntioxidant systemDrought acclimationM. polymorphaMembrane damageOxidative stress |
| spellingShingle | Totan Kumar Ghosh Naznin Haque Tompa Md. Mezanur Rahman Mohammed Mohi-Ud-Din S. M. Zubair Al-Meraj Md. Sanaullah Biswas Mohammad Golam Mostofa Acclimation of liverwort Marchantia polymorpha to physiological drought reveals important roles of antioxidant enzymes, proline and abscisic acid in land plant adaptation to osmotic stress PeerJ Abscisic acid Antioxidant system Drought acclimation M. polymorpha Membrane damage Oxidative stress |
| title | Acclimation of liverwort Marchantia polymorpha to physiological drought reveals important roles of antioxidant enzymes, proline and abscisic acid in land plant adaptation to osmotic stress |
| title_full | Acclimation of liverwort Marchantia polymorpha to physiological drought reveals important roles of antioxidant enzymes, proline and abscisic acid in land plant adaptation to osmotic stress |
| title_fullStr | Acclimation of liverwort Marchantia polymorpha to physiological drought reveals important roles of antioxidant enzymes, proline and abscisic acid in land plant adaptation to osmotic stress |
| title_full_unstemmed | Acclimation of liverwort Marchantia polymorpha to physiological drought reveals important roles of antioxidant enzymes, proline and abscisic acid in land plant adaptation to osmotic stress |
| title_short | Acclimation of liverwort Marchantia polymorpha to physiological drought reveals important roles of antioxidant enzymes, proline and abscisic acid in land plant adaptation to osmotic stress |
| title_sort | acclimation of liverwort marchantia polymorpha to physiological drought reveals important roles of antioxidant enzymes proline and abscisic acid in land plant adaptation to osmotic stress |
| topic | Abscisic acid Antioxidant system Drought acclimation M. polymorpha Membrane damage Oxidative stress |
| url | https://peerj.com/articles/12419.pdf |
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