Mid-cell migration of the chromosomal terminus is coupled to origin segregation in Escherichia coli
Abstract Bacterial chromosomes are dynamically and spatially organised within cells. In slow-growing Escherichia coli, the chromosomal terminus is initially located at the new pole and must therefore migrate to midcell during replication to reproduce the same pattern in the daughter cells. Here, we...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2023-11-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-023-43351-7 |
| _version_ | 1797558330168705024 |
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| author | Ismath Sadhir Seán M. Murray |
| author_facet | Ismath Sadhir Seán M. Murray |
| author_sort | Ismath Sadhir |
| collection | DOAJ |
| description | Abstract Bacterial chromosomes are dynamically and spatially organised within cells. In slow-growing Escherichia coli, the chromosomal terminus is initially located at the new pole and must therefore migrate to midcell during replication to reproduce the same pattern in the daughter cells. Here, we use high-throughput time-lapse microscopy to quantify this transition, its timing and its relationship to chromosome segregation. We find that terminus centralisation is a rapid discrete event that occurs ~25 min after initial separation of duplicated origins and ~50 min before the onset of bulk nucleoid segregation but with substantial variation between cells. Despite this variation, its movement is tightly coincident with the completion of origin segregation, even in the absence of its linkage to the divisome, suggesting a coupling between these two events. Indeed, we find that terminus centralisation does not occur if origin segregation away from mid-cell is disrupted, which results in daughter cells having an inverted chromosome organisation. Overall, our study quantifies the choreography of origin-terminus positioning and identifies an unexplored connection between these loci, furthering our understanding of chromosome segregation in this bacterium. |
| first_indexed | 2024-03-10T17:28:44Z |
| format | Article |
| id | doaj.art-b1530731fa5f4427a1f938c23500f08b |
| institution | Directory Open Access Journal |
| issn | 2041-1723 |
| language | English |
| last_indexed | 2024-03-10T17:28:44Z |
| publishDate | 2023-11-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj.art-b1530731fa5f4427a1f938c23500f08b2023-11-20T10:05:57ZengNature PortfolioNature Communications2041-17232023-11-0114111210.1038/s41467-023-43351-7Mid-cell migration of the chromosomal terminus is coupled to origin segregation in Escherichia coliIsmath Sadhir0Seán M. Murray1Max Planck Institute for Terrestrial Microbiology and LOEWE Centre for Synthetic Microbiology (SYNMIKRO)Max Planck Institute for Terrestrial Microbiology and LOEWE Centre for Synthetic Microbiology (SYNMIKRO)Abstract Bacterial chromosomes are dynamically and spatially organised within cells. In slow-growing Escherichia coli, the chromosomal terminus is initially located at the new pole and must therefore migrate to midcell during replication to reproduce the same pattern in the daughter cells. Here, we use high-throughput time-lapse microscopy to quantify this transition, its timing and its relationship to chromosome segregation. We find that terminus centralisation is a rapid discrete event that occurs ~25 min after initial separation of duplicated origins and ~50 min before the onset of bulk nucleoid segregation but with substantial variation between cells. Despite this variation, its movement is tightly coincident with the completion of origin segregation, even in the absence of its linkage to the divisome, suggesting a coupling between these two events. Indeed, we find that terminus centralisation does not occur if origin segregation away from mid-cell is disrupted, which results in daughter cells having an inverted chromosome organisation. Overall, our study quantifies the choreography of origin-terminus positioning and identifies an unexplored connection between these loci, furthering our understanding of chromosome segregation in this bacterium.https://doi.org/10.1038/s41467-023-43351-7 |
| spellingShingle | Ismath Sadhir Seán M. Murray Mid-cell migration of the chromosomal terminus is coupled to origin segregation in Escherichia coli Nature Communications |
| title | Mid-cell migration of the chromosomal terminus is coupled to origin segregation in Escherichia coli |
| title_full | Mid-cell migration of the chromosomal terminus is coupled to origin segregation in Escherichia coli |
| title_fullStr | Mid-cell migration of the chromosomal terminus is coupled to origin segregation in Escherichia coli |
| title_full_unstemmed | Mid-cell migration of the chromosomal terminus is coupled to origin segregation in Escherichia coli |
| title_short | Mid-cell migration of the chromosomal terminus is coupled to origin segregation in Escherichia coli |
| title_sort | mid cell migration of the chromosomal terminus is coupled to origin segregation in escherichia coli |
| url | https://doi.org/10.1038/s41467-023-43351-7 |
| work_keys_str_mv | AT ismathsadhir midcellmigrationofthechromosomalterminusiscoupledtooriginsegregationinescherichiacoli AT seanmmurray midcellmigrationofthechromosomalterminusiscoupledtooriginsegregationinescherichiacoli |