Expression Analysis of Molecular Chaperones Hsp70 and Hsp90 on Development and Metabolism of Different Organs and Testis in Cattle (Cattle–yak and Yak)
Hsp70 and Hsp90 play an important role in testis development and spermatogenesis regulation, but the exact connection between Hsp70 and Hsp90 and metabolic stress in cattle is unclear. Here, we focused on the male cattle–yak and yak, investigated the expression and localization of Hsp70 and Hsp90 in...
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MDPI AG
2022-11-01
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| Series: | Metabolites |
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| Online Access: | https://www.mdpi.com/2218-1989/12/11/1114 |
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| author | Yan Cui Penggang Liu Sijiu Yu Junfeng He Seth Y. Afedo Shengnan Zou Qian Zhang Jun Liu Liangli Song Yuanfang Xu Ting Wang Hui Li |
| author_facet | Yan Cui Penggang Liu Sijiu Yu Junfeng He Seth Y. Afedo Shengnan Zou Qian Zhang Jun Liu Liangli Song Yuanfang Xu Ting Wang Hui Li |
| author_sort | Yan Cui |
| collection | DOAJ |
| description | Hsp70 and Hsp90 play an important role in testis development and spermatogenesis regulation, but the exact connection between Hsp70 and Hsp90 and metabolic stress in cattle is unclear. Here, we focused on the male cattle–yak and yak, investigated the expression and localization of Hsp70 and Hsp90 in their tissues, and explored the influence of these factors on development and metabolism. In our study, a total of 54 cattle (24 cattle–yaks and 30 yaks; aged 1 day to 10 years) were examined. The Hsp90 mRNA of the cattle–yak was first cloned and compared with that of the yak, and variation in the amino acid sequence was found, which led to differences in protein spatial structure. Using real-time quantitative PCR (RT-qPCR) and Western blot (WB) techniques, we investigated whether the expression of Hsp70 and Hsp90 mRNA and protein are different in the cattle–yak and yak. We found a disparity in Hsp70 and Hsp90 mRNA and protein expression in different non-reproductive organs and in testicular tissues at different stages of development, while high expression was observed in the testes of both juveniles and adults. Moreover, it was intriguing to observe that Hsp70 expression was significantly high in the yak, whereas Hsp90 was high in the cattle–yak (<i>p</i> < 0.01). We also examined the location of Hsp70 and Hsp90 in the testis by immunohistochemical (IHC) and immunofluorescence (IF) techniques, and the results showed that Hsp70 and Hsp90 were positive in the epithelial cells, spermatogenic cells, and mesenchymal cells. In summary, our study proved that Hsp70 and Hsp90 expressions were different in different tissues (kidney, heart, cerebellum, liver, lung, spleen, and testis), and Hsp90 expression was high in the testis of the cattle–yak, suggesting that dysplasia of the cattle–yak may correlate with an over-metabolism of Hsp90. |
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| language | English |
| last_indexed | 2024-03-09T18:09:11Z |
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| series | Metabolites |
| spelling | doaj.art-b15507149ebd44b3bcbb57a353453e2f2023-11-24T09:13:15ZengMDPI AGMetabolites2218-19892022-11-011211111410.3390/metabo12111114Expression Analysis of Molecular Chaperones Hsp70 and Hsp90 on Development and Metabolism of Different Organs and Testis in Cattle (Cattle–yak and Yak)Yan Cui0Penggang Liu1Sijiu Yu2Junfeng He3Seth Y. Afedo4Shengnan Zou5Qian Zhang6Jun Liu7Liangli Song8Yuanfang Xu9Ting Wang10Hui Li11College of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730070, ChinaCollege of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730070, ChinaCollege of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730070, ChinaCollege of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730070, ChinaCollege of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730070, ChinaCollege of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730070, ChinaCollege of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730070, ChinaCollege of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730070, ChinaCollege of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730070, ChinaCollege of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730070, ChinaCollege of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730070, ChinaCollege of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730070, ChinaHsp70 and Hsp90 play an important role in testis development and spermatogenesis regulation, but the exact connection between Hsp70 and Hsp90 and metabolic stress in cattle is unclear. Here, we focused on the male cattle–yak and yak, investigated the expression and localization of Hsp70 and Hsp90 in their tissues, and explored the influence of these factors on development and metabolism. In our study, a total of 54 cattle (24 cattle–yaks and 30 yaks; aged 1 day to 10 years) were examined. The Hsp90 mRNA of the cattle–yak was first cloned and compared with that of the yak, and variation in the amino acid sequence was found, which led to differences in protein spatial structure. Using real-time quantitative PCR (RT-qPCR) and Western blot (WB) techniques, we investigated whether the expression of Hsp70 and Hsp90 mRNA and protein are different in the cattle–yak and yak. We found a disparity in Hsp70 and Hsp90 mRNA and protein expression in different non-reproductive organs and in testicular tissues at different stages of development, while high expression was observed in the testes of both juveniles and adults. Moreover, it was intriguing to observe that Hsp70 expression was significantly high in the yak, whereas Hsp90 was high in the cattle–yak (<i>p</i> < 0.01). We also examined the location of Hsp70 and Hsp90 in the testis by immunohistochemical (IHC) and immunofluorescence (IF) techniques, and the results showed that Hsp70 and Hsp90 were positive in the epithelial cells, spermatogenic cells, and mesenchymal cells. In summary, our study proved that Hsp70 and Hsp90 expressions were different in different tissues (kidney, heart, cerebellum, liver, lung, spleen, and testis), and Hsp90 expression was high in the testis of the cattle–yak, suggesting that dysplasia of the cattle–yak may correlate with an over-metabolism of Hsp90.https://www.mdpi.com/2218-1989/12/11/1114Hsp70 and Hsp90expressiondifferent organsmetabolismcattle |
| spellingShingle | Yan Cui Penggang Liu Sijiu Yu Junfeng He Seth Y. Afedo Shengnan Zou Qian Zhang Jun Liu Liangli Song Yuanfang Xu Ting Wang Hui Li Expression Analysis of Molecular Chaperones Hsp70 and Hsp90 on Development and Metabolism of Different Organs and Testis in Cattle (Cattle–yak and Yak) Metabolites Hsp70 and Hsp90 expression different organs metabolism cattle |
| title | Expression Analysis of Molecular Chaperones Hsp70 and Hsp90 on Development and Metabolism of Different Organs and Testis in Cattle (Cattle–yak and Yak) |
| title_full | Expression Analysis of Molecular Chaperones Hsp70 and Hsp90 on Development and Metabolism of Different Organs and Testis in Cattle (Cattle–yak and Yak) |
| title_fullStr | Expression Analysis of Molecular Chaperones Hsp70 and Hsp90 on Development and Metabolism of Different Organs and Testis in Cattle (Cattle–yak and Yak) |
| title_full_unstemmed | Expression Analysis of Molecular Chaperones Hsp70 and Hsp90 on Development and Metabolism of Different Organs and Testis in Cattle (Cattle–yak and Yak) |
| title_short | Expression Analysis of Molecular Chaperones Hsp70 and Hsp90 on Development and Metabolism of Different Organs and Testis in Cattle (Cattle–yak and Yak) |
| title_sort | expression analysis of molecular chaperones hsp70 and hsp90 on development and metabolism of different organs and testis in cattle cattle yak and yak |
| topic | Hsp70 and Hsp90 expression different organs metabolism cattle |
| url | https://www.mdpi.com/2218-1989/12/11/1114 |
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