Gliotoxin elicits immunotoxicity in the early innate immune system of ducks
ABSTRACT: Gliotoxin (GT) belongs to the epipolythiodioxopiperazine (ETP) family, which is considered a crucial virulence determinant among the secondary metabolites produced by Aspergillus fumigatus. The metabolites are commonly found in food and feed, contributing to the invasion and immune escape...
| Main Authors: | , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2024-06-01
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| Series: | Poultry Science |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0032579124002980 |
| _version_ | 1797198806190653440 |
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| author | Zhengkai Wei Qinqin Jin Wei Liu Tingting Liu Kaifeng He Zha Jin Meiyi Chen Yuqian Jiang Yuxiao Qian Hongrong Hong Dezhi Zhang Quan Liu Zhengtao Yang Qianyong Li |
| author_facet | Zhengkai Wei Qinqin Jin Wei Liu Tingting Liu Kaifeng He Zha Jin Meiyi Chen Yuqian Jiang Yuxiao Qian Hongrong Hong Dezhi Zhang Quan Liu Zhengtao Yang Qianyong Li |
| author_sort | Zhengkai Wei |
| collection | DOAJ |
| description | ABSTRACT: Gliotoxin (GT) belongs to the epipolythiodioxopiperazine (ETP) family, which is considered a crucial virulence determinant among the secondary metabolites produced by Aspergillus fumigatus. The metabolites are commonly found in food and feed, contributing to the invasion and immune escape of Aspergillus fumigatus, thereby posing a significant threat to the health of livestock, poultry, and humans. Heterophil extracellular traps (HETs), a novel form of innate immune defense, have been documented in the chicken's innate immune systems for capturing and eliminating invading microbes. However, the effects and mechanisms of GT on the production of duck HETs in vitro remain unknown. In this study, we first confirmed the presence of HETs in duck innate immune systems and further investigated the molecular mechanism underlying GT-induced HETs release. Our results demonstrate that GT can trigger typical release of HETs in duck. The structures of GT-induced HETs structures were characterized by DNA decoration, citrullinated histones 3, and elastase. Furthermore, NADPH oxidase, glycolysis, ERK1/2 and p38 signaling pathway were found to regulate GT-induced HETs. In summary, our findings reveal that gliotoxin activates HETs release in the early innate immune system of duck while providing new insights into the immunotoxicity of GT towards ducks. |
| first_indexed | 2024-04-24T07:05:42Z |
| format | Article |
| id | doaj.art-b1588b0325af437890c65fdcf8610b06 |
| institution | Directory Open Access Journal |
| issn | 0032-5791 |
| language | English |
| last_indexed | 2024-04-24T07:05:42Z |
| publishDate | 2024-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Poultry Science |
| spelling | doaj.art-b1588b0325af437890c65fdcf8610b062024-04-22T04:11:28ZengElsevierPoultry Science0032-57912024-06-011036103717Gliotoxin elicits immunotoxicity in the early innate immune system of ducksZhengkai Wei0Qinqin Jin1Wei Liu2Tingting Liu3Kaifeng He4Zha Jin5Meiyi Chen6Yuqian Jiang7Yuxiao Qian8Hongrong Hong9Dezhi Zhang10Quan Liu11Zhengtao Yang12Qianyong Li13College of Veterinary Medicine, Southwest University, Chongqing, 400715, PR China; Corresponding author.College of Life Sciences and Engineering, Foshan University, Foshan 528225, Guangdong Province, PR ChinaCollege of Life Sciences and Engineering, Foshan University, Foshan 528225, Guangdong Province, PR ChinaCollege of Life Sciences and Engineering, Foshan University, Foshan 528225, Guangdong Province, PR ChinaCollege of Veterinary Medicine, Southwest University, Chongqing, 400715, PR ChinaCollege of Life Sciences and Engineering, Foshan University, Foshan 528225, Guangdong Province, PR ChinaCollege of Life Sciences and Engineering, Foshan University, Foshan 528225, Guangdong Province, PR ChinaCollege of Life Sciences and Engineering, Foshan University, Foshan 528225, Guangdong Province, PR ChinaCollege of Life Sciences and Engineering, Foshan University, Foshan 528225, Guangdong Province, PR ChinaCollege of Life Sciences and Engineering, Foshan University, Foshan 528225, Guangdong Province, PR ChinaCollege of Veterinary Medicine, Southwest University, Chongqing, 400715, PR ChinaCollege of Life Sciences and Engineering, Foshan University, Foshan 528225, Guangdong Province, PR ChinaCollege of Life Sciences and Engineering, Foshan University, Foshan 528225, Guangdong Province, PR ChinaCollege of Veterinary Medicine, Southwest University, Chongqing, 400715, PR ChinaABSTRACT: Gliotoxin (GT) belongs to the epipolythiodioxopiperazine (ETP) family, which is considered a crucial virulence determinant among the secondary metabolites produced by Aspergillus fumigatus. The metabolites are commonly found in food and feed, contributing to the invasion and immune escape of Aspergillus fumigatus, thereby posing a significant threat to the health of livestock, poultry, and humans. Heterophil extracellular traps (HETs), a novel form of innate immune defense, have been documented in the chicken's innate immune systems for capturing and eliminating invading microbes. However, the effects and mechanisms of GT on the production of duck HETs in vitro remain unknown. In this study, we first confirmed the presence of HETs in duck innate immune systems and further investigated the molecular mechanism underlying GT-induced HETs release. Our results demonstrate that GT can trigger typical release of HETs in duck. The structures of GT-induced HETs structures were characterized by DNA decoration, citrullinated histones 3, and elastase. Furthermore, NADPH oxidase, glycolysis, ERK1/2 and p38 signaling pathway were found to regulate GT-induced HETs. In summary, our findings reveal that gliotoxin activates HETs release in the early innate immune system of duck while providing new insights into the immunotoxicity of GT towards ducks.http://www.sciencedirect.com/science/article/pii/S0032579124002980gliotoxinduck heterophilglycolysisimmunotoxicity |
| spellingShingle | Zhengkai Wei Qinqin Jin Wei Liu Tingting Liu Kaifeng He Zha Jin Meiyi Chen Yuqian Jiang Yuxiao Qian Hongrong Hong Dezhi Zhang Quan Liu Zhengtao Yang Qianyong Li Gliotoxin elicits immunotoxicity in the early innate immune system of ducks Poultry Science gliotoxin duck heterophil glycolysis immunotoxicity |
| title | Gliotoxin elicits immunotoxicity in the early innate immune system of ducks |
| title_full | Gliotoxin elicits immunotoxicity in the early innate immune system of ducks |
| title_fullStr | Gliotoxin elicits immunotoxicity in the early innate immune system of ducks |
| title_full_unstemmed | Gliotoxin elicits immunotoxicity in the early innate immune system of ducks |
| title_short | Gliotoxin elicits immunotoxicity in the early innate immune system of ducks |
| title_sort | gliotoxin elicits immunotoxicity in the early innate immune system of ducks |
| topic | gliotoxin duck heterophil glycolysis immunotoxicity |
| url | http://www.sciencedirect.com/science/article/pii/S0032579124002980 |
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