Proteomic Profiling of Extracellular Vesicles Isolated from Plasma and Peritoneal Exudate in Mice Induced by <i>Crotalus scutulatus scutulatus</i> Crude Venom and Its Purified Cysteine-Rich Secretory Protein (Css-CRiSP)
Increased vascular permeability is a frequent outcome of viperid snakebite envenomation, leading to local and systemic complications. We reported that snake venom cysteine-rich secretory proteins (svCRiSPs) from North American pit vipers increase vascular permeability both in vitro and in vivo. They...
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| Format: | Article |
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MDPI AG
2023-07-01
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| Series: | Toxins |
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| Online Access: | https://www.mdpi.com/2072-6651/15/7/434 |
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| author | Armando Reyes Joseph D. Hatcher Emelyn Salazar Jacob Galan Anton Iliuk Elda E. Sanchez Montamas Suntravat |
| author_facet | Armando Reyes Joseph D. Hatcher Emelyn Salazar Jacob Galan Anton Iliuk Elda E. Sanchez Montamas Suntravat |
| author_sort | Armando Reyes |
| collection | DOAJ |
| description | Increased vascular permeability is a frequent outcome of viperid snakebite envenomation, leading to local and systemic complications. We reported that snake venom cysteine-rich secretory proteins (svCRiSPs) from North American pit vipers increase vascular permeability both in vitro and in vivo. They also induce acute activation of several adhesion and signaling molecules that may play a critical role in the pathophysiology of snakebites. Extracellular vesicles (EVs) have gained interest for their diverse functions in intercellular communication, regulating cellular processes, blood-endothelium interactions, vascular permeability, and immune modulation. They also hold potential as valuable biomarkers for diagnosing, predicting, and monitoring therapeutic responses in different diseases. This study aimed to identify proteins in peritoneal exudate and plasma EVs isolated from BALB/c mice following a 30 min post-injection of <i>Crotalus scutulatus scutulatus</i> venom and its purified CRiSP (Css-CRiSP). EVs were isolated from these biofluids using the EVtrap method. Proteomic analysis of exudate- and plasma-derived EVs was performed using LC-MS/MS. We observed significant upregulation or downregulation of proteins involved in cell adhesion, cytoskeleton rearrangement, signal transduction, immune responses, and vesicle-mediated transports. These findings suggest that svCRiSPs play a crucial role in the acute effects of venom and contribute to the local and systemic toxicity of snakebites. |
| first_indexed | 2024-03-11T00:35:28Z |
| format | Article |
| id | doaj.art-b15adcb650ec47ca842273a284517ded |
| institution | Directory Open Access Journal |
| issn | 2072-6651 |
| language | English |
| last_indexed | 2024-03-11T00:35:28Z |
| publishDate | 2023-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Toxins |
| spelling | doaj.art-b15adcb650ec47ca842273a284517ded2023-11-18T21:38:08ZengMDPI AGToxins2072-66512023-07-0115743410.3390/toxins15070434Proteomic Profiling of Extracellular Vesicles Isolated from Plasma and Peritoneal Exudate in Mice Induced by <i>Crotalus scutulatus scutulatus</i> Crude Venom and Its Purified Cysteine-Rich Secretory Protein (Css-CRiSP)Armando Reyes0Joseph D. Hatcher1Emelyn Salazar2Jacob Galan3Anton Iliuk4Elda E. Sanchez5Montamas Suntravat6National Natural Toxins Research Center (NNTRC), Texas A&M University-Kingsville, MSC 224, 975 West Avenue B, Kingsville, TX 78363, USANational Natural Toxins Research Center (NNTRC), Texas A&M University-Kingsville, MSC 224, 975 West Avenue B, Kingsville, TX 78363, USANational Natural Toxins Research Center (NNTRC), Texas A&M University-Kingsville, MSC 224, 975 West Avenue B, Kingsville, TX 78363, USADepartment of Human Genetics, The University of Texas Rio Grande Valley School of Medicine, Brownsville, TX 78539, USATymora Analytical Operations, West Lafayette, IN 47906, USANational Natural Toxins Research Center (NNTRC), Texas A&M University-Kingsville, MSC 224, 975 West Avenue B, Kingsville, TX 78363, USANational Natural Toxins Research Center (NNTRC), Texas A&M University-Kingsville, MSC 224, 975 West Avenue B, Kingsville, TX 78363, USAIncreased vascular permeability is a frequent outcome of viperid snakebite envenomation, leading to local and systemic complications. We reported that snake venom cysteine-rich secretory proteins (svCRiSPs) from North American pit vipers increase vascular permeability both in vitro and in vivo. They also induce acute activation of several adhesion and signaling molecules that may play a critical role in the pathophysiology of snakebites. Extracellular vesicles (EVs) have gained interest for their diverse functions in intercellular communication, regulating cellular processes, blood-endothelium interactions, vascular permeability, and immune modulation. They also hold potential as valuable biomarkers for diagnosing, predicting, and monitoring therapeutic responses in different diseases. This study aimed to identify proteins in peritoneal exudate and plasma EVs isolated from BALB/c mice following a 30 min post-injection of <i>Crotalus scutulatus scutulatus</i> venom and its purified CRiSP (Css-CRiSP). EVs were isolated from these biofluids using the EVtrap method. Proteomic analysis of exudate- and plasma-derived EVs was performed using LC-MS/MS. We observed significant upregulation or downregulation of proteins involved in cell adhesion, cytoskeleton rearrangement, signal transduction, immune responses, and vesicle-mediated transports. These findings suggest that svCRiSPs play a crucial role in the acute effects of venom and contribute to the local and systemic toxicity of snakebites.https://www.mdpi.com/2072-6651/15/7/434snakebitesvascular permeabilitysnake venom cysteine-rich secretory proteins (svCRiSPs)extracellular vehicles (EVs)proteomic analysis |
| spellingShingle | Armando Reyes Joseph D. Hatcher Emelyn Salazar Jacob Galan Anton Iliuk Elda E. Sanchez Montamas Suntravat Proteomic Profiling of Extracellular Vesicles Isolated from Plasma and Peritoneal Exudate in Mice Induced by <i>Crotalus scutulatus scutulatus</i> Crude Venom and Its Purified Cysteine-Rich Secretory Protein (Css-CRiSP) Toxins snakebites vascular permeability snake venom cysteine-rich secretory proteins (svCRiSPs) extracellular vehicles (EVs) proteomic analysis |
| title | Proteomic Profiling of Extracellular Vesicles Isolated from Plasma and Peritoneal Exudate in Mice Induced by <i>Crotalus scutulatus scutulatus</i> Crude Venom and Its Purified Cysteine-Rich Secretory Protein (Css-CRiSP) |
| title_full | Proteomic Profiling of Extracellular Vesicles Isolated from Plasma and Peritoneal Exudate in Mice Induced by <i>Crotalus scutulatus scutulatus</i> Crude Venom and Its Purified Cysteine-Rich Secretory Protein (Css-CRiSP) |
| title_fullStr | Proteomic Profiling of Extracellular Vesicles Isolated from Plasma and Peritoneal Exudate in Mice Induced by <i>Crotalus scutulatus scutulatus</i> Crude Venom and Its Purified Cysteine-Rich Secretory Protein (Css-CRiSP) |
| title_full_unstemmed | Proteomic Profiling of Extracellular Vesicles Isolated from Plasma and Peritoneal Exudate in Mice Induced by <i>Crotalus scutulatus scutulatus</i> Crude Venom and Its Purified Cysteine-Rich Secretory Protein (Css-CRiSP) |
| title_short | Proteomic Profiling of Extracellular Vesicles Isolated from Plasma and Peritoneal Exudate in Mice Induced by <i>Crotalus scutulatus scutulatus</i> Crude Venom and Its Purified Cysteine-Rich Secretory Protein (Css-CRiSP) |
| title_sort | proteomic profiling of extracellular vesicles isolated from plasma and peritoneal exudate in mice induced by i crotalus scutulatus scutulatus i crude venom and its purified cysteine rich secretory protein css crisp |
| topic | snakebites vascular permeability snake venom cysteine-rich secretory proteins (svCRiSPs) extracellular vehicles (EVs) proteomic analysis |
| url | https://www.mdpi.com/2072-6651/15/7/434 |
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