An easy and robust method of preparation of capsules for delivering probiotic bacteria by a 3D bioprinting
Probiotic bacteria such as Lactobacillus rhamnosus GG (LGG) play an important role in maintaining a healthy microbiota environment. Oral administration is the only way to deliver probiotic bacteria to the small intestine. Due to the harsh environment of the gastrointestinal tract, only a small propo...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2022-12-01
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| Series: | Food Hydrocolloids for Health |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2667025922000358 |
| _version_ | 1811315609055526912 |
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| author | Abhik Mallick Fabienne Quilès Grégory Francius Jennifer Burgain Claire Gaiani Joël Scher Sawsan Amara Cécile Lemaitre Philippe Marchal Halima Alem |
| author_facet | Abhik Mallick Fabienne Quilès Grégory Francius Jennifer Burgain Claire Gaiani Joël Scher Sawsan Amara Cécile Lemaitre Philippe Marchal Halima Alem |
| author_sort | Abhik Mallick |
| collection | DOAJ |
| description | Probiotic bacteria such as Lactobacillus rhamnosus GG (LGG) play an important role in maintaining a healthy microbiota environment. Oral administration is the only way to deliver probiotic bacteria to the small intestine. Due to the harsh environment of the gastrointestinal tract, only a small proportion of probiotic bacteria can reach the target site, limiting their beneficial effects. Although many encapsulation techniques exist, the loss of bacterial cell viability, high cost, and low payload remain a major problem. In this work, we present a state-of-the-art microextrusion technique for encapsulating probiotic bacteria using a 3D bio-printer. Using a composite of sodium alginate, gelatin gel and LGG as bioink, we printed encapsulated LGG bacteria that were stable for 7 days. The production of the encapsulated bacteria was a very fast and simple process. We printed 120 probiotic capsules, each containing 108 CFU/ml, within 30 min. On microscopic examination of the LGG capsules, the encapsulated LGG bacteria were evenly distributed in the alginate-gelatin hydrogel. The bacteria in the capsules were viable and alive for 7 days at 4 °C and survived the harsh gastrointestinal fluid environment in vitro. This bioprinting method for encapsulating probiotic bacteria for their specific delivery in the gut could become a revolutionary method for industrial applications. |
| first_indexed | 2024-04-13T11:33:20Z |
| format | Article |
| id | doaj.art-b15e38239e5e4e5c96703fc57db76e36 |
| institution | Directory Open Access Journal |
| issn | 2667-0259 |
| language | English |
| last_indexed | 2024-04-13T11:33:20Z |
| publishDate | 2022-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Food Hydrocolloids for Health |
| spelling | doaj.art-b15e38239e5e4e5c96703fc57db76e362022-12-22T02:48:31ZengElsevierFood Hydrocolloids for Health2667-02592022-12-012100088An easy and robust method of preparation of capsules for delivering probiotic bacteria by a 3D bioprintingAbhik Mallick0Fabienne Quilès1Grégory Francius2Jennifer Burgain3Claire Gaiani4Joël Scher5Sawsan Amara6Cécile Lemaitre7Philippe Marchal8Halima Alem9Université de Lorraine, CNRS, IJL, F-54000 Nancy, FranceUniversité de Lorraine, CNRS, LCPME, F-54600 Nancy, FranceUniversité de Lorraine, CNRS, LCPME, F-54600 Nancy, FranceUniversité de Lorraine, LIBio, F-54000, FranceUniversité de Lorraine, LIBio, F-54000, FranceUniversité de Lorraine, LIBio, F-54000, FranceLipolytech, Zone Luminy Biotech, 163 avenue de Luminy, 13288 Marseille Cedex 09, FranceUniversité de Lorraine, LRGP, F-54000, FranceUniversité de Lorraine, LRGP, F-54000, FranceUniversité de Lorraine, CNRS, IJL, F-54000 Nancy, France; Institut Universitaire de France, France; Corresponding author.Probiotic bacteria such as Lactobacillus rhamnosus GG (LGG) play an important role in maintaining a healthy microbiota environment. Oral administration is the only way to deliver probiotic bacteria to the small intestine. Due to the harsh environment of the gastrointestinal tract, only a small proportion of probiotic bacteria can reach the target site, limiting their beneficial effects. Although many encapsulation techniques exist, the loss of bacterial cell viability, high cost, and low payload remain a major problem. In this work, we present a state-of-the-art microextrusion technique for encapsulating probiotic bacteria using a 3D bio-printer. Using a composite of sodium alginate, gelatin gel and LGG as bioink, we printed encapsulated LGG bacteria that were stable for 7 days. The production of the encapsulated bacteria was a very fast and simple process. We printed 120 probiotic capsules, each containing 108 CFU/ml, within 30 min. On microscopic examination of the LGG capsules, the encapsulated LGG bacteria were evenly distributed in the alginate-gelatin hydrogel. The bacteria in the capsules were viable and alive for 7 days at 4 °C and survived the harsh gastrointestinal fluid environment in vitro. This bioprinting method for encapsulating probiotic bacteria for their specific delivery in the gut could become a revolutionary method for industrial applications.http://www.sciencedirect.com/science/article/pii/S2667025922000358Probiotics3D bioprintingSurvival in the gastrointestinal fluidsIndustrial applications |
| spellingShingle | Abhik Mallick Fabienne Quilès Grégory Francius Jennifer Burgain Claire Gaiani Joël Scher Sawsan Amara Cécile Lemaitre Philippe Marchal Halima Alem An easy and robust method of preparation of capsules for delivering probiotic bacteria by a 3D bioprinting Food Hydrocolloids for Health Probiotics 3D bioprinting Survival in the gastrointestinal fluids Industrial applications |
| title | An easy and robust method of preparation of capsules for delivering probiotic bacteria by a 3D bioprinting |
| title_full | An easy and robust method of preparation of capsules for delivering probiotic bacteria by a 3D bioprinting |
| title_fullStr | An easy and robust method of preparation of capsules for delivering probiotic bacteria by a 3D bioprinting |
| title_full_unstemmed | An easy and robust method of preparation of capsules for delivering probiotic bacteria by a 3D bioprinting |
| title_short | An easy and robust method of preparation of capsules for delivering probiotic bacteria by a 3D bioprinting |
| title_sort | easy and robust method of preparation of capsules for delivering probiotic bacteria by a 3d bioprinting |
| topic | Probiotics 3D bioprinting Survival in the gastrointestinal fluids Industrial applications |
| url | http://www.sciencedirect.com/science/article/pii/S2667025922000358 |
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