Production, Preservation, and Transfer of South American Camelid Embryos
The current review summarizes progress in the field of in vitro and in vivo production of South American Camelid embryos. Both methods require ovarian superstimulation (with FSH and eCG) to obtain multiple ovulations (in vivo embryo production) or to induce follicle growth for oocyte collection (in...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2017-11-01
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| Series: | Frontiers in Veterinary Science |
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| Online Access: | http://journal.frontiersin.org/article/10.3389/fvets.2017.00190/full |
| _version_ | 1811316635989966848 |
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| author | Virginia L. Trasorras Virginia L. Trasorras María Ignacia Carretero María Ignacia Carretero Deborah M. Neild Maria Graciela Chaves Susana M. Giuliano Marcelo H. Miragaya |
| author_facet | Virginia L. Trasorras Virginia L. Trasorras María Ignacia Carretero María Ignacia Carretero Deborah M. Neild Maria Graciela Chaves Susana M. Giuliano Marcelo H. Miragaya |
| author_sort | Virginia L. Trasorras |
| collection | DOAJ |
| description | The current review summarizes progress in the field of in vitro and in vivo production of South American Camelid embryos. Both methods require ovarian superstimulation (with FSH and eCG) to obtain multiple ovulations (in vivo embryo production) or to induce follicle growth for oocyte collection (in vitro embryo production). Moreover, superstimulation entails prior administration of hormones that inhibit follicular growth (progesterone, progestagens, and estrogens). Cumulus-oocyte complexes obtained must mature in vivo (buserelin administration) or in vitro to then be subjected to in vitro fertilization or intracytoplasmic sperm injection. All these techniques also require morphologically normal, motile spermatozoa to achieve fertilization. Methods used to decrease semen viscosity and to select the best spermatozoa (Percoll®; Androcoll-ETM) are described. Additionally, nuclear transfer or cloning has been applied in llamas. Up to now, embryo deep-freezing and vitrification have progressed slowly but are at the height of development. Embryos that are obtained by any of these techniques, either in vivo or in vitro, need to be transferred to synchronized recipient females. The best results are achieved after transfer to the left uterine horn with an ipsilateral ovulation. No live offspring have been obtained after the transfer of cryopreserved embryos. Applying reproductive biotechnologies, such as those described, will permit the expansion of genetically selected animals in the population and also that of wild camelid species, vicunas, and guanacos, whose embryos could then be transferred to the uterus of domestic species. |
| first_indexed | 2024-04-13T11:52:54Z |
| format | Article |
| id | doaj.art-b15ff1807e08461cb4dc8a107141f7c4 |
| institution | Directory Open Access Journal |
| issn | 2297-1769 |
| language | English |
| last_indexed | 2024-04-13T11:52:54Z |
| publishDate | 2017-11-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Veterinary Science |
| spelling | doaj.art-b15ff1807e08461cb4dc8a107141f7c42022-12-22T02:47:59ZengFrontiers Media S.A.Frontiers in Veterinary Science2297-17692017-11-01410.3389/fvets.2017.00190276693Production, Preservation, and Transfer of South American Camelid EmbryosVirginia L. Trasorras0Virginia L. Trasorras1María Ignacia Carretero2María Ignacia Carretero3Deborah M. Neild4Maria Graciela Chaves5Susana M. Giuliano6Marcelo H. Miragaya7Facultad de Ciencias Veterinarias (FCV), Instituto de Investigación y Tecnología en Reproducción Animal (INITRA), Cátedra de Teriogenología, Universidad de Buenos Aires (UBA), Buenos Aires, ArgentinaConsejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Buenos Aires, ArgentinaFacultad de Ciencias Veterinarias (FCV), Instituto de Investigación y Tecnología en Reproducción Animal (INITRA), Cátedra de Teriogenología, Universidad de Buenos Aires (UBA), Buenos Aires, ArgentinaConsejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Buenos Aires, ArgentinaFacultad de Ciencias Veterinarias (FCV), Instituto de Investigación y Tecnología en Reproducción Animal (INITRA), Cátedra de Teriogenología, Universidad de Buenos Aires (UBA), Buenos Aires, ArgentinaFacultad de Ciencias Veterinarias (FCV), Instituto de Investigación y Tecnología en Reproducción Animal (INITRA), Cátedra de Teriogenología, Universidad de Buenos Aires (UBA), Buenos Aires, ArgentinaFacultad de Ciencias Veterinarias (FCV), Instituto de Investigación y Tecnología en Reproducción Animal (INITRA), Cátedra de Física Biológica, Universidad de Buenos Aires (UBA), Buenos Aires, ArgentinaFacultad de Ciencias Veterinarias (FCV), Instituto de Investigación y Tecnología en Reproducción Animal (INITRA), Cátedra de Teriogenología, Universidad de Buenos Aires (UBA), Buenos Aires, ArgentinaThe current review summarizes progress in the field of in vitro and in vivo production of South American Camelid embryos. Both methods require ovarian superstimulation (with FSH and eCG) to obtain multiple ovulations (in vivo embryo production) or to induce follicle growth for oocyte collection (in vitro embryo production). Moreover, superstimulation entails prior administration of hormones that inhibit follicular growth (progesterone, progestagens, and estrogens). Cumulus-oocyte complexes obtained must mature in vivo (buserelin administration) or in vitro to then be subjected to in vitro fertilization or intracytoplasmic sperm injection. All these techniques also require morphologically normal, motile spermatozoa to achieve fertilization. Methods used to decrease semen viscosity and to select the best spermatozoa (Percoll®; Androcoll-ETM) are described. Additionally, nuclear transfer or cloning has been applied in llamas. Up to now, embryo deep-freezing and vitrification have progressed slowly but are at the height of development. Embryos that are obtained by any of these techniques, either in vivo or in vitro, need to be transferred to synchronized recipient females. The best results are achieved after transfer to the left uterine horn with an ipsilateral ovulation. No live offspring have been obtained after the transfer of cryopreserved embryos. Applying reproductive biotechnologies, such as those described, will permit the expansion of genetically selected animals in the population and also that of wild camelid species, vicunas, and guanacos, whose embryos could then be transferred to the uterus of domestic species.http://journal.frontiersin.org/article/10.3389/fvets.2017.00190/fullcamelidsembryoIVPsemenreproductive biotechnologies |
| spellingShingle | Virginia L. Trasorras Virginia L. Trasorras María Ignacia Carretero María Ignacia Carretero Deborah M. Neild Maria Graciela Chaves Susana M. Giuliano Marcelo H. Miragaya Production, Preservation, and Transfer of South American Camelid Embryos Frontiers in Veterinary Science camelids embryo IVP semen reproductive biotechnologies |
| title | Production, Preservation, and Transfer of South American Camelid Embryos |
| title_full | Production, Preservation, and Transfer of South American Camelid Embryos |
| title_fullStr | Production, Preservation, and Transfer of South American Camelid Embryos |
| title_full_unstemmed | Production, Preservation, and Transfer of South American Camelid Embryos |
| title_short | Production, Preservation, and Transfer of South American Camelid Embryos |
| title_sort | production preservation and transfer of south american camelid embryos |
| topic | camelids embryo IVP semen reproductive biotechnologies |
| url | http://journal.frontiersin.org/article/10.3389/fvets.2017.00190/full |
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