High Resolution and Automatable Cytogenetic Biodosimetry Using In Situ Telomere and Centromere Hybridization for the Accurate Detection of DNA Damage: An Overview
In the event of a radiological or nuclear accident, or when physical dosimetry is not available, the scoring of radiation-induced chromosomal aberrations in lymphocytes constitutes an essential tool for the estimation of the absorbed dose of the exposed individual and for effective triage. Cytogenet...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-03-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/24/6/5699 |
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| author | Radhia M’Kacher Bruno Colicchio Steffen Junker Elie El Maalouf Leonhard Heidingsfelder Andreas Plesch Alain Dieterlen Eric Jeandidier Patrice Carde Philippe Voisin |
| author_facet | Radhia M’Kacher Bruno Colicchio Steffen Junker Elie El Maalouf Leonhard Heidingsfelder Andreas Plesch Alain Dieterlen Eric Jeandidier Patrice Carde Philippe Voisin |
| author_sort | Radhia M’Kacher |
| collection | DOAJ |
| description | In the event of a radiological or nuclear accident, or when physical dosimetry is not available, the scoring of radiation-induced chromosomal aberrations in lymphocytes constitutes an essential tool for the estimation of the absorbed dose of the exposed individual and for effective triage. Cytogenetic biodosimetry employs different cytogenetic assays including the scoring of dicentrics, micronuclei, and translocations as well as analyses of induced premature chromosome condensation to define the frequency of chromosome aberrations. However, inherent challenges using these techniques include the considerable time span from sampling to result, the sensitivity and specificity of the various techniques, and the requirement of highly skilled personnel. Thus, techniques that obviate these challenges are needed. The introduction of telomere and centromere (TC) staining have successfully met these challenges and, in addition, greatly improved the efficiency of cytogenetic biodosimetry through the development of automated approaches, thus reducing the need for specialized personnel. Here, we review the role of the various cytogenetic dosimeters and their recent improvements in the management of populations exposed to genotoxic agents such as ionizing radiation. Finally, we discuss the emerging potentials to exploit these techniques in a wider spectrum of medical and biological applications, e.g., in cancer biology to identify prognostic biomarkers for the optimal triage and treatment of patients. |
| first_indexed | 2024-03-11T06:25:18Z |
| format | Article |
| id | doaj.art-b161973347de42a1ba6d74da351d203e |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-11T06:25:18Z |
| publishDate | 2023-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-b161973347de42a1ba6d74da351d203e2023-11-17T11:37:45ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-03-01246569910.3390/ijms24065699High Resolution and Automatable Cytogenetic Biodosimetry Using In Situ Telomere and Centromere Hybridization for the Accurate Detection of DNA Damage: An OverviewRadhia M’Kacher0Bruno Colicchio1Steffen Junker2Elie El Maalouf3Leonhard Heidingsfelder4Andreas Plesch5Alain Dieterlen6Eric Jeandidier7Patrice Carde8Philippe Voisin9Cell Environment DNA Damage R&D, Genopole, 91000 Evry-Courcouronnes, FranceIRIMAS, Institut de Recherche en Informatique, Mathématiques, Automatique et Signal, Université de Haute-Alsace, 69093 Mulhouse, FranceInstitute of Biomedicine, University of Aarhus, DK-8000 Aarhus, DenmarkCell Environment DNA Damage R&D, Genopole, 91000 Evry-Courcouronnes, FranceMetaSystems GmbH, Robert-Bosch-Str. 6, D-68804 Altlussheim, GermanyMetaSystems GmbH, Robert-Bosch-Str. 6, D-68804 Altlussheim, GermanyIRIMAS, Institut de Recherche en Informatique, Mathématiques, Automatique et Signal, Université de Haute-Alsace, 69093 Mulhouse, FranceLaboratoire de Génétique, Groupe Hospitalier de la Région de Mulhouse Sud-Alsace, 69093 Mulhouse, FranceDepartment of Hematology, Institut Gustave Roussy, 94804 Villejuif, FranceCell Environment DNA Damage R&D, Genopole, 91000 Evry-Courcouronnes, FranceIn the event of a radiological or nuclear accident, or when physical dosimetry is not available, the scoring of radiation-induced chromosomal aberrations in lymphocytes constitutes an essential tool for the estimation of the absorbed dose of the exposed individual and for effective triage. Cytogenetic biodosimetry employs different cytogenetic assays including the scoring of dicentrics, micronuclei, and translocations as well as analyses of induced premature chromosome condensation to define the frequency of chromosome aberrations. However, inherent challenges using these techniques include the considerable time span from sampling to result, the sensitivity and specificity of the various techniques, and the requirement of highly skilled personnel. Thus, techniques that obviate these challenges are needed. The introduction of telomere and centromere (TC) staining have successfully met these challenges and, in addition, greatly improved the efficiency of cytogenetic biodosimetry through the development of automated approaches, thus reducing the need for specialized personnel. Here, we review the role of the various cytogenetic dosimeters and their recent improvements in the management of populations exposed to genotoxic agents such as ionizing radiation. Finally, we discuss the emerging potentials to exploit these techniques in a wider spectrum of medical and biological applications, e.g., in cancer biology to identify prognostic biomarkers for the optimal triage and treatment of patients.https://www.mdpi.com/1422-0067/24/6/5699cytogenetic biodosimetryradioprotectiontelomerecentromerechromosomal instability |
| spellingShingle | Radhia M’Kacher Bruno Colicchio Steffen Junker Elie El Maalouf Leonhard Heidingsfelder Andreas Plesch Alain Dieterlen Eric Jeandidier Patrice Carde Philippe Voisin High Resolution and Automatable Cytogenetic Biodosimetry Using In Situ Telomere and Centromere Hybridization for the Accurate Detection of DNA Damage: An Overview International Journal of Molecular Sciences cytogenetic biodosimetry radioprotection telomere centromere chromosomal instability |
| title | High Resolution and Automatable Cytogenetic Biodosimetry Using In Situ Telomere and Centromere Hybridization for the Accurate Detection of DNA Damage: An Overview |
| title_full | High Resolution and Automatable Cytogenetic Biodosimetry Using In Situ Telomere and Centromere Hybridization for the Accurate Detection of DNA Damage: An Overview |
| title_fullStr | High Resolution and Automatable Cytogenetic Biodosimetry Using In Situ Telomere and Centromere Hybridization for the Accurate Detection of DNA Damage: An Overview |
| title_full_unstemmed | High Resolution and Automatable Cytogenetic Biodosimetry Using In Situ Telomere and Centromere Hybridization for the Accurate Detection of DNA Damage: An Overview |
| title_short | High Resolution and Automatable Cytogenetic Biodosimetry Using In Situ Telomere and Centromere Hybridization for the Accurate Detection of DNA Damage: An Overview |
| title_sort | high resolution and automatable cytogenetic biodosimetry using in situ telomere and centromere hybridization for the accurate detection of dna damage an overview |
| topic | cytogenetic biodosimetry radioprotection telomere centromere chromosomal instability |
| url | https://www.mdpi.com/1422-0067/24/6/5699 |
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