Anti-Inflammatory Activity of Fucoidan Extracts In Vitro
Fucoidans are sulfated, complex, fucose-rich polymers found in brown seaweeds. Fucoidans have been shown to have multiple bioactivities, including anti-inflammatory effects, and are known to inhibit inflammatory processes via a number of pathways such as selectin blockade and enzyme inhibition, and...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-12-01
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| Series: | Marine Drugs |
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| Online Access: | https://www.mdpi.com/1660-3397/19/12/702 |
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| author | Tauseef Ahmad Mathew Suji Eapen Muhammad Ishaq Ah Young Park Samuel S. Karpiniec Damien N. Stringer Sukhwinder Singh Sohal J. Helen Fitton Nuri Guven Vanni Caruso Rajaraman Eri |
| author_facet | Tauseef Ahmad Mathew Suji Eapen Muhammad Ishaq Ah Young Park Samuel S. Karpiniec Damien N. Stringer Sukhwinder Singh Sohal J. Helen Fitton Nuri Guven Vanni Caruso Rajaraman Eri |
| author_sort | Tauseef Ahmad |
| collection | DOAJ |
| description | Fucoidans are sulfated, complex, fucose-rich polymers found in brown seaweeds. Fucoidans have been shown to have multiple bioactivities, including anti-inflammatory effects, and are known to inhibit inflammatory processes via a number of pathways such as selectin blockade and enzyme inhibition, and have demonstrated inhibition of inflammatory pathologies <i>in vivo</i>. In this current investigation, fucoidan extracts from <i>Undaria pinnatifida, Fucus vesiculosus, Macrocystis pyrifera, Ascophyllum nodosum,</i> and <i>Laminaria japonica</i> were assessed for modulation of pro-inflammatory cytokine production (TNF-α, IL-1β, and IL-6) by human peripheral blood mononuclear cells (PBMCs) and in a human macrophage line (THP-1). Fucoidan extracts exhibited no signs of cytotoxicity in THP-1 cells after incubation of 48 h. Additionally, all fucoidan extracts reduced cytokine production in LPS stimulated PBMCs and human THP-1 cells in a dose-dependent fashion. Notably, the 5–30 kDa subfraction from <i>Macrocystis pyrifera</i> was a highly effective inhibitor at lower concentrations. Fucoidan extracts from all species had significant anti-inflammatory effects, but the lowest molecular weight subfractions had maximal effects at low concentrations. These observations on various fucoidan extracts offer insight into strategies that improve their efficacy against inflammation-related pathology. Further studies should be conducted to elucidate the mechanism of action of these extracts. |
| first_indexed | 2024-03-10T03:41:18Z |
| format | Article |
| id | doaj.art-b16225ab59f543ba810a1f7b2556a1a4 |
| institution | Directory Open Access Journal |
| issn | 1660-3397 |
| language | English |
| last_indexed | 2024-03-10T03:41:18Z |
| publishDate | 2021-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Marine Drugs |
| spelling | doaj.art-b16225ab59f543ba810a1f7b2556a1a42023-11-23T09:18:28ZengMDPI AGMarine Drugs1660-33972021-12-01191270210.3390/md19120702Anti-Inflammatory Activity of Fucoidan Extracts In VitroTauseef Ahmad0Mathew Suji Eapen1Muhammad Ishaq2Ah Young Park3Samuel S. Karpiniec4Damien N. Stringer5Sukhwinder Singh Sohal6J. Helen Fitton7Nuri Guven8Vanni Caruso9Rajaraman Eri10School of Health Sciences, University of Tasmania, Launceston, TAS 7248, AustraliaRespiratory Translational Research Group, Department of Laboratory Medicine, School of Health Sciences, College of Health and Medicine, University of Tasmania, Launceston, TAS 7248, AustraliaSchool of Pharmacy and Pharmacology, University of Tasmania, Hobart, TAS 7005, AustraliaMarinova Pty Ltd., Cambridge, TAS 7170, AustraliaMarinova Pty Ltd., Cambridge, TAS 7170, AustraliaMarinova Pty Ltd., Cambridge, TAS 7170, AustraliaRespiratory Translational Research Group, Department of Laboratory Medicine, School of Health Sciences, College of Health and Medicine, University of Tasmania, Launceston, TAS 7248, AustraliaMarinova Pty Ltd., Cambridge, TAS 7170, AustraliaSchool of Pharmacy and Pharmacology, University of Tasmania, Hobart, TAS 7005, AustraliaSchool of Pharmacy and Pharmacology, University of Tasmania, Hobart, TAS 7005, AustraliaSchool of Health Sciences, University of Tasmania, Launceston, TAS 7248, AustraliaFucoidans are sulfated, complex, fucose-rich polymers found in brown seaweeds. Fucoidans have been shown to have multiple bioactivities, including anti-inflammatory effects, and are known to inhibit inflammatory processes via a number of pathways such as selectin blockade and enzyme inhibition, and have demonstrated inhibition of inflammatory pathologies <i>in vivo</i>. In this current investigation, fucoidan extracts from <i>Undaria pinnatifida, Fucus vesiculosus, Macrocystis pyrifera, Ascophyllum nodosum,</i> and <i>Laminaria japonica</i> were assessed for modulation of pro-inflammatory cytokine production (TNF-α, IL-1β, and IL-6) by human peripheral blood mononuclear cells (PBMCs) and in a human macrophage line (THP-1). Fucoidan extracts exhibited no signs of cytotoxicity in THP-1 cells after incubation of 48 h. Additionally, all fucoidan extracts reduced cytokine production in LPS stimulated PBMCs and human THP-1 cells in a dose-dependent fashion. Notably, the 5–30 kDa subfraction from <i>Macrocystis pyrifera</i> was a highly effective inhibitor at lower concentrations. Fucoidan extracts from all species had significant anti-inflammatory effects, but the lowest molecular weight subfractions had maximal effects at low concentrations. These observations on various fucoidan extracts offer insight into strategies that improve their efficacy against inflammation-related pathology. Further studies should be conducted to elucidate the mechanism of action of these extracts.https://www.mdpi.com/1660-3397/19/12/702fucoidanPBMCsTHP-1TNF-α, IL-1β, and IL-6<i>Undaria pinnatifida</i><i>Fucus vesiculosus</i> |
| spellingShingle | Tauseef Ahmad Mathew Suji Eapen Muhammad Ishaq Ah Young Park Samuel S. Karpiniec Damien N. Stringer Sukhwinder Singh Sohal J. Helen Fitton Nuri Guven Vanni Caruso Rajaraman Eri Anti-Inflammatory Activity of Fucoidan Extracts In Vitro Marine Drugs fucoidan PBMCs THP-1 TNF-α, IL-1β, and IL-6 <i>Undaria pinnatifida</i> <i>Fucus vesiculosus</i> |
| title | Anti-Inflammatory Activity of Fucoidan Extracts In Vitro |
| title_full | Anti-Inflammatory Activity of Fucoidan Extracts In Vitro |
| title_fullStr | Anti-Inflammatory Activity of Fucoidan Extracts In Vitro |
| title_full_unstemmed | Anti-Inflammatory Activity of Fucoidan Extracts In Vitro |
| title_short | Anti-Inflammatory Activity of Fucoidan Extracts In Vitro |
| title_sort | anti inflammatory activity of fucoidan extracts in vitro |
| topic | fucoidan PBMCs THP-1 TNF-α, IL-1β, and IL-6 <i>Undaria pinnatifida</i> <i>Fucus vesiculosus</i> |
| url | https://www.mdpi.com/1660-3397/19/12/702 |
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