Transcription and proteome changes involved in re-innervation muscle following nerve crush in rats
Abstract Severe peripheral nerve injury leads to the irreparable disruption of nerve fibers. This leads to disruption of synapses with the designated muscle, which consequently go through progressive atrophy and damage of muscle function. The molecular mechanism that underlies the re-innervation pro...
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| Format: | Article |
| Language: | English |
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BMC
2022-09-01
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| Series: | BMC Genomics |
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| Online Access: | https://doi.org/10.1186/s12864-022-08895-w |
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| author | Haotao Li Wanqiong Yuan Yijian Chen Bofu Lin Shuai Wang Zhantao Deng Qiujian Zheng Qingtian Li |
| author_facet | Haotao Li Wanqiong Yuan Yijian Chen Bofu Lin Shuai Wang Zhantao Deng Qiujian Zheng Qingtian Li |
| author_sort | Haotao Li |
| collection | DOAJ |
| description | Abstract Severe peripheral nerve injury leads to the irreparable disruption of nerve fibers. This leads to disruption of synapses with the designated muscle, which consequently go through progressive atrophy and damage of muscle function. The molecular mechanism that underlies the re-innervation process has yet to be evaluated using proteomics or transcriptomics. In the present study, multi-dimensional data were therefore integrated with transcriptome and proteome profiles in order to investigate the mechanism of re-innervation in muscles. Two simulated nerve injury muscle models in the rat tibial nerve were compared: the nerve was either cut (denervated, DN group) or crushed but with the nerve sheath intact (re-innervated, RN group). The control group had a preserved and intact tibial nerve. At 4 weeks, the RN group showed better tibial nerve function and recovery of muscle atrophy compared to the DN group. As the high expression of Myh3, Postn, Col6a1 and Cfi, the RN group demonstrated superior re-innervation as well. Both differentially expressed genes (DEGs) and proteins (DEPs) were enriched in the peroxisome proliferator-activated receptors (PPARs) signaling pathway, as well as the energy metabolism. This study provides basic information regarding DEGs and DEPs during re-innervation-induced muscle atrophy. Furthermore, the crucial genes and proteins can be detected as possible treatment targets in the future. |
| first_indexed | 2024-04-12T04:28:08Z |
| format | Article |
| id | doaj.art-b1626c3b39344b48a45cfcab66f54289 |
| institution | Directory Open Access Journal |
| issn | 1471-2164 |
| language | English |
| last_indexed | 2024-04-12T04:28:08Z |
| publishDate | 2022-09-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Genomics |
| spelling | doaj.art-b1626c3b39344b48a45cfcab66f542892022-12-22T03:48:00ZengBMCBMC Genomics1471-21642022-09-0123111110.1186/s12864-022-08895-wTranscription and proteome changes involved in re-innervation muscle following nerve crush in ratsHaotao Li0Wanqiong Yuan1Yijian Chen2Bofu Lin3Shuai Wang4Zhantao Deng5Qiujian Zheng6Qingtian Li7Department of Orthopedics, Guangdong Provincial People’s Hospital, Guangdong Academy of Medical SciencesDepartment of Orthopedics, Peking University Third HospitalDepartment of Orthopedics, Shantou Central HospitalDepartment of Orthopedics, Guangdong Provincial People’s Hospital, Guangdong Academy of Medical SciencesDepartment of Orthopedics, Guangdong Provincial People’s Hospital, Guangdong Academy of Medical SciencesDepartment of Orthopedics, Guangdong Provincial People’s Hospital, Guangdong Academy of Medical SciencesDepartment of Orthopedics, Guangdong Provincial People’s Hospital, Guangdong Academy of Medical SciencesDepartment of Orthopedics, Guangdong Provincial People’s Hospital, Guangdong Academy of Medical SciencesAbstract Severe peripheral nerve injury leads to the irreparable disruption of nerve fibers. This leads to disruption of synapses with the designated muscle, which consequently go through progressive atrophy and damage of muscle function. The molecular mechanism that underlies the re-innervation process has yet to be evaluated using proteomics or transcriptomics. In the present study, multi-dimensional data were therefore integrated with transcriptome and proteome profiles in order to investigate the mechanism of re-innervation in muscles. Two simulated nerve injury muscle models in the rat tibial nerve were compared: the nerve was either cut (denervated, DN group) or crushed but with the nerve sheath intact (re-innervated, RN group). The control group had a preserved and intact tibial nerve. At 4 weeks, the RN group showed better tibial nerve function and recovery of muscle atrophy compared to the DN group. As the high expression of Myh3, Postn, Col6a1 and Cfi, the RN group demonstrated superior re-innervation as well. Both differentially expressed genes (DEGs) and proteins (DEPs) were enriched in the peroxisome proliferator-activated receptors (PPARs) signaling pathway, as well as the energy metabolism. This study provides basic information regarding DEGs and DEPs during re-innervation-induced muscle atrophy. Furthermore, the crucial genes and proteins can be detected as possible treatment targets in the future.https://doi.org/10.1186/s12864-022-08895-wRe-innervationDenervationPeripheral nerve injurySkeletal muscle atrophyTranscriptomicsProteomics |
| spellingShingle | Haotao Li Wanqiong Yuan Yijian Chen Bofu Lin Shuai Wang Zhantao Deng Qiujian Zheng Qingtian Li Transcription and proteome changes involved in re-innervation muscle following nerve crush in rats BMC Genomics Re-innervation Denervation Peripheral nerve injury Skeletal muscle atrophy Transcriptomics Proteomics |
| title | Transcription and proteome changes involved in re-innervation muscle following nerve crush in rats |
| title_full | Transcription and proteome changes involved in re-innervation muscle following nerve crush in rats |
| title_fullStr | Transcription and proteome changes involved in re-innervation muscle following nerve crush in rats |
| title_full_unstemmed | Transcription and proteome changes involved in re-innervation muscle following nerve crush in rats |
| title_short | Transcription and proteome changes involved in re-innervation muscle following nerve crush in rats |
| title_sort | transcription and proteome changes involved in re innervation muscle following nerve crush in rats |
| topic | Re-innervation Denervation Peripheral nerve injury Skeletal muscle atrophy Transcriptomics Proteomics |
| url | https://doi.org/10.1186/s12864-022-08895-w |
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