Compound mycotoxin detoxifier alleviating aflatoxin B1 toxic effects on broiler growth performance, organ damage and gut microbiota
ABSTRACT: The aim of this study was to evaluate the effects of compound mycotoxin detoxifier (CMD) on alleviating the toxic effect of aflatoxin B1 (AFB1) for broiler growth performance. One-kilogram CMD consists of 667 g aflatoxin B1-degrading enzyme (ADE, 1,467 U/g), 200 g montmorillonite and 133 g...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-03-01
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| Series: | Poultry Science |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0032579122007283 |
| _version_ | 1811162756498325504 |
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| author | Hongwei Guo Ping Wang Chaoqi Liu Juan Chang Qingqiang Yin Lijun Wang Sanjun Jin Qun Zhu Fushan Lu |
| author_facet | Hongwei Guo Ping Wang Chaoqi Liu Juan Chang Qingqiang Yin Lijun Wang Sanjun Jin Qun Zhu Fushan Lu |
| author_sort | Hongwei Guo |
| collection | DOAJ |
| description | ABSTRACT: The aim of this study was to evaluate the effects of compound mycotoxin detoxifier (CMD) on alleviating the toxic effect of aflatoxin B1 (AFB1) for broiler growth performance. One-kilogram CMD consists of 667 g aflatoxin B1-degrading enzyme (ADE, 1,467 U/g), 200 g montmorillonite and 133 g compound probiotics (CP). The feeding experiment was divided into 2 stages (1–21 d and 22–42 d). In the early stage, a total of 300 one-day-old Ross broilers were randomly divided into 6 groups, 5 replications for each group, 10 broilers (half male and half female) in each replication. In the later feeding stage, about 240 twenty-two-day-old Ross broilers were randomly divided into 6 groups, 8 replications for each group, 5 broilers in each replication. Group A: basal diet; group B: basal diet with 40 μg/kg AFB1; group C: basal diet with 1 g/kg CMD; groups D, E, and F: basal diet with 40 μg/kg AFB1 plus 0.5, 1.0 and 1.5 g/kg CMD, respectively. The results indicated that AFB1 significantly decreased average daily gain (ADG), protein metabolic rate, organ index of thymus, bursa of Fabricius (BF), superoxide dismutase (SOD), glutathione peroxidase (GSH-PX) and catalase activities in serum, and increased AFB1 residues in serum and liver (P < 0.05). Hematoxylin-Eosin (HE) staining analysis of jejunum, liver and kidney showed that AFB1 caused the main pathological changes with different degrees of inflammatory cell infiltration. However, CMD additions could alleviate the negative effects of AFB1 on the above parameters. The gut microbiota analysis indicated that AFB1 could significantly increase the abundances of Staphylococcus-xylosu, Esherichia-coli-g-Escherichia-Shigella, and decrease Lactobacillus-aviarius abundance (P < 0.05), but which were adjusted to almost the same levels as the control group by CMD addition. The correlative analysis showed that Lactobacillus-aviarius abundance was positively correlated with ADG, SOD and BF (P < 0.05), whereas Staphylococcus-xylosus abundance was positively correlated with AFB1 residues in serum and liver (P < 0.05). In conclusion, CMD could keep gut microbiota stable, alleviate histological lesions, increase growth performance, and reduce mycotoxin toxicity. The optimal CMD addition should be 1 g/kg in AFB1-contaminated broilers diet. |
| first_indexed | 2024-04-10T06:35:26Z |
| format | Article |
| id | doaj.art-b1637ce8f11744ab9f76019ce1a21829 |
| institution | Directory Open Access Journal |
| issn | 0032-5791 |
| language | English |
| last_indexed | 2024-04-10T06:35:26Z |
| publishDate | 2023-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Poultry Science |
| spelling | doaj.art-b1637ce8f11744ab9f76019ce1a218292023-03-01T04:30:20ZengElsevierPoultry Science0032-57912023-03-011023102434Compound mycotoxin detoxifier alleviating aflatoxin B1 toxic effects on broiler growth performance, organ damage and gut microbiotaHongwei Guo0Ping Wang1Chaoqi Liu2Juan Chang3Qingqiang Yin4Lijun Wang5Sanjun Jin6Qun Zhu7Fushan Lu8College of Biology and Food Engineering, Huanghuai University, Zhumadian 463000, China; Affiliated Central Hospital of Huanghuai University, Zhumadian 463000, ChinaCollege of Animal Science and Technology, Henan Agricultural University, Zhengzhou 450046, ChinaCollege of Animal Science and Technology, Henan Agricultural University, Zhengzhou 450046, ChinaCollege of Animal Science and Technology, Henan Agricultural University, Zhengzhou 450046, ChinaCollege of Animal Science and Technology, Henan Agricultural University, Zhengzhou 450046, China; Corresponding author:College of Animal Science and Technology, Henan Agricultural University, Zhengzhou 450046, ChinaCollege of Animal Science and Technology, Henan Agricultural University, Zhengzhou 450046, ChinaHenan Delin Biological Product Co. Ltd., Xinxiang 453000, ChinaHenan Puai Feed Co., Ltd., Zhoukou, 466000, ChinaABSTRACT: The aim of this study was to evaluate the effects of compound mycotoxin detoxifier (CMD) on alleviating the toxic effect of aflatoxin B1 (AFB1) for broiler growth performance. One-kilogram CMD consists of 667 g aflatoxin B1-degrading enzyme (ADE, 1,467 U/g), 200 g montmorillonite and 133 g compound probiotics (CP). The feeding experiment was divided into 2 stages (1–21 d and 22–42 d). In the early stage, a total of 300 one-day-old Ross broilers were randomly divided into 6 groups, 5 replications for each group, 10 broilers (half male and half female) in each replication. In the later feeding stage, about 240 twenty-two-day-old Ross broilers were randomly divided into 6 groups, 8 replications for each group, 5 broilers in each replication. Group A: basal diet; group B: basal diet with 40 μg/kg AFB1; group C: basal diet with 1 g/kg CMD; groups D, E, and F: basal diet with 40 μg/kg AFB1 plus 0.5, 1.0 and 1.5 g/kg CMD, respectively. The results indicated that AFB1 significantly decreased average daily gain (ADG), protein metabolic rate, organ index of thymus, bursa of Fabricius (BF), superoxide dismutase (SOD), glutathione peroxidase (GSH-PX) and catalase activities in serum, and increased AFB1 residues in serum and liver (P < 0.05). Hematoxylin-Eosin (HE) staining analysis of jejunum, liver and kidney showed that AFB1 caused the main pathological changes with different degrees of inflammatory cell infiltration. However, CMD additions could alleviate the negative effects of AFB1 on the above parameters. The gut microbiota analysis indicated that AFB1 could significantly increase the abundances of Staphylococcus-xylosu, Esherichia-coli-g-Escherichia-Shigella, and decrease Lactobacillus-aviarius abundance (P < 0.05), but which were adjusted to almost the same levels as the control group by CMD addition. The correlative analysis showed that Lactobacillus-aviarius abundance was positively correlated with ADG, SOD and BF (P < 0.05), whereas Staphylococcus-xylosus abundance was positively correlated with AFB1 residues in serum and liver (P < 0.05). In conclusion, CMD could keep gut microbiota stable, alleviate histological lesions, increase growth performance, and reduce mycotoxin toxicity. The optimal CMD addition should be 1 g/kg in AFB1-contaminated broilers diet.http://www.sciencedirect.com/science/article/pii/S0032579122007283detoxifieraflatoxin B1broilerorgan damagegut microbiota |
| spellingShingle | Hongwei Guo Ping Wang Chaoqi Liu Juan Chang Qingqiang Yin Lijun Wang Sanjun Jin Qun Zhu Fushan Lu Compound mycotoxin detoxifier alleviating aflatoxin B1 toxic effects on broiler growth performance, organ damage and gut microbiota Poultry Science detoxifier aflatoxin B1 broiler organ damage gut microbiota |
| title | Compound mycotoxin detoxifier alleviating aflatoxin B1 toxic effects on broiler growth performance, organ damage and gut microbiota |
| title_full | Compound mycotoxin detoxifier alleviating aflatoxin B1 toxic effects on broiler growth performance, organ damage and gut microbiota |
| title_fullStr | Compound mycotoxin detoxifier alleviating aflatoxin B1 toxic effects on broiler growth performance, organ damage and gut microbiota |
| title_full_unstemmed | Compound mycotoxin detoxifier alleviating aflatoxin B1 toxic effects on broiler growth performance, organ damage and gut microbiota |
| title_short | Compound mycotoxin detoxifier alleviating aflatoxin B1 toxic effects on broiler growth performance, organ damage and gut microbiota |
| title_sort | compound mycotoxin detoxifier alleviating aflatoxin b1 toxic effects on broiler growth performance organ damage and gut microbiota |
| topic | detoxifier aflatoxin B1 broiler organ damage gut microbiota |
| url | http://www.sciencedirect.com/science/article/pii/S0032579122007283 |
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