Title: The L-type Ca2+ channel blocker nifedipine inhibits mycelial growth, sporulation, and virulence of Phytophthora capsici
The oomycete vegetable pathogen Phytophthora capsici causes significant losses of important vegetable crops worldwide. Calcium and other plant nutrients have been used in disease management of oomycete pathogens. Calcium homeostasis and signalling is essential for numerous biological processes, and...
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| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2016-08-01
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| Series: | Frontiers in Microbiology |
| Subjects: | |
| Online Access: | http://journal.frontiersin.org/Journal/10.3389/fmicb.2016.01236/full |
| _version_ | 1819153719476879360 |
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| author | Peiqing Liu jie gong xueling ding Yue Jiang guoliang chen benjin li qiyong weng Qinghe Chen |
| author_facet | Peiqing Liu jie gong xueling ding Yue Jiang guoliang chen benjin li qiyong weng Qinghe Chen |
| author_sort | Peiqing Liu |
| collection | DOAJ |
| description | The oomycete vegetable pathogen Phytophthora capsici causes significant losses of important vegetable crops worldwide. Calcium and other plant nutrients have been used in disease management of oomycete pathogens. Calcium homeostasis and signalling is essential for numerous biological processes, and Ca2+ channel blockers prevent excessive Ca2+ influx into the fungal cell. However, it is not known whether voltage-gated Ca2+ channel blockers improve control over oomycete pathogens. In the present study, we compared the inhibitory effects of CaCl2 and the extracellular Ca2+ chelator EDTA on mycelial growth and found that calcium assimilation plays a key role in P. capsici mycelial growth. Next, we involved the voltage-gated Ca2+ channel blockers verapamil (VP) and nifedipine (NFD) to analyse the effect of Ca2+ channel blockers on mycelial growth and sporulation; the results suggested that NFD, but not VP, caused significant inhibition. Ion rescue in an NFD-induced inhibition assay suggested that NFD-induced inhibition is calcium-dependent. In addition, NFD increased P. capsici sensitivity to H2O2 in a calcium-dependent manner, and extracellular calcium rescued it. Furthermore, NFD inhibited the virulence and gene expression related to its pathogenicity. These results suggest that NFD inhibits mycelial growth, sporulation, and virulence of P. capsici. |
| first_indexed | 2024-12-22T15:09:39Z |
| format | Article |
| id | doaj.art-b1648344edc9486b87ade36e9757c58a |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-22T15:09:39Z |
| publishDate | 2016-08-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-b1648344edc9486b87ade36e9757c58a2022-12-21T18:21:55ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2016-08-01710.3389/fmicb.2016.01236210241Title: The L-type Ca2+ channel blocker nifedipine inhibits mycelial growth, sporulation, and virulence of Phytophthora capsiciPeiqing Liu0jie gong1xueling ding2Yue Jiang3guoliang chen4benjin li5qiyong weng6Qinghe Chen7Fujian Academy of Agricultural SciencesFujian Academy of Agricultural SciencesFujian Academy of Agricultural SciencesFujian Academy of Agricultural SciencesFujian Academy of Agricultural SciencesFujian Academy of Agricultural SciencesFujian Academy of Agricultural SciencesFujian Academy of Agricultural SciencesThe oomycete vegetable pathogen Phytophthora capsici causes significant losses of important vegetable crops worldwide. Calcium and other plant nutrients have been used in disease management of oomycete pathogens. Calcium homeostasis and signalling is essential for numerous biological processes, and Ca2+ channel blockers prevent excessive Ca2+ influx into the fungal cell. However, it is not known whether voltage-gated Ca2+ channel blockers improve control over oomycete pathogens. In the present study, we compared the inhibitory effects of CaCl2 and the extracellular Ca2+ chelator EDTA on mycelial growth and found that calcium assimilation plays a key role in P. capsici mycelial growth. Next, we involved the voltage-gated Ca2+ channel blockers verapamil (VP) and nifedipine (NFD) to analyse the effect of Ca2+ channel blockers on mycelial growth and sporulation; the results suggested that NFD, but not VP, caused significant inhibition. Ion rescue in an NFD-induced inhibition assay suggested that NFD-induced inhibition is calcium-dependent. In addition, NFD increased P. capsici sensitivity to H2O2 in a calcium-dependent manner, and extracellular calcium rescued it. Furthermore, NFD inhibited the virulence and gene expression related to its pathogenicity. These results suggest that NFD inhibits mycelial growth, sporulation, and virulence of P. capsici.http://journal.frontiersin.org/Journal/10.3389/fmicb.2016.01236/fullNifedipineVirulenceH2O2Phytophthora capsicicalcium rescue |
| spellingShingle | Peiqing Liu jie gong xueling ding Yue Jiang guoliang chen benjin li qiyong weng Qinghe Chen Title: The L-type Ca2+ channel blocker nifedipine inhibits mycelial growth, sporulation, and virulence of Phytophthora capsici Frontiers in Microbiology Nifedipine Virulence H2O2 Phytophthora capsici calcium rescue |
| title | Title: The L-type Ca2+ channel blocker nifedipine inhibits mycelial growth, sporulation, and virulence of Phytophthora capsici |
| title_full | Title: The L-type Ca2+ channel blocker nifedipine inhibits mycelial growth, sporulation, and virulence of Phytophthora capsici |
| title_fullStr | Title: The L-type Ca2+ channel blocker nifedipine inhibits mycelial growth, sporulation, and virulence of Phytophthora capsici |
| title_full_unstemmed | Title: The L-type Ca2+ channel blocker nifedipine inhibits mycelial growth, sporulation, and virulence of Phytophthora capsici |
| title_short | Title: The L-type Ca2+ channel blocker nifedipine inhibits mycelial growth, sporulation, and virulence of Phytophthora capsici |
| title_sort | title the l type ca2 channel blocker nifedipine inhibits mycelial growth sporulation and virulence of phytophthora capsici |
| topic | Nifedipine Virulence H2O2 Phytophthora capsici calcium rescue |
| url | http://journal.frontiersin.org/Journal/10.3389/fmicb.2016.01236/full |
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