In Silico Study of Haematococcus pluvialis Biomarker Compound as Supplement to Fish Bone Remodelling
This study aims to determine the docking predictions for RAR, RXR, and ROR in the bone remodeling pathway using Haematococcus, which is the highest carotenoid-producing microalgae. Furthermore, it determines the projection of using this carotenoid-producing alga in bone development. Carotenoids inc...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Bogor Agricultural University
2022-03-01
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| Series: | Hayati Journal of Biosciences |
| Online Access: | https://journal.ipb.ac.id/index.php/hayati/article/view/39000 |
| _version_ | 1818308202324819968 |
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| author | Daniar Kusumawati Sri Widyarti Maftuch Maftuch Sri Rahayu |
| author_facet | Daniar Kusumawati Sri Widyarti Maftuch Maftuch Sri Rahayu |
| author_sort | Daniar Kusumawati |
| collection | DOAJ |
| description |
This study aims to determine the docking predictions for RAR, RXR, and ROR in the bone remodeling pathway using Haematococcus, which is the highest carotenoid-producing microalgae. Furthermore, it determines the projection of using this carotenoid-producing alga in bone development. Carotenoids include provitamin A and non-provitamin A, which are predicted to replace vitamin A in bone control. It is also required in silico proof of carotenoids' function of bone remodeling control. Furthermore, molecular and visualization docking validation was conducted using PyRx and Discovery Studio Visualizer software respectively. According to binding affinity and RMSD value, each biomarker compound had particular binding sites on RARα, RARβ, RORβ, and RORγ. Astaxanthin was the only compound with binding sites on all four receptors. Through enzymatic action, provitamin A carotenoids can serve as a precursor to retinol, allowing them to act as a native RXR ligand. Therefore, the biomarker compound used in Haematococcus pluvialis can replace the role of vitamin A in the regulation of fish bones. The prediction of bone regulation in biomarker compounds through the RAR-RXR pathways inhibited osteoblast and osteclast. Otherwise, VDR-RXR pathways regulated osteoclast maturation and osteoblast mineralization.
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| first_indexed | 2024-12-13T07:10:31Z |
| format | Article |
| id | doaj.art-b164f2fbf2f249a88014035e551a7d27 |
| institution | Directory Open Access Journal |
| issn | 1978-3019 2086-4094 |
| language | English |
| last_indexed | 2024-12-13T07:10:31Z |
| publishDate | 2022-03-01 |
| publisher | Bogor Agricultural University |
| record_format | Article |
| series | Hayati Journal of Biosciences |
| spelling | doaj.art-b164f2fbf2f249a88014035e551a7d272022-12-21T23:55:41ZengBogor Agricultural UniversityHayati Journal of Biosciences1978-30192086-40942022-03-0129310.4308/hjb.29.3.330-342In Silico Study of Haematococcus pluvialis Biomarker Compound as Supplement to Fish Bone RemodellingDaniar Kusumawati0Sri Widyarti1Maftuch Maftuch2Sri Rahayu3Department of Biology, Faculty of Mathematics and Natural Sciences, Brawijaya University, Malang, IndonesiaDepartment of Biology, Faculty of Mathematics and Natural Sciences, Brawijaya University, Malang, IndonesiaFaculty of Fisheries and Marine Science, Brawijaya University, Malang, IndonesiaDepartment of Biology, Faculty of Mathematics and Natural Sciences, Brawijaya University, Malang, Indonesia This study aims to determine the docking predictions for RAR, RXR, and ROR in the bone remodeling pathway using Haematococcus, which is the highest carotenoid-producing microalgae. Furthermore, it determines the projection of using this carotenoid-producing alga in bone development. Carotenoids include provitamin A and non-provitamin A, which are predicted to replace vitamin A in bone control. It is also required in silico proof of carotenoids' function of bone remodeling control. Furthermore, molecular and visualization docking validation was conducted using PyRx and Discovery Studio Visualizer software respectively. According to binding affinity and RMSD value, each biomarker compound had particular binding sites on RARα, RARβ, RORβ, and RORγ. Astaxanthin was the only compound with binding sites on all four receptors. Through enzymatic action, provitamin A carotenoids can serve as a precursor to retinol, allowing them to act as a native RXR ligand. Therefore, the biomarker compound used in Haematococcus pluvialis can replace the role of vitamin A in the regulation of fish bones. The prediction of bone regulation in biomarker compounds through the RAR-RXR pathways inhibited osteoblast and osteclast. Otherwise, VDR-RXR pathways regulated osteoclast maturation and osteoblast mineralization. https://journal.ipb.ac.id/index.php/hayati/article/view/39000 |
| spellingShingle | Daniar Kusumawati Sri Widyarti Maftuch Maftuch Sri Rahayu In Silico Study of Haematococcus pluvialis Biomarker Compound as Supplement to Fish Bone Remodelling Hayati Journal of Biosciences |
| title | In Silico Study of Haematococcus pluvialis Biomarker Compound as Supplement to Fish Bone Remodelling |
| title_full | In Silico Study of Haematococcus pluvialis Biomarker Compound as Supplement to Fish Bone Remodelling |
| title_fullStr | In Silico Study of Haematococcus pluvialis Biomarker Compound as Supplement to Fish Bone Remodelling |
| title_full_unstemmed | In Silico Study of Haematococcus pluvialis Biomarker Compound as Supplement to Fish Bone Remodelling |
| title_short | In Silico Study of Haematococcus pluvialis Biomarker Compound as Supplement to Fish Bone Remodelling |
| title_sort | in silico study of haematococcus pluvialis biomarker compound as supplement to fish bone remodelling |
| url | https://journal.ipb.ac.id/index.php/hayati/article/view/39000 |
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