Authentication of Bulbus Fritillariae Cirrhosae by RAPD-Derived DNA Markers
Bulbus Fritillariae is the most commonly used antitussive herb in China. Eleven species of Fritillaria are recorded as Bulbus Fritillariae in the Chinese Pharmacopoeia. Bulbus Fritillariae Cirrhosae is a group of six Fritillaria species with higher efficiency and lower toxicity derived mainly from w...
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| Format: | Article |
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MDPI AG
2014-03-01
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| Series: | Molecules |
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| Online Access: | http://www.mdpi.com/1420-3049/19/3/3450 |
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| author | Gui-Zhong Xin Yin-Ching Lam Maitinuer Maiwulanjiang Gallant K. L. Chan Kevin Yue Zhu Wai-Lun Tang Tina Ting-Xia Dong Zi-Qi Shi Ping Li Karl W. K. Tsim |
| author_facet | Gui-Zhong Xin Yin-Ching Lam Maitinuer Maiwulanjiang Gallant K. L. Chan Kevin Yue Zhu Wai-Lun Tang Tina Ting-Xia Dong Zi-Qi Shi Ping Li Karl W. K. Tsim |
| author_sort | Gui-Zhong Xin |
| collection | DOAJ |
| description | Bulbus Fritillariae is the most commonly used antitussive herb in China. Eleven species of Fritillaria are recorded as Bulbus Fritillariae in the Chinese Pharmacopoeia. Bulbus Fritillariae Cirrhosae is a group of six Fritillaria species with higher efficiency and lower toxicity derived mainly from wild sources. Because of their higher market price, five other Fritillaria species are often sold deceptively as Bulbus Fritillariae Cirrhosae in the herbal market. To ensure the efficacy and safety of medicinal herbs, the authentication of botanical resources is the first step in quality control. Here, a DNA based identification method was developed to authenticate the commercial sources of Bulbus Fritillariae Cirrhosae. A putative DNA marker (0.65 kb) specific for Bulbus Fritillariae Cirrhosae was identified using the Random Amplified Polymorphic DNA (RAPD) technique. A DNA marker representing a Sequence Characterized Amplified Region (SCAR) was developed from a RAPD amplicon. The SCAR marker was successfully applied to differentiate Bulbus Fritillariae Cirrhosae from different species of Fritillaria. Additionally, the SCAR marker was also useful in identifying the commercial samples of Bulbus Fritillariae Cirrhosae. Our results indicated that the RAPD-SCAR method was rapid, accurate and applicable in identifying Bulbus Fritillariae Cirrhosae at the DNA level. |
| first_indexed | 2024-12-23T20:36:21Z |
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| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-12-23T20:36:21Z |
| publishDate | 2014-03-01 |
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| record_format | Article |
| series | Molecules |
| spelling | doaj.art-b166bef46e4f4c959acdcf632c92c31d2022-12-21T17:32:05ZengMDPI AGMolecules1420-30492014-03-011933450345910.3390/molecules19033450molecules19033450Authentication of Bulbus Fritillariae Cirrhosae by RAPD-Derived DNA MarkersGui-Zhong Xin0Yin-Ching Lam1Maitinuer Maiwulanjiang2Gallant K. L. Chan3Kevin Yue Zhu4Wai-Lun Tang5Tina Ting-Xia Dong6Zi-Qi Shi7Ping Li8Karl W. K. Tsim9Division of Life Science and Center for Chinese Medicine, The Hong Kong University of Science and Technology, Clear Water Bay Road, Hong Kong, ChinaDivision of Life Science and Center for Chinese Medicine, The Hong Kong University of Science and Technology, Clear Water Bay Road, Hong Kong, ChinaDivision of Life Science and Center for Chinese Medicine, The Hong Kong University of Science and Technology, Clear Water Bay Road, Hong Kong, ChinaDivision of Life Science and Center for Chinese Medicine, The Hong Kong University of Science and Technology, Clear Water Bay Road, Hong Kong, ChinaDivision of Life Science and Center for Chinese Medicine, The Hong Kong University of Science and Technology, Clear Water Bay Road, Hong Kong, ChinaDivision of Life Science and Center for Chinese Medicine, The Hong Kong University of Science and Technology, Clear Water Bay Road, Hong Kong, ChinaDivision of Life Science and Center for Chinese Medicine, The Hong Kong University of Science and Technology, Clear Water Bay Road, Hong Kong, ChinaState Key Laboratory of Natural Medicines, China Pharmaceutical University, No. 24 Tongjia Lane, Nanjing 210009, ChinaState Key Laboratory of Natural Medicines, China Pharmaceutical University, No. 24 Tongjia Lane, Nanjing 210009, ChinaDivision of Life Science and Center for Chinese Medicine, The Hong Kong University of Science and Technology, Clear Water Bay Road, Hong Kong, ChinaBulbus Fritillariae is the most commonly used antitussive herb in China. Eleven species of Fritillaria are recorded as Bulbus Fritillariae in the Chinese Pharmacopoeia. Bulbus Fritillariae Cirrhosae is a group of six Fritillaria species with higher efficiency and lower toxicity derived mainly from wild sources. Because of their higher market price, five other Fritillaria species are often sold deceptively as Bulbus Fritillariae Cirrhosae in the herbal market. To ensure the efficacy and safety of medicinal herbs, the authentication of botanical resources is the first step in quality control. Here, a DNA based identification method was developed to authenticate the commercial sources of Bulbus Fritillariae Cirrhosae. A putative DNA marker (0.65 kb) specific for Bulbus Fritillariae Cirrhosae was identified using the Random Amplified Polymorphic DNA (RAPD) technique. A DNA marker representing a Sequence Characterized Amplified Region (SCAR) was developed from a RAPD amplicon. The SCAR marker was successfully applied to differentiate Bulbus Fritillariae Cirrhosae from different species of Fritillaria. Additionally, the SCAR marker was also useful in identifying the commercial samples of Bulbus Fritillariae Cirrhosae. Our results indicated that the RAPD-SCAR method was rapid, accurate and applicable in identifying Bulbus Fritillariae Cirrhosae at the DNA level.http://www.mdpi.com/1420-3049/19/3/3450Bulbus Fritillariae CirrhosaeRAPDSCARherbal drug authentication |
| spellingShingle | Gui-Zhong Xin Yin-Ching Lam Maitinuer Maiwulanjiang Gallant K. L. Chan Kevin Yue Zhu Wai-Lun Tang Tina Ting-Xia Dong Zi-Qi Shi Ping Li Karl W. K. Tsim Authentication of Bulbus Fritillariae Cirrhosae by RAPD-Derived DNA Markers Molecules Bulbus Fritillariae Cirrhosae RAPD SCAR herbal drug authentication |
| title | Authentication of Bulbus Fritillariae Cirrhosae by RAPD-Derived DNA Markers |
| title_full | Authentication of Bulbus Fritillariae Cirrhosae by RAPD-Derived DNA Markers |
| title_fullStr | Authentication of Bulbus Fritillariae Cirrhosae by RAPD-Derived DNA Markers |
| title_full_unstemmed | Authentication of Bulbus Fritillariae Cirrhosae by RAPD-Derived DNA Markers |
| title_short | Authentication of Bulbus Fritillariae Cirrhosae by RAPD-Derived DNA Markers |
| title_sort | authentication of bulbus fritillariae cirrhosae by rapd derived dna markers |
| topic | Bulbus Fritillariae Cirrhosae RAPD SCAR herbal drug authentication |
| url | http://www.mdpi.com/1420-3049/19/3/3450 |
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