The effect of subinhibitory concentrations of Satureja spp. essential oils on the biofilm formation and urease activity of Klebsiella pneumoniae
Background: Klebsiella pneumoniae is one of the most important bacteria that cause nosocomial infections. This opportunistic pathogen has a high potential for antibiotic resistance and can generate a thick layer of biofilm. Nowadays, antibiotic resistant strains are emerging and widely spreading wor...
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| Format: | Article |
| Language: | English |
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Institue of Medicinal Plants, ACECR
2020-03-01
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| Series: | Journal of Medicinal Plants |
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| Online Access: | http://jmp.ir/article-1-2610-en.html |
| _version_ | 1819107395817701376 |
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| author | Fateme Yazdani Iraj Rasooli Fatemeh Sefidkon Navid Saidi Parviz Owlia |
| author_facet | Fateme Yazdani Iraj Rasooli Fatemeh Sefidkon Navid Saidi Parviz Owlia |
| author_sort | Fateme Yazdani |
| collection | DOAJ |
| description | Background: Klebsiella pneumoniae is one of the most important bacteria that cause nosocomial infections. This opportunistic pathogen has a high potential for antibiotic resistance and can generate a thick layer of biofilm. Nowadays, antibiotic resistant strains are emerging and widely spreading worldwide. Thus, it is necessary to combat drug-resistant strains through the use of novel drugs (such as medicinal plants). Objective: This study aimed to evaluate the in vitro antibacterial activity of Satureja rechingeri Jamzad, S. khuzestanica Jamzad, S. bachtiarica Bunge, and S. mutica Fisch. & C.A.Mey. essential oils against K. pneumonia ATCC 700603. Methods: For evaluation of the minimal inhibitory concentration (MIC) of essential oils, broth microdilution method was used. The microtiter plate assay method was used for the assessment of anti-biofilm activities of sub-MIC value of essential oils. Colorimetric and Iodometric assays were used for determination of urease and beta-lactamase activity. Results: According to data collected, the MIC value of essential oils was 4096 µg/mL. sub-MIC value of essential oils inhibited biofilm formation and urease activity of K. pneumoniae. However, S. khuzestanica had more activity. None of the essential oils caused a significant decrease in beta-lactamase activity of K. pneumoniae. Conclusion: Based on our analysis S. khuzestanica had a good antibacterial, anti-biofilm activities and urease inhibitory effects against K. pneumoniae, but additional studies are required to investigate the exact mechanisms of the antibacterial action and functions of this phytocompound. |
| first_indexed | 2024-12-22T02:53:22Z |
| format | Article |
| id | doaj.art-b167553edf5c438ebd0623ed565b6c5d |
| institution | Directory Open Access Journal |
| issn | 2717-204X 2717-2058 |
| language | English |
| last_indexed | 2024-12-22T02:53:22Z |
| publishDate | 2020-03-01 |
| publisher | Institue of Medicinal Plants, ACECR |
| record_format | Article |
| series | Journal of Medicinal Plants |
| spelling | doaj.art-b167553edf5c438ebd0623ed565b6c5d2022-12-21T18:41:20ZengInstitue of Medicinal Plants, ACECRJournal of Medicinal Plants2717-204X2717-20582020-03-0119736370The effect of subinhibitory concentrations of Satureja spp. essential oils on the biofilm formation and urease activity of Klebsiella pneumoniaeFateme Yazdani0Iraj Rasooli1Fatemeh Sefidkon2Navid Saidi3Parviz Owlia4 Molecular Microbiology Research Center (MMRC), Faculty of Medicine, Shahed University, Tehran, Iran Molecular Microbiology Research Center, Faculty of Sciences, Shahed University, Tehran, Iran Research Institute of Forests and Rangelands, Agricultural Research Education and Extension Organization (AREEO), Tehran, Iran Molecular Microbiology Research Center (MMRC), Faculty of Medicine, Shahed University, Tehran, Iran Molecular Microbiology Research Center (MMRC), Faculty of Medicine, Shahed University, Tehran, Iran Background: Klebsiella pneumoniae is one of the most important bacteria that cause nosocomial infections. This opportunistic pathogen has a high potential for antibiotic resistance and can generate a thick layer of biofilm. Nowadays, antibiotic resistant strains are emerging and widely spreading worldwide. Thus, it is necessary to combat drug-resistant strains through the use of novel drugs (such as medicinal plants). Objective: This study aimed to evaluate the in vitro antibacterial activity of Satureja rechingeri Jamzad, S. khuzestanica Jamzad, S. bachtiarica Bunge, and S. mutica Fisch. & C.A.Mey. essential oils against K. pneumonia ATCC 700603. Methods: For evaluation of the minimal inhibitory concentration (MIC) of essential oils, broth microdilution method was used. The microtiter plate assay method was used for the assessment of anti-biofilm activities of sub-MIC value of essential oils. Colorimetric and Iodometric assays were used for determination of urease and beta-lactamase activity. Results: According to data collected, the MIC value of essential oils was 4096 µg/mL. sub-MIC value of essential oils inhibited biofilm formation and urease activity of K. pneumoniae. However, S. khuzestanica had more activity. None of the essential oils caused a significant decrease in beta-lactamase activity of K. pneumoniae. Conclusion: Based on our analysis S. khuzestanica had a good antibacterial, anti-biofilm activities and urease inhibitory effects against K. pneumoniae, but additional studies are required to investigate the exact mechanisms of the antibacterial action and functions of this phytocompound.http://jmp.ir/article-1-2610-en.html<i>k. pneumoniae</i><i>satureja</i>beta-lactamasebiofilmurease |
| spellingShingle | Fateme Yazdani Iraj Rasooli Fatemeh Sefidkon Navid Saidi Parviz Owlia The effect of subinhibitory concentrations of Satureja spp. essential oils on the biofilm formation and urease activity of Klebsiella pneumoniae Journal of Medicinal Plants <i>k. pneumoniae</i> <i>satureja</i> beta-lactamase biofilm urease |
| title | The effect of subinhibitory concentrations of Satureja spp. essential oils on the biofilm formation and urease activity of Klebsiella pneumoniae |
| title_full | The effect of subinhibitory concentrations of Satureja spp. essential oils on the biofilm formation and urease activity of Klebsiella pneumoniae |
| title_fullStr | The effect of subinhibitory concentrations of Satureja spp. essential oils on the biofilm formation and urease activity of Klebsiella pneumoniae |
| title_full_unstemmed | The effect of subinhibitory concentrations of Satureja spp. essential oils on the biofilm formation and urease activity of Klebsiella pneumoniae |
| title_short | The effect of subinhibitory concentrations of Satureja spp. essential oils on the biofilm formation and urease activity of Klebsiella pneumoniae |
| title_sort | effect of subinhibitory concentrations of satureja spp essential oils on the biofilm formation and urease activity of klebsiella pneumoniae |
| topic | <i>k. pneumoniae</i> <i>satureja</i> beta-lactamase biofilm urease |
| url | http://jmp.ir/article-1-2610-en.html |
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