Predominance of Beijing genotype in extensively drug resistant Mycobacterium tuberculosis isolates from a tertiary care hospital in New Delhi, India
Out of a total of 311 Mycobacterium tuberculosis isolates from sputum specimens subjected to first- and second-line drug-susceptibility testing (DST) at a hospital serving as a referral center for chronic tuberculosis (TB) cases in New Delhi, 232/311 (74.6%) isolates were found to be resistant to is...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2013-01-01
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| Series: | International Journal of Mycobacteriology |
| Subjects: | |
| Online Access: | http://www.ijmyco.org/article.asp?issn=2212-5531;year=2013;volume=2;issue=2;spage=109;epage=113;aulast=Arora |
| _version_ | 1811216035369451520 |
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| author | Jyoti Arora Manpreet Bhalla Zeeshan Sidiq Prabha Lal Digamber Behera Nalin Rastogi Vithal Prasad Myneedu |
| author_facet | Jyoti Arora Manpreet Bhalla Zeeshan Sidiq Prabha Lal Digamber Behera Nalin Rastogi Vithal Prasad Myneedu |
| author_sort | Jyoti Arora |
| collection | DOAJ |
| description | Out of a total of 311 Mycobacterium tuberculosis isolates from sputum specimens subjected to first- and second-line drug-susceptibility testing (DST) at a hospital serving as a referral center for chronic tuberculosis (TB) cases in New Delhi, 232/311 (74.6%) isolates were found to be resistant to isoniazid and rifampicin. Among multidrug-resistant (MDR) isolates, 119/232 (51.3%) were resistant to four first-line drugs (streptomycin, isoniazid, rifampicin and ethambutol). Mono-resistance to isoniazid was observed in 18 (5.7%) isolates, while none of the isolates tested showed mono-resistance to rifampicin. 50/232 (21.5%) isolates met the definition of extensively drug resistant (XDR) TB, i.e., additional resistance to a fluoroquinolone and at least one of the three injectable second-line drugs: kanamycin, capreomycin, or amikacin. Spoligotyping of the XDR-TB isolates revealed 14 patterns; 39/50 (78%) isolates being grouped in three clusters vs. 11/50 (22%) isolates being unique. SIT1/Beijing represented the largest cluster (n = 21, 42%), followed by SIT26/CAS1-Delhi (n = 10, 20%) and SIT 53/T1 (n = 8 isolates; 16%). This study corroborates recent observations from North India suggesting that both Beijing and CAS1-Delhi lineages constitute the bulk of XDR-TB isolates that are disseminating rapidly across a large geographical region in and around the capital city of India. |
| first_indexed | 2024-04-12T06:32:53Z |
| format | Article |
| id | doaj.art-b16976005ea841c981dbf991dcd81c40 |
| institution | Directory Open Access Journal |
| issn | 2212-5531 2212-554X |
| language | English |
| last_indexed | 2024-04-12T06:32:53Z |
| publishDate | 2013-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | International Journal of Mycobacteriology |
| spelling | doaj.art-b16976005ea841c981dbf991dcd81c402022-12-22T03:43:59ZengWolters Kluwer Medknow PublicationsInternational Journal of Mycobacteriology2212-55312212-554X2013-01-012210911310.1016/j.ijmyco.2013.03.001Predominance of Beijing genotype in extensively drug resistant Mycobacterium tuberculosis isolates from a tertiary care hospital in New Delhi, IndiaJyoti AroraManpreet BhallaZeeshan SidiqPrabha LalDigamber BeheraNalin RastogiVithal Prasad MyneeduOut of a total of 311 Mycobacterium tuberculosis isolates from sputum specimens subjected to first- and second-line drug-susceptibility testing (DST) at a hospital serving as a referral center for chronic tuberculosis (TB) cases in New Delhi, 232/311 (74.6%) isolates were found to be resistant to isoniazid and rifampicin. Among multidrug-resistant (MDR) isolates, 119/232 (51.3%) were resistant to four first-line drugs (streptomycin, isoniazid, rifampicin and ethambutol). Mono-resistance to isoniazid was observed in 18 (5.7%) isolates, while none of the isolates tested showed mono-resistance to rifampicin. 50/232 (21.5%) isolates met the definition of extensively drug resistant (XDR) TB, i.e., additional resistance to a fluoroquinolone and at least one of the three injectable second-line drugs: kanamycin, capreomycin, or amikacin. Spoligotyping of the XDR-TB isolates revealed 14 patterns; 39/50 (78%) isolates being grouped in three clusters vs. 11/50 (22%) isolates being unique. SIT1/Beijing represented the largest cluster (n = 21, 42%), followed by SIT26/CAS1-Delhi (n = 10, 20%) and SIT 53/T1 (n = 8 isolates; 16%). This study corroborates recent observations from North India suggesting that both Beijing and CAS1-Delhi lineages constitute the bulk of XDR-TB isolates that are disseminating rapidly across a large geographical region in and around the capital city of India.http://www.ijmyco.org/article.asp?issn=2212-5531;year=2013;volume=2;issue=2;spage=109;epage=113;aulast=AroraMycobacterium tuberculosisSpoligotypingExtensively drug resistant TBMultidrug-resistant TB |
| spellingShingle | Jyoti Arora Manpreet Bhalla Zeeshan Sidiq Prabha Lal Digamber Behera Nalin Rastogi Vithal Prasad Myneedu Predominance of Beijing genotype in extensively drug resistant Mycobacterium tuberculosis isolates from a tertiary care hospital in New Delhi, India International Journal of Mycobacteriology Mycobacterium tuberculosis Spoligotyping Extensively drug resistant TB Multidrug-resistant TB |
| title | Predominance of Beijing genotype in extensively drug resistant Mycobacterium tuberculosis isolates from a tertiary care hospital in New Delhi, India |
| title_full | Predominance of Beijing genotype in extensively drug resistant Mycobacterium tuberculosis isolates from a tertiary care hospital in New Delhi, India |
| title_fullStr | Predominance of Beijing genotype in extensively drug resistant Mycobacterium tuberculosis isolates from a tertiary care hospital in New Delhi, India |
| title_full_unstemmed | Predominance of Beijing genotype in extensively drug resistant Mycobacterium tuberculosis isolates from a tertiary care hospital in New Delhi, India |
| title_short | Predominance of Beijing genotype in extensively drug resistant Mycobacterium tuberculosis isolates from a tertiary care hospital in New Delhi, India |
| title_sort | predominance of beijing genotype in extensively drug resistant mycobacterium tuberculosis isolates from a tertiary care hospital in new delhi india |
| topic | Mycobacterium tuberculosis Spoligotyping Extensively drug resistant TB Multidrug-resistant TB |
| url | http://www.ijmyco.org/article.asp?issn=2212-5531;year=2013;volume=2;issue=2;spage=109;epage=113;aulast=Arora |
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