Protocorm Like Bodies (PLBs) dendrobium orchid “Gatton Sunray”

The Dendrobium Orchid Gatton Sunray is a hybrid orchid of Dendrobium pulchellum × Dendrobium illustre. A study was aimed to find the best type of medium and type of banana for the multiplication of Protocorm-Like Bodies (PLBs) of this orchid. This research was conducted from February to September 20...

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Main Authors: Romeida A., Ganefianti D.W., Marlin M., Sudjatmiko S., Hairani P.M., Herawati R., Rustikawati R., Supanjani S.
Format: Article
Language:English
Published: EDP Sciences 2023-01-01
Series:E3S Web of Conferences
Online Access:https://www.e3s-conferences.org/articles/e3sconf/pdf/2023/10/e3sconf_iseprolocal2023_03002.pdf
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author Romeida A.
Ganefianti D.W.
Marlin M.
Sudjatmiko S.
Hairani P.M.
Herawati R.
Rustikawati R.
Supanjani S.
author_facet Romeida A.
Ganefianti D.W.
Marlin M.
Sudjatmiko S.
Hairani P.M.
Herawati R.
Rustikawati R.
Supanjani S.
author_sort Romeida A.
collection DOAJ
description The Dendrobium Orchid Gatton Sunray is a hybrid orchid of Dendrobium pulchellum × Dendrobium illustre. A study was aimed to find the best type of medium and type of banana for the multiplication of Protocorm-Like Bodies (PLBs) of this orchid. This research was conducted from February to September 2020 at the Plant Tissue Culture Laboratory, Faculty of Agriculture, The University of Bengkulu. Treatments included type of media and cultivar of ripe banana fruit, added as source of organic complex, consisted of 4 cultivars, namely Ambon Curup, Kepok, Tanduk and Mas. Ripe bananas were mashed and added to the in vitro medium as much as 50 g.L-1. Types of media consist of Murashige and Skoog (MS), ½ Doses of Murashige and Skoog (MS) media, and Complex of fertilizer media. The results showed that the ½ MS medium added with 50 g.L-1 cv. Kepok fruit produced the greatest multiplication (11.8 PLBs per jar), the best growth of PLBs, including the greatest PLBs number (28.5 PLBs per jar), the heaviest fresh weight (1.30 g per jar) and the longest PLBs (2.56 cm).
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spelling doaj.art-b1697d1f25b84aea9b04e2fc26e53cf52023-03-21T10:31:46ZengEDP SciencesE3S Web of Conferences2267-12422023-01-013730300210.1051/e3sconf/202337303002e3sconf_iseprolocal2023_03002Protocorm Like Bodies (PLBs) dendrobium orchid “Gatton Sunray”Romeida A.0Ganefianti D.W.1Marlin M.2Sudjatmiko S.3Hairani P.M.4Herawati R.5Rustikawati R.6Supanjani S.7University of Bengkulu, Department of Plant Production, Faculty of AgricultureUniversity of Bengkulu, Department of Plant Production, Faculty of AgricultureUniversity of Bengkulu, Department of Plant Production, Faculty of AgricultureUniversity of Bengkulu, Department of Plant Production, Faculty of AgricultureUniversity of Bengkulu, Department of Plant Production, Faculty of AgricultureUniversity of Bengkulu, Department of Plant Production, Faculty of AgricultureUniversity of Bengkulu, Department of Plant Production, Faculty of AgricultureUniversity of Bengkulu, Department of Plant Production, Faculty of AgricultureThe Dendrobium Orchid Gatton Sunray is a hybrid orchid of Dendrobium pulchellum × Dendrobium illustre. A study was aimed to find the best type of medium and type of banana for the multiplication of Protocorm-Like Bodies (PLBs) of this orchid. This research was conducted from February to September 2020 at the Plant Tissue Culture Laboratory, Faculty of Agriculture, The University of Bengkulu. Treatments included type of media and cultivar of ripe banana fruit, added as source of organic complex, consisted of 4 cultivars, namely Ambon Curup, Kepok, Tanduk and Mas. Ripe bananas were mashed and added to the in vitro medium as much as 50 g.L-1. Types of media consist of Murashige and Skoog (MS), ½ Doses of Murashige and Skoog (MS) media, and Complex of fertilizer media. The results showed that the ½ MS medium added with 50 g.L-1 cv. Kepok fruit produced the greatest multiplication (11.8 PLBs per jar), the best growth of PLBs, including the greatest PLBs number (28.5 PLBs per jar), the heaviest fresh weight (1.30 g per jar) and the longest PLBs (2.56 cm).https://www.e3s-conferences.org/articles/e3sconf/pdf/2023/10/e3sconf_iseprolocal2023_03002.pdf
spellingShingle Romeida A.
Ganefianti D.W.
Marlin M.
Sudjatmiko S.
Hairani P.M.
Herawati R.
Rustikawati R.
Supanjani S.
Protocorm Like Bodies (PLBs) dendrobium orchid “Gatton Sunray”
E3S Web of Conferences
title Protocorm Like Bodies (PLBs) dendrobium orchid “Gatton Sunray”
title_full Protocorm Like Bodies (PLBs) dendrobium orchid “Gatton Sunray”
title_fullStr Protocorm Like Bodies (PLBs) dendrobium orchid “Gatton Sunray”
title_full_unstemmed Protocorm Like Bodies (PLBs) dendrobium orchid “Gatton Sunray”
title_short Protocorm Like Bodies (PLBs) dendrobium orchid “Gatton Sunray”
title_sort protocorm like bodies plbs dendrobium orchid gatton sunray
url https://www.e3s-conferences.org/articles/e3sconf/pdf/2023/10/e3sconf_iseprolocal2023_03002.pdf
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